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Nanocamera for superresolution and Fluorescence fluctuations

用于超分辨率和荧光波动的纳米相机

基本信息

批准号：
9924235
负责人：
ENRICO GRATTON
金额：
$ 29.34万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2021-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (from the application): In TRD1, we propose three subprojects that will result in substantial additions to our hardware and software. Collectively, these subprojects will enhance techniques and methods developed at the LFD. TRD1 with its three subprojects is not a mere update or maintenance of instrumentation. On the contrary, each of the subprojects is novel and has its own merits and they are necessary for our DBP's and collaborations. Specific Aims: During the last five years, fluorescence microscopy has experienced a revolution due to several factors. These factors include better understanding of the physics of optics for achieving super resolution, availability of new types of sensitive detectors, use of computers as an integral part of the microscope, development of light sheet techniques, multi foci multiphoton excitation and the availability of new fluorescent probes, both synthetically produced (quantum dots) and genetically encoded, that offer better brightness and photochemical stability. Consequently, fluorescence microscopy has evolved into a sophisticated technique. Today, there is a major effort to exploit these new capabilities to address urgent problems in cell biology. Imaging methods have become advanced to the point that single molecule detection inside the cell is feasible. One major challenge is the development of methodologies to extract information from the natural systems where all of the biological actions occur simultaneously. The three subprojects address different needs that are not generally found in current efforts for increasing the microscope performance but rather are targeting specific applications that are important for the LFD. These subprojects form a cohesive plan formulated to achieve new quantitative measurements in microscopy.

描述(来自申请)：在TRD1中，我们提出了三个子项目，这将导致我们的硬件和软件的实质性增加。总体而言，这些次级项目将加强LFD开发的技术和方法。TRD1及其三个次级项目不仅仅是对仪器的更新或维护。相反，每个子项目都是新颖的，都有自己的优点，它们是我们DBP和合作所必需的。具体目标：在过去的五年中，由于几个因素，荧光显微镜经历了一场革命。这些因素包括：更好地理解实现超分辨率的光学物理学，新型灵敏探测器的可用性，计算机作为显微镜不可分割的一部分的使用，光片技术的发展，多焦点多光子激发，以及提供更好亮度和光化学稳定性的合成(量子点)和遗传编码的新型荧光探针的可用性。因此，荧光显微镜已经发展成为一项复杂的技术。今天，人们正在努力利用这些新的能力来解决细胞中的紧急问题生物学。成像方法已经先进到可以在细胞内进行单分子检测的地步。一个主要的挑战是开发从自然系统中提取信息的方法，在自然系统中，所有的生物活动都同时发生。这三个子项目解决了不同的需求，这些需求在当前提高显微镜性能的努力中并不常见，但它们针对的是对LFD重要的特定应用。这些子项目形成了一个连贯的计划，以实现显微镜下的新的定量测量。