Cell, Tissue and Animal Core

细胞-组织-动物芯 摘要 该核心的主要目标是为CHAIN提供原代细胞培养和动物模型 研究人员执行他们的研究项目，研究慢性艾滋病毒感染/衰老/ cART对 脑部疾病我们长期以来一直为中心提供来自啮齿动物和人类的优质原代细胞 调查员使用。我们新开发了人源化NSG小鼠模型， 其他啮齿类神经艾滋病模型。我们有多种技术和测定方法， 啮齿动物和非人类灵长类动物模型，是询问CHAIN和相关研究的宝贵工具 问题.这个核心提供和支持的细胞和模型在我们的许多领域都是必不可少的。 神经艾滋病的研究通过使用成像和组学核心，研究人员可以研究 疾病的进展和动物模型中神经病理学的分子决定因素。状态 还提供了分析的现有技术方法。这个核心的总体目标是：1）支持中心 研究人员通过提供来自动物的原代人类和啮齿动物血液和脑细胞和组织， 神经艾滋病模型; 2）与CHAIN研究人员合作研究慢性 艾滋病毒中枢神经系统感染，以及3）提供服务/培训原位脑组织分析，量化 SIV/HIV病毒RNA、DNA载量和许多其他免疫学和病毒学检测， 研究CNS疾病。

Cell-Tissue-Animal Core Abstract The primary objective of this core is providing primary cell culture and animal models to CHAIN investigators to execute their research projects on the effect of chronic HIV infection/ aging/ cART on brain disease. We have long provided quality primary cells from rodents and humans for Center investigator use. We have newly developed Humanized NSG mouse model and have experience in other rodent models of neuroAIDS. We have multiple techniques and assays enabling the study of both rodent and nonhuman primate models, valuable tools to ask CHAIN and related research questions. The cells and models provided and supported by this Core are essential in many our investigators’ neuroAIDS studies. By employing Imaging and Omics Cores, investigators can study progression of disease and molecular determinants of neuropathology in animal models. State of the art methods of analyses are also provided. The general goals of this core is 1) to support the center investigators by providing primary human and rodent blood and brain cells and tissues from animal models of neuroAIDS; 2) to work with CHAIN investigators in the study of animal models of chronic HIV CNS infection, and 3) to provide services/training in situ brain tissue analyses, quantification for SIV/HIV viral RNA, DNA loads, and many other immunological and virological assays required to study CNS disorders.