Spatial Genomics for in situ single cell expression analysis in the brain

用于大脑中原位单细胞表达分析的空间基因组学

• 批准号: 10254447
• 负责人: Kirsten Frieda
• 金额: $ 99.45万
• 依托单位: SPATIAL GENOMICS, INC.
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10254447
• 关键词: Address; Adopted; Attention; BRAIN initiative; Brain; Cells; Computer software; Confocal Microscopy; Custom; Data; Data Set; Development; Familiarity; Fluorescent in Situ Hybridization; Future; Gene Expression; Gene Expression Profiling; Genes; Genomics; Human; Image; In Situ; Industry; Laboratories; Liquid substance; Marketing; Messenger RNA; Methods; Microscope; Modeling; Molecular; Mus; Nature; Neurodegenerative Disorders; Neurosciences; Pattern; Phase; Production; Publications; RNA; Research; Research Personnel; Running; Safety; Sampling; Site; Slice; Specific qualifier value; Standardization; Technology; Testing; Tissues; Training; base; brain cell; brain tissue; cell type; commercialization; experience; experimental study; gene panel; instrument; interest; novel; preservation; prototype; screening; single-cell RNA sequencing; touchscreen; transcriptome; transcriptomics; user-friendly

SUMMARY Spatial Genomics, Inc. is commercializing seqFISH technology that can visualize thousands of genes at the mRNA level in single cells with spatial information preserved. Recent advances now make it possible to achieve 10,000 gene multiplexing in single cells directly in tissues (Eng et al., Nature 2019). With this technology, novel cell types can be discovered in situ and spatial organizations can be directly identified and visualized in brain tissue sections.

总结 Spatial Genomics，Inc.正在将seqFISH技术商业化， 基因在mRNA水平在单细胞中的空间信息保存。最近的进展 使得有可能在组织中直接在单细胞中实现10，000个基因的多重化（Eng等， Nature 2019）。通过这项技术，可以在原位和空间发现新的细胞类型， 组织可以在脑组织切片中直接识别和可视化。