Antibiotic Resistance Surveillance in Retail Food Specimens in the Southwestern United States: Texas, Oklahoma, and New Mexico

美国西南部零售食品样品的抗生素耐药性监测 州：德克萨斯州、俄克拉何马州和新墨西哥州 对不同抗菌剂的耐药性的流行率和多样性可能有很大差异。 在国家和地区之间，这可能是由于抗菌剂方面的实质性差异 使用习惯。因此，这项研究的目标是确定抗菌素的普及率 零售分离的沙门氏菌、弯曲杆菌、大肠埃希菌和肠球菌的耐药性 年在杂货店购买的鸡肉、火鸡末、牛肉末和猪排的样本 美国西南部，包括德克萨斯州、俄克拉何马州和新墨西哥州，使用标准化的 方法，以便可以与其他位置进行比较。实现这一目标将有助于 我们对零售肉类和食品中抗生素抗药性细菌的负担和数量的理解 禽类产品分布在美国西南部。具体地说， 这样做的结果是 该项目还将提供分离株，供国家监测计划进一步鉴定 作为区域和国家抗菌素耐药性(AMR)风险评估的新数据 ，并将探索AMR风险管理选项，重点放在教育和 在美国西南部接受培训。在合作协议的五年期间， 每月抽取不超过80个肉类及家禽样本如下：零售鸡只 (40)，火鸡末(20)，牛肉末(10)和猪排(10)。我们将收集样本 在德克萨斯州(阿马里洛，奥斯汀，科珀斯克里斯蒂，达拉斯，沃斯堡，埃尔帕索，休斯顿，卢伯克， 米德兰、敖德萨和圣安东尼奥)、俄克拉何马州(俄克拉何马城和塔尔萨)和新墨西哥州 (阿尔伯克基、拉斯克鲁塞斯、里奥兰乔和圣达菲)。选择主要城市是因为 对这些地区人口的公共卫生影响，因此样本将是 代表更多的个体。样本将由ICFIE人员采集 并在24小时内被送回德克萨斯理工大学ICFIE实验室 立即使用FDA-NARMS方案进行细菌检测和分离。在…的末尾 本合作协议的五年，进一步AMR的细菌学数据库 FDA和TTU的特性都将得到极大的改进和扩展。此外， 数据趋势分析将使当地和联邦研究人员能够确定耐药细菌是否 聚集在该国的某些城市或地区。最后，这个项目的结果将会有所改善 当地生产商和零售商关于抗生素耐药性的教育活动和知识。

Antibiotic Resistance Surveillance in Retail Food Specimens in the Southwestern United States: Texas, Oklahoma, and New Mexico The prevalence and diversity of resistance to different antimicrobials can vary greatly among countries and regions, and this might be due to substantive differences in antimicrobial usage practices. Therefore, the goal of this study is to determine the prevalence of antimicrobial resistance among Salmonella, Campylobacter, E. coli and Enterococci isolated from retail samples of chicken, ground turkey, ground beef and pork chops purchased from grocery stores in the Southwestern United States including Texas, Oklahoma, and New Mexico using standardized methods so comparisons can be made to other locations. Achieving this goal will contribute to our understanding of the burden and magnitude of antibiotic resistant bacteria, in retail meat and poultry products distributed in the Southwestern United States. Specifically, the results of this project will provide isolates for further characterization by national monitoring programs as well as novel data to inform regional and national risk assessments on antimicrobial resistance (AMR) in animal products, and will explore AMR risk management options focused on education and training in the Southwestern United States. During the five years of the Cooperative Agreement, no more than 80 meat and poultry samples will be collected per month as follows: retail chicken (40), ground turkey (20), ground beef 80/20 (10), and pork chops (10). Samples will be collected in Texas (Amarillo, Austin, Corpus Christi, Dallas, Fort Worth, El Paso, Houston, Lubbock, Midland, Odessa, and San Antonio), Oklahoma (Oklahoma City and Tulsa), and New Mexico (Albuquerque, Las Cruces, Rio Rancho, and Santa Fe). Major cities were chosen due to the public health impact on the population in those areas and hence the samples would be representative of a greater number of individuals. Samples will be collected by ICFIE personnel and transported back to the Texas Tech ICFIE laboratory within 24 hours and processed immediately using FDA-NARMS protocols for bacterial detection and isolation. At the end of the five years of this Cooperative Agreement, the bacteriological database for further AMR characterization, both at FDA and TTU, will be greatly improved and expanded. Furthermore, data trend analysis will allow local and federal researchers to determine if resistant bacteria are clustered in certain cities or areas of the country. Lastly, the results of this project will improve educational activities and knowledge of local producers and retailers on antibiotic resistance.