Antibiotic Resistance Surveillance in Retail Food Specimens in Texas and Oklahoma

德克萨斯州零售食品样本中的抗生素耐药性监测 俄克拉荷马州 项目摘要 国家抗生素耐药性监测系统（NARMS），一个监测 该系统建立于1996年，跟踪人类肠道细菌的抗生素耐药性， 零售肉类和食用动物，以及其对新兴细菌的不同计划， 抵抗力促进和保护公众健康。本研究的目的是确定 沙门氏菌、弯曲杆菌、大肠埃希菌的耐药性（AMR）流行情况; 从鸡只、火鸡、牛肉和猪肉的零售样本中分离出大肠杆菌和肠球菌， 从肠球菌、弧菌、气单胞菌和乳糖发酵菌中提取海产品 使用标准化方法从德克萨斯州和俄克拉荷马州的杂货店购买， 可以与参与NARMS食品的其他国家地点进行比较 监视程序实现这一目标将有助于我们了解 零售肉类、家禽和海鲜中耐抗生素细菌的负担和数量 在该地区分销和销售给消费者的产品约包括 占美国人口的10%。具体而言，该项目的成果将 向FDA-CVM提供分离株和测序数据（全基因组测序） 从重要的公共卫生肠道细菌，帮助进行知情的风险 动物产品的抗菌素耐药性（AMR）评估。在每年的 根据合作协议，德克萨斯理工大学食品微生物实验室将 获取食物样本如下：120只带骨带皮鸡肉，120只火鸡肉，120只 碎牛肉，120块碎猪肉或猪排，24只虾，36条鲑鱼和36条罗非鱼，所有 在传统、有机或自然生产系统下生产。样品 将每月在德克萨斯州拉伯克400英里（6小时车程）内收集，包括 小都市、城市、郊区和农村城镇以及大、中、 小杂货店，超市，和整个地区的独立杂货店， 将增加多样化取样区域的代表性。采集的样本将 使用经批准的FDA-NARMS方案进行细菌检测和分离 将每月运送到FDA-CVM进行AMR测试，而测序数据将 用于进一步分析和分子测定抗微生物剂耐药性。的 该项目的成果将改善和增加教育活动和知识， 抗生素耐药性以及提高实验室检测方法的能力 和政府实验室使用的协议。

Antibiotic Resistance Surveillance in Retail Food Specimens in Texas and Oklahoma Project Summary The National Antimicrobial Resistance Monitoring System (NARMS), a surveillance system established in 1996, tracks antibiotic resistance in enteric bacteria from humans, retail meats, and food animals, and its different programs on emerging bacterial resistance promote and protect public health. The goal of this study is to determine the prevalence of antimicrobial resistance (AMR) among Salmonella, Campylobacter, E. coli and Enterococcus isolated from retail samples of chicken, turkey, beef and pork and from Enterococcus, Vibrio, Aeromonas and lactose fermenting bacteria from seafood purchased from grocery stores in Texas and Oklahoma using standardized methods so comparisons can be made to other national locations participating in the NARMS food surveillance program. Achieving this goal will contribute to our understanding of the burden and magnitude of antibiotic resistant bacteria in retail meat, poultry and seafood products distributed and sold to consumers in this region that comprises approximately 10% of the population of the United States. Specifically, the results of this project will provide the FDA-CVM with isolates and sequencing data (whole genome sequencing) from important public health enteric bacteria, helping to conduct informed risk assessments on antimicrobial resistance (AMR) in animal products. During each year of the Cooperative Agreement, the Texas Tech University food microbiology laboratory will obtain food samples as follows: 120 bone-in, skin-on chicken, 120 ground turkey, 120 ground beef, 120 ground pork or pork chops, 24 shrimp, 36 salmon, and 36 tilapia, all produced under either conventional, organic or natural production systems. Samples will be collected monthly within 400 miles (6-hour drive) of Lubbock, Texas, including small metro, urban, suburban and rural cities and towns as well as large, medium and small grocery stores, supermarkets, and independent grocers throughout the region that will add representativeness to the diverse sampling region. Collected samples will be processed using approved FDA-NARMS protocols for bacterial detection, and isolates will be shipped monthly to FDA-CVM for AMR testing, while sequencing data will be used for further analysis and molecular determination of antimicrobial resistance. The results of this project will improve and increase educational activities and knowledge on antibiotic resistance as well as improve laboratory capacity on testing methodologies and protocols used by government laboratories.