Translational Biomarker Analytical Core (TBAC)

转化生物标志物分析核心 (TBAC)

• 批准号: 9918397
• 负责人: BLANDINE B LAFERRERE
• 金额: $ 20.09万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9918397
• 关键词: Amino Acids; Animal Model; Attention; Bioinformatics; Biological Assay; Biological Markers; Blood; C-Peptide; Capital; Catecholamines; Classification; Clinical; Clinical Investigator; Collaborations; Complications of Diabetes Mellitus; Consultations; Data; Data Analyses; Development; Diabetes Mellitus; Diet; Dietary Intervention; Disease; Dyslipidemias; Enzyme-Linked Immunosorbent Assay; Equipment; Experimental Designs; Feeding behaviors; Funding; Genetic; Glucagon; Glucose; Glycerol; Grant; Hepatic; Homeostasis; Hormones; Hydrocortisone; Individual; Institutes; Insulin; Insulin Resistance; Investigation; Ketones; Kinetics; Laboratories; Lipids; Lipoproteins; Measurement; Medical; Metabolic; Metabolic Marker; Metabolism; Methodology; Methods; Molecular Genetics; Monitor; Nonesterified Fatty Acids; Obesity; Pathway Analysis; Pattern; Pharmaceutical Preparations; Phenotype; Plasma; Postdoctoral Fellow; Productivity; Publications; Radioimmunoassay; Regimen; Reproducibility; Research; Research Personnel; Resources; Services; Techniques; Testing; Time; Training; United States National Institutes of Health; Urine; analytical method; base; blood glucose regulation; cost; cytokine; energy balance; glucagon-like peptide 1; glucose output; glucose tolerance; human subject; improved; innovation; instrumentation; insulin secretion; insulin sensitivity; member; metabolomics; new technology; novel; skills; small molecule; stable isotope; translational scientist

The Translational Biomarker Analytical Core (TBAC) Laboratory makes available to DRC investigators analytical techniques to support studies of diabetes mellitus, insulin resistance, intermediary metabolism, and medical consequences of diabetes in human subjects and animal models. The services provided are best based in a Core Laboratory because they require investigator consultation, special instrumentation and methods that are difficult or impracticable to establish in individual laboratories. In addition, this arrangement allows for coordinated attention to reliability and reproducibility of the assays employed. In investigations of glucose homeostasis, insulin secretion, dyslipidemia, hormone action, molecular genetics, or drug effects, it is essential to be able to monitor hormones such as insulin, C-peptide, glucagon, GLP-1, GIP, PYY, cortisol and catecholamines and such metabolites as glucose, FFA, glycerol, ketones, amino acids, and lipids. With the increasing technical capabilities to develop and validate novel disease biomarkers, one of the key functions of the Core is to expeditiously develop and inaugurate these new methods so that the research base can avail itself of these assays. In the past funding cycle, the Core provided ~80,000 assays to 33 DRC members supported by 81 grants. The resultant data have contributed to 17 new NIH grants, and 85 Core-supported publications (69 as primary, 16 as secondary Core). The SPECIFIC AIMS of the TBAC are: 1) To meet the high demand for a dynamic range of bioassays by the research base, enhancing efficiency, quality, and reproducibility, while minimizing costs. 2) To respond to members’ needs by introducing new assays and methods that leverage the expertise of the Core staff. 3) To provide expert advice to investigators and their associates on analytical methods, experimental design, and data interpretation. 4) To train junior investigators and post-doctoral fellows in practical skills in the Core methods and, if appropriate, transfer analytical methods to their own labs. 5) To facilitate access to targeted metabolomics/lipidomics determinations by the Columbia CTSA Biomarker Laboratory. 6) To facilitate access to experts in bioinformatics, kinetic studies and pathway analyses required to appropriately interpret metabolomics/lipidomics determinations and stable isotopes kinetic studies. 7) To facilitate collaborations among the research base, advertise available resources, and develop and/or facilitate investigator access to new technologies, such as MS-based methods to analyze and quantify metabolites. TBAC analytic and consultative capabilities are closely articulated with the other DRC Cores to provide an integrated approach to quantitative metabolic analyses (Fig. 1).

转化生物标志物分析核心 (TBAC) 实验室向 DRC 提供 研究人员分析技术支持糖尿病、胰岛素抵抗、 人类和动物糖尿病的中间代谢和医学后果 模型。所提供的服务最好以核心实验室为基础，因为它们需要调查员 难以或不切实际的咨询、特殊仪器和方法 个别实验室。此外，这种安排允许协调关注可靠性和 所用测定的再现性。在葡萄糖稳态、胰岛素分泌的研究中， 血脂异常、激素作用、分子遗传学或药物作用，必须能够监测 激素，如胰岛素、C 肽、胰高血糖素、GLP-1、GIP、PYY、皮质醇和儿茶酚胺， 诸如葡萄糖、FFA、甘油、酮、氨基酸和脂质等代谢物。随着越来越多 开发和验证新型疾病生物标志物的技术能力，这是该中心的关键职能之一 核心是要尽快开发和开创这些新方法，使研究基地能够 利用这些测定。在过去的资助周期中，核心为 33 个 DRC 提供了约 80,000 次检测 成员获得 81 项赠款的支持。由此产生的数据为 NIH 提供了 17 项新资助， 85 种核心支持的出版物（69 种作为主要核心，16 种作为次要核心）。具体目标 TBAC的目标是： 1) 满足研究基地对动态范围生物测定的高需求， 提高效率、质量和可重复性，同时最大限度地降低成本。 2) 回应会员的意见 通过引入利用核心人员专业知识的新测定和方法来满足需求。 3) 至 为研究人员及其同事提供有关分析方法、实验设计、 和数据解释。 4）培养初级研究员和博士后的实践技能 核心方法，如果合适，将分析方法转移到他们自己的实验室。 5）方便访问 由哥伦比亚 CTSA 生物标志物实验室进行有针对性的代谢组学/脂质组学测定。 6） 为了方便接触生物信息学、动力学研究和途径分析方面的专家， 正确解释代谢组学/脂质组学测定和稳定同位素动力学研究。 7） 促进研究基地之间的合作，宣传可用资源，并开发 和/或促进研究者获得新技术，例如基于 MS 的方法来分析和 量化代谢物。 TBAC 的分析和咨询能力与 其他 DRC 核心提供定量代谢分析的综合方法（图 1）。