TRD #1: New approaches to specimen preparation

TRD

• 批准号: 9905529
• 负责人: CLINTON S POTTER
• 金额: $ 23.39万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9905529
• 关键词: Communities; Consumption; Copper; Coupled; Devices; Drops; Film; Gold; Length; Location; Methods; Microfluidics; Microscopy; Molecular; Negative Staining; Paper; Performance; Preparation; Reaction; Reproducibility; Research; Resources; Sampling; Specimen; Spottings; Stains; Standardization; System; Testing; Thinness; Time; Time Management; Variant; density; design; experimental study; improved; nanolitre; nanowire; novel; novel strategies; robotic device; screening; success

2. TRD#1: New Approaches to Specimen Preparation Abstract We are developing novel methods for specimen preparation that provide several advantages over traditional methods. These advantages include consumption of very small amounts of sample, and providing for more control over the quality of the prepared grids. The system uses a robotic device, called Spotiton, to dispense drops with volumes on the order of nanoliters to precise locations on an EM grid. The success of the device is coupled to a new “self-blotting” grid that we have developed to provide a method for spreading sample to a thin film without the use of externally applied filter paper. Over the next 5 years we will design, develop, test and validate a generalized Spotiton system capable of multiple tasks including making well controlled vitrified grids, managing time resolved and fast mixing experiments, and preparing ~100 independent negatively stained samples on a single grid. We anticipate that a version of this device will be manufactured by a commercial company so that it becomes generally available to the research community.

2. TRD#1：样品制备的新方法 抽象的 我们正在开发新的样本制备方法，与传统方法相比具有多种优势 方法。这些优点包括消耗非常少量的样品，并提供更多 控制准备好的网格的质量。该系统使用名为 Spotiton 的机器人设备来分配 以纳升量级的体积掉落到电磁网格上的精确位置。该装置的成功在于 与我们开发的新的“自印迹”网格相结合，以提供一种将样品分散到薄层的方法 不使用外部滤纸的薄膜。在接下来的5年里，我们将设计、开发、测试和 验证通用 Spotiton 系统能够执行多项任务，包括制作良好控制的玻璃化网格， 管理时间分辨和快速混合实验，并准备约 100 个独立的负染色 单个网格上的样本。我们预计该设备的一个版本将由商业公司制造 公司，以便研究界可以普遍使用它。