权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Spatial and single cell analysis of tumor microenvironment by seqFISH+

通过 seqFISH 对肿瘤微环境进行空间和单细胞分析

基本信息

批准号：
9909945
负责人：
Kirsten Frieda
金额：
$ 100万
依托单位：
SPATIAL GENOMICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-01 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9909945
关键词：
Atlases Bar Codes Biological Models CD28 gene Cancer Biology Cells Childhood Cities Collaborations Complex Computer software Data Disease Progression Disease Resistance Ecosystem Engineering Fluorescent in Situ Hybridization Fostering Gene Expression Profile Genes Genome Genomics Geography Heterogeneity Human Image Immune Immune system Immunologic Surveillance Immunooncology Immunotherapeutic agent Immunotherapy In Situ Individual Infiltration Knowledge Letters Malignant Neoplasms Maps Messenger RNA Methods Microscope Molecular Mus Nature Neoplasm Metastasis Neoplastic Cell Transformation Neuroblastoma Oncology Group Pathogenesis Pathology Pathway interactions Pattern Pediatric Hospitals Play Population RNA Reagent Reporting Research Personnel Resistance Resolution Role Sampling Scanning Signal Transduction Slice Small Business Innovation Research Grant Stains Technology Testing Time Tissue Sample Tissues Transcript Tumor Biology Tumor Cell Invasion Tumor Tissue Visualization software base cancer imaging cancer therapy cell type chemotherapy commercialization design disease phenotype effector T cell experimental study improved insight instrument mouse model novel preservation programmed cell death protein 1 prototype scale up single cell analysis specific biomarkers therapy resistant tool transcription factor transcriptome tumor tumor growth tumor microenvironment

项目摘要

SUMMARY Tumor microenvironment (TME) is a key determinant of tumor growth, disease progression and resistance to chemotherapy. There is a diverse repertoire of distinct cell type that are found in the tumor microenvironment, with many that have not been fully enumerated. In addition, little is known about the “geography” of the tumors and the spatial organization of the distinct cell types. There is therefore, an immediate need of transformative tools to enable in-depth understanding of the TME in many forms of malignancies. Spatial Genomics Inc is commercializing seqFISH+ (Eng et al, Nature 2019) which can achieves multiplexing of 10,000 genes at the mRNA level in single cells in tissues with spatial information preserved. Using the seqFISH+ transcriptome profiles, novel cells types in situ and spatial organizations can be directly identified and visualized in tumor tissue slices. These capabilities make seqFISH+ a powerful tool for examining the different cell types and their interactions in the tumor microenvironment. In this SBIR proposal, we will develop an instrument and the associated software and reagents that will allow researchers to perform seqFISH+ in tumor slices. In collaboration with Amgen and Children’s Hospital at LA (CHLA), we will apply seqFISH+ to image the tumor microenvironment in both mouse model systems and human pediatric neuroblastoma samples.

总结