Structure, Composition, & Histology Core - Core B

结构、组成、

• 批准号: 10676783
• 负责人: DAVID H. KOHN
• 金额: $ 25.22万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10676783
• 关键词: 3-Dimensional; Acceleration; Affect; Biological; Biological Assay; Blood Vessels; Cartilage; Cells; Cellular biology; Center Core Grants; Chemistry; Clinic; Collaborations; Communication; Communities; Data; Data Analyses; Development; Disease; Education; Education and Outreach; Engineering; Ensure; Experimental Designs; Fee-for-Service Plans; Future; Gene Expression; Genetic; Goals; Health; High Resolution Computed Tomography; Histologic; Histology; Image; Image Analysis; Interdisciplinary Study; Knowledge; Laboratories; Leadership; Length; Link; Measures; Mechanics; Michigan; Molecular; Morphology; Musculoskeletal; Needs Assessment; Organ; Outcome; Perfusion; Physiological; Process; Protocols documentation; Raman Spectrum Analysis; Recommendation; Recording of previous events; Reproducibility; Research; Research Personnel; Research Support; Resources; Science; Services; Standardization; Structure; Surveys; Technology; Testing; Tissues; Training; Translating; Universities; X-Ray Computed Tomography; biomedical imaging; bone; cost; functional outcomes; improved; innovation; instrument; microCT; nano; nanoscale; neural network; physical science; programs; repository; response; tool; trait; treatment strategy; virtual

Michigan Integrative Musculoskeletal Health Core Center – Core B Project Summary Structure and composition across physiological length-scales are key intermediate traits that link gene expression with functional outcomes. Genetic and/or environmental perturbations affecting gene expression often lead to measureable changes in these intermediate traits that have mechanical and other functional consequences. Accurately quantifying structure and composition at different length scales is therefore essential to advancing our understanding of the molecular and mechanical mechanisms underlying musculoskeletal health and disease; to develop and evaluate treatment strategies; and to ultimately translate these strategies to the clinic. However, this research requires use of advanced and costly instruments and supporting expertise in cell biology, chemistry, engineering, and biomedical imaging. The Structure, Composition and Histology Core (Core B) will provide access to the instruments and expertise that will enable Center investigators to quantify these intermediate traits and to accelerate their research programs. Core B will include ex-vivo micro-Computed Tomography (µCT) and nano-Computed Tomography (nano-CT), Raman spectroscopy, and histology as its major components. These technologies were chosen for their applicability to all musculoskeletal tissues and based on usage data over the previous 4 years and a needs-assessment survey of core users. Investigators at the University of Michigan pioneered the use of µCT and Raman spectroscopy to investigate musculoskeletal tissues and are leaders in applying these technologies to solve musculoskeletal problems. Histology continues to be the gateway approach to understanding changes in tissue morphology and, when combined with CT and Raman spectroscopy, histology can link tissue morphology to composition and functional outcomes. The goals of this Core are to leverage the expertise of the core leadership to support the research programs of Center investigators in performing structural, compositional and histological analyses of all musculoskeletal tissues, both intellectually and financially; to expand opportunities for our research community; and to bring new investigators into the musculoskeletal community. To accomplish our goals and to continue creating a robust and sustainable impact to the musculoskeletal community in the P30 renewal application, we propose the following Specific Aims: Aim 1: To provide expertise and guidance on the best use of technologies and experimental design to allow investigators to conduct specialized assays for quantifying structure and composition across length scales. Aim 2: To provide expert use, standardization, and training in state-of-the-art technologies to quantify structure and composition across physiological length scales. Aim 3: To provide expertise and scientific guidance on data analysis, interpretation of results and recommendations for future studies. Aim 4: To expand opportunities for our research community through innovation and development of enabling technologies, education, training, and enrichment.

密歇根综合肌肉骨骼健康核心中心-核心B 项目摘要 跨生理长度尺度的结构和组成是连接基因的关键中间性状 表达与功能结果。影响基因表达的遗传和/或环境扰动 通常会导致这些中间性状的可测量变化，这些中间性状具有机械和其他功能， 后果因此，在不同的长度尺度上准确地量化结构和组成是必不可少的 促进我们对肌肉骨骼健康的分子和机械机制的理解 和疾病;制定和评估治疗策略;并最终将这些策略转化为 诊所然而，这项研究需要使用先进和昂贵的仪器和支持细胞的专业知识， 生物学、化学、工程学和生物医学成像。结构、组成和组织学核心 (Core B）将提供工具和专业知识，使中心研究者能够量化 这些中间性状，并加快他们的研究计划。核心B将包括离体微计算 断层扫描（µCT）和纳米计算机断层扫描（nano-CT）、拉曼光谱和组织学作为其 主要组成部分。选择这些技术是因为它们适用于所有肌肉骨骼组织， 根据过去4年的使用数据和对核心用户的需求评估调查。的调查人员 密歇根大学率先使用微CT和拉曼光谱来研究肌肉骨骼 是应用这些技术解决肌肉骨骼问题的领导者。组织学继续 是了解组织形态变化的入门方法，当与CT结合时， 拉曼光谱、组织学可以将组织形态与组成和功能结果联系起来。的目标 该核心的主要任务是利用核心领导层的专业知识来支持中心的研究计划。 研究人员对所有肌肉骨骼组织进行结构、成分和组织学分析， 智力和财政;扩大我们的研究界的机会;并带来新的研究人员 进入肌肉骨骼系统。为了实现我们的目标，并继续创造一个强大的和可持续的 在P30续期申请中，我们提出了以下具体目标： 目标1：就技术和实验设计的最佳使用提供专门知识和指导， 研究人员进行专门的测定，以量化跨长度尺度的结构和组成。 目标2：提供最先进技术的专家使用、标准化和培训，以量化结构 和生理长度尺度上的组成。 目标3：就数据分析、结果解释和 对今后研究的建议。 目标4：通过创新和发展，为我们的研究界扩大机会， 技术、教育、培训和丰富。