ShEEP request for High Performance Electrophysiological System for Recording and Closed-Loop Stimulation

在本次股票设备授予申请中，我们建议购买并验证高 自由行为小鼠表演多道电生理记录/刺激系统 以及执行行为任务的老鼠。这台设备的用处是扩大我们的电流 在进行行为测试时评估大脑活动的神经行为核心设施。作为一名 SFVAMC的一组调查人员对中风、创伤性脑损伤和 神经退行性变，我们正在寻找中枢神经系统功能的体内相关性。确定功能 脑损伤或变性后的预后和相应的治疗，我们已经建立了一个 神经行为核心设施，提供全面的运动和认知电池 功能测试。然而，这些测试仍然不够具体，无法破译确切的大脑回路。 与行为有关，它们也不够敏感，无法检测到 细胞分辨率。我们建议在行为自由的小鼠中加入大规模神经记录 以及执行行为任务的大鼠，因为大型合奏录音对功能 电路映射。建议的系统能够处理多达512个通道的数据，具有 配对通道主控台，用于自由活动的啮齿动物，能够记录多达512个通道。 凭借同时记录神经活动和感知行为的能力，该系统可以 还允许使用闭合环刺激和/或通过 光遗传学技术，梳理出与脑损伤有关的特定电路或振荡类型 或者成为治疗的靶子。这种尖端的多通道电生理记录和 刺激系统不仅将加深我们对脑损伤/变性影响的理解 神经回路，但也将扩大我们的研究计划，寻找治疗干预措施 这些疾病通常困扰着我们的退伍军人。

In this share equipment grant application, we propose to purchase and validate a high performance multichannel electrophysiology recording/stimulation system for free behaving mice and rats performing behavioral tasks. The utility of this equipment is to extend our current neurobehavioral core facility in assessing brain activity while performing behavioral tests. As a group of investigators at the SFVAMC interested in stroke, traumatic brain injury and neurodegeneration, we are seeking an in-vivo correlate of CNS function. To determine functional outcome after brain injury or degeneration and respective treatment, we have built a neurobehavioral core facility that provides a comprehensive battery of motor and cognitive function tests. However, these tests are still not specific enough to decipher the exact brain circuit involved in behavior, neither are they sensitive enough to detect neuronal impairment at the cellular resolution. We propose to incorporate large-scale neural recording in freely behaving mice and rats performing behavioral tasks because large ensemble recordings are critical for functional circuit mapping. The proposed system is capable of processing up to 512 channels of data, with matching-channel headstages for freely moving rodents capable of recording up to 512 channels. With the ability in simultaneously recording neural activity and sensing behavior, this system can also allow precise control of neural circuit of interest using closed-loop stimulation and/or with optogenetics techniques, to tease out specific circuitry or type of oscillation involved in brain injury or being targeted in therapy. This cutting-edge multichannel electrophysiology recording and stimulation system will not only better our understanding in how brain injury/degeneration affects neural circuits, but also will extend our research programs in finding therapeutic interventions for these disorders that commonly afflicting our veterans.