Toolkit for Fast, Multipurpose and Inducible Bioorthogonal Chemistry
快速、多用途和诱导生物正交化学工具包
基本信息
- 批准号:9899272
- 负责人:
- 金额:$ 35.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2023-01-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAcuteAffinityBindingBiological Response Modifier TherapyBiologyBiotechnologyBlood CirculationCC49 antibodyCell surfaceCellsChelating AgentsChemicalsChemistryColon CarcinomaCouplingDevelopmentDiseaseDrug Delivery SystemsDrug KineticsEnvironmentEnzymesGenerationsGoalsHeatingIn SituLabelLigandsLigationLightLocalesMediatingMethodologyMethodsModelingN-terminalPermeabilityPharmaceutical PreparationsPlant ResinsProceduresProdrugsPropertyProtein EngineeringProteinsProteomicsRadiochemistryReactionReagentReporterRoleSafetySideSiteSolubilitySurfaceSystemTechniquesTechnologyTimeTissuesVisible RadiationWorkantibody conjugatebasecancer cellcatalystcytotoxicdesigndrug candidatefluorophorefunctional groupimprovedinterestmaterials sciencenew technologyprotein purificationreceptorsmall moleculetissue culturetooltrafficking
项目摘要
Project Summary
The goal of this proposal will be to develop a diverse set of new chemical tools centered
on tetrazine ligation– the fastest known bioorthogonal reaction. This proposal describes
new tools for the safe synthesis and direct coupling of `minimalist' tetrazines to any
molecule of interest, including API's and fluorescent reporters. We also propose to
develop specialized tetrazines that can serve a dual role in enabling protein purification
followed by subsequent site-selective bioorthogonal chemistry. Finally, we propose to
develop efficient catalytic methods for `turning on' rapid bioorthogonal chemistry in
cellular context. We will develop tool molecules with high stability in the cellular
environment in their `off' state, and the fastest bioorthogonal reactions to date in their `on'
state. An application of the catalytically inducible tetrazine ligation is new method of
drug release where a photocatalyst-antibody conjugate is pretargeted to a disease site,
and red light is used to drive the local release of a cytotoxic reagent from a photocaged
prodrug.
项目摘要
这项提议的目标将是开发一套多样化的新化学工具
关于四嗪连接--已知最快的生物正交反应。这份提案描述了
安全合成和直接偶联“极简”四嗪的新工具
感兴趣的分子,包括API和荧光记者。我们还建议
开发可以在蛋白质纯化方面起双重作用的专业四嗪类药物
然后是随后的位置选择性生物正交化学。最后,我们建议
开发快速生物正交化学的高效催化方法
蜂窝环境。我们将开发在细胞内具有高度稳定性的工具分子
处于关闭状态的环境,以及迄今为止处于打开状态的最快生物正交反应
州政府。催化诱导四嗪结扎法的一种应用
药物释放,其中光催化剂-抗体结合物被预先定位于疾病部位,
红光用于驱动细胞毒性试剂从光笼中的局部释放
前药。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JOSEPH M FOX其他文献
JOSEPH M FOX的其他文献
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{{ truncateString('JOSEPH M FOX', 18)}}的其他基金
Toolkit for Fast, Multipurpose and Inducible Bioorthogonal Chemistry
快速、多用途和诱导生物正交化学工具包
- 批准号:
10660115 - 财政年份:2019
- 资助金额:
$ 35.88万 - 项目类别:
NIH ADMINISTRATIVE SUPPLEMENT FPLC SYSTEM FOX
NIH 行政补充 FPLC 系统 FOX
- 批准号:
9925864 - 财政年份:2019
- 资助金额:
$ 35.88万 - 项目类别:
Supplement to Toolkit for Fast, Multipurpose and Inducible Bioorthogonal Chemistry
快速、多用途和诱导生物正交化学工具包的补充
- 批准号:
10046448 - 财政年份:2019
- 资助金额:
$ 35.88万 - 项目类别:
Toolkit for Fast, Multipurpose and Inducible Bioorthogonal Chemistry
快速、多用途和诱导生物正交化学工具包
- 批准号:
10343710 - 财政年份:2019
- 资助金额:
$ 35.88万 - 项目类别:
Discovery of Molecular Probes and Therapeutic Leads, Administrative Supplement for Equipment
分子探针和治疗先导物的发现,设备管理补充
- 批准号:
10400299 - 财政年份:2014
- 资助金额:
$ 35.88万 - 项目类别:
Discovery of Chemical Probes and Therapeutic Leads, Phase 2
化学探针和治疗先导化合物的发现,第二阶段
- 批准号:
10853967 - 财政年份:2014
- 资助金额:
$ 35.88万 - 项目类别:
Discovery of Chemical Probes and Therapeutic Leads, Phase II
化学探针和治疗先导化合物的发现,第二阶段
- 批准号:
10654734 - 财政年份:2014
- 资助金额:
$ 35.88万 - 项目类别:
Discovery of Chemical Probes and Therapeutic Leads: Cryoprobe Upgrade of a 400 MHz NMR
化学探针和治疗先导化合物的发现:400 MHz NMR 的冷冻探针升级
- 批准号:
10581950 - 财政年份:2014
- 资助金额:
$ 35.88万 - 项目类别:
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