PALM (PHOTOACTIVATED LOCALIZATION MICROSCOPY)

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我的项目包括建立硬件和软件来做PALM。我将需要联合收割机的最佳波长的光活化蛋白质，我们有（tdEosFP），研究最合适的组合分色镜和过滤器，以获得最多的信息与相机。同时，我将编写一个程序，在Matlab中，将允许我本地化的光活化分子，并达到超分辨率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. My project consists in setting up both the hardware and the software for doing PALM. I will need to combine the optimal wavelengths for the photoactivatable protein we have (tdEosFP), study the the most suitable combination of dichroic mirrors and filters in order to acquire most informations with the camera. Meanwhile I will write a program in Matlab that will allow me to localize the photoactivated molecules and reach the super-resolution.