Role of Retinoic Acid in the Regulation of the Blood-Retinal Barrier.

视黄酸在血视网膜屏障调节中的作用。

• 批准号: 9769753
• 负责人: BELA ANAND-APTE
• 金额: $ 41.3万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9769753
• 关键词: Address; Astrocytes; Biological Models; Blindness; Blood Tests; Blood Vessels; Blood-Retinal Barrier; Cells; Communication; Cues; Cultured Cells; Data; Development; Developmental Process; Disease; Endothelial Cells; Environment; Enzymes; Extravasation; Genes; Genetic; Goals; Hand; Health; Hemorrhage; Imagery; In Situ; Laboratories; Lasers; Lead; Maintenance; Mammals; Modeling; Molecular; Natural regeneration; Ophthalmoscopy; Pathway interactions; Pattern; Perfusion; Pericytes; Phase; Phenotype; Physiological; Physiological Processes; Plasma; Play; Process; Production; Property; Regulation; Reporting; Research; Retina; Retinal; Rodent; Role; Scanning; Signal Pathway; Signal Transduction; Testing; Tight Junctions; Time; Tissues; Transgenic Organisms; Tretinoin; Validation; Vegf Inhibitor; WNT Signaling Pathway; Zebrafish; base; course development; enhanced green fluorescent protein; in vitro Model; in vivo; in vivo Model; in vivo imaging; insight; neurovascular unit; novel; overexpression; retina blood vessel structure; retinal imaging; seal; tool

Project Summary An intact blood-retinal barrier (BRB) is critical for the maintenance of the health of the retina. The endothelial cells of the retinal blood vessels along with the surrounding pericytes and astrocytes form a neurovascular unit with strong barrier properties. Very little is known about the molecular and cellular mechanisms that regulate the development and maintenance of the BRB. We hypothesize that retinoic acid (RA) plays a critical role in the regulation of BRB formation as well as maintenance. We will test this hypothesis using a novel zebrafish model that we have developed, which allows the longitudinal in vivo visualization and testing of the BRB.

项目摘要 一个完整的血-视网膜屏障(BRB)对于维持视网膜的健康至关重要。内皮细胞 视网膜血管的细胞以及周围的周细胞和星形胶质细胞形成一个神经血管单位。 具有很强的阻隔性。人们对调控的分子和细胞机制知之甚少。 BRB的开发和维护。我们假设维甲酸(RA)在 BRB的形成和维护的调节。我们将使用一种新的斑马鱼来验证这一假设 我们开发的模型，它允许在体内纵向可视化和测试BRB。