Translational Biomarker Analytical Core (TBAC)

转化生物标志物分析核心 (TBAC)

• 批准号: 9918397
• 负责人: BLANDINE B LAFERRERE
• 金额: $ 20.09万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9918397
• 关键词: Amino Acids; Animal Model; Attention; Bioinformatics; Biological Assay; Biological Markers; Blood; C-Peptide; Capital; Catecholamines; Classification; Clinical; Clinical Investigator; Collaborations; Complications of Diabetes Mellitus; Consultations; Data; Data Analyses; Development; Diabetes Mellitus; Diet; Dietary Intervention; Disease; Dyslipidemias; Enzyme-Linked Immunosorbent Assay; Equipment; Experimental Designs; Feeding behaviors; Funding; Genetic; Glucagon; Glucose; Glycerol; Grant; Hepatic; Homeostasis; Hormones; Hydrocortisone; Individual; Institutes; Insulin; Insulin Resistance; Investigation; Ketones; Kinetics; Laboratories; Lipids; Lipoproteins; Measurement; Medical; Metabolic; Metabolic Marker; Metabolism; Methodology; Methods; Molecular Genetics; Monitor; Nonesterified Fatty Acids; Obesity; Pathway Analysis; Pattern; Pharmaceutical Preparations; Phenotype; Plasma; Postdoctoral Fellow; Productivity; Publications; Radioimmunoassay; Regimen; Reproducibility; Research; Research Personnel; Resources; Services; Techniques; Testing; Time; Training; United States National Institutes of Health; Urine; analytical method; base; blood glucose regulation; cost; cytokine; energy balance; glucagon-like peptide 1; glucose output; glucose tolerance; human subject; improved; innovation; instrumentation; insulin secretion; insulin sensitivity; member; metabolomics; new technology; novel; skills; small molecule; stable isotope; translational scientist

The Translational Biomarker Analytical Core (TBAC) Laboratory makes available to DRC investigators analytical techniques to support studies of diabetes mellitus, insulin resistance, intermediary metabolism, and medical consequences of diabetes in human subjects and animal models. The services provided are best based in a Core Laboratory because they require investigator consultation, special instrumentation and methods that are difficult or impracticable to establish in individual laboratories. In addition, this arrangement allows for coordinated attention to reliability and reproducibility of the assays employed. In investigations of glucose homeostasis, insulin secretion, dyslipidemia, hormone action, molecular genetics, or drug effects, it is essential to be able to monitor hormones such as insulin, C-peptide, glucagon, GLP-1, GIP, PYY, cortisol and catecholamines and such metabolites as glucose, FFA, glycerol, ketones, amino acids, and lipids. With the increasing technical capabilities to develop and validate novel disease biomarkers, one of the key functions of the Core is to expeditiously develop and inaugurate these new methods so that the research base can avail itself of these assays. In the past funding cycle, the Core provided ~80,000 assays to 33 DRC members supported by 81 grants. The resultant data have contributed to 17 new NIH grants, and 85 Core-supported publications (69 as primary, 16 as secondary Core). The SPECIFIC AIMS of the TBAC are: 1) To meet the high demand for a dynamic range of bioassays by the research base, enhancing efficiency, quality, and reproducibility, while minimizing costs. 2) To respond to members’ needs by introducing new assays and methods that leverage the expertise of the Core staff. 3) To provide expert advice to investigators and their associates on analytical methods, experimental design, and data interpretation. 4) To train junior investigators and post-doctoral fellows in practical skills in the Core methods and, if appropriate, transfer analytical methods to their own labs. 5) To facilitate access to targeted metabolomics/lipidomics determinations by the Columbia CTSA Biomarker Laboratory. 6) To facilitate access to experts in bioinformatics, kinetic studies and pathway analyses required to appropriately interpret metabolomics/lipidomics determinations and stable isotopes kinetic studies. 7) To facilitate collaborations among the research base, advertise available resources, and develop and/or facilitate investigator access to new technologies, such as MS-based methods to analyze and quantify metabolites. TBAC analytic and consultative capabilities are closely articulated with the other DRC Cores to provide an integrated approach to quantitative metabolic analyses (Fig. 1).

转化生物标志物分析核心（TBAC）实验室可向刚果民主共和国使用 研究人员分析技术支持糖尿病，胰岛素抵抗的研究， 中介代谢以及人类受试者和动物中糖尿病的医学后果 型号。所提供的服务最好基于核心实验室，因为它们需要调查员 咨询，特殊仪器和难以或不切实际的方法 此外，这种安排允许对可靠性和 所采用的测定的可重复性。葡萄糖稳态，胰岛素分泌的研究， 血脂异常，骑马作用，分子遗传学或药物作用，必须能够监测 胰岛素，C肽，胰高血糖素，GLP-1，GIP，PYY，皮质醇和儿茶酚胺等恐怖剂，以及 葡萄糖，FFA，甘油，酮，氨基酸和脂质等代谢产物。随着增长 发展和验证新型疾病生物标志物的技术能力，这是该疾病的关键功能之一 核心是迅速开发和开启这些新方法，以便研究基础可以 这些分析本身可用。在过去的资金周期中，核心向33 drc提供了约80,000个测定法 成员得到81助理的支持。由此产生的数据贡献了17个新的NIH赠款， 和85个由核心支持的出版物（69个主要是次要核心）。具体目标 TBAC的含义是：1）满足研究基础对动态生物测定范围的高需求， 提高效率，质量和可重复性，同时最大程度地减少成本。 2）回应会员 通过介绍利用核心人员专业知识的新测定法和方法的需求。 3）到 向研究人员及其同事提供有关分析方法，实验设计， 和数据解释。 4）培训初级调查人员和博士后研究员的实际技能 核心方法，如果适当，将分析方法转移到了自己的实验室。 5）促进访问 哥伦比亚CTSA生物标志物实验室确定有针对性的代谢组学/脂肪组学。 6） 为了促进获得生物信息学专家的机会，动力学研究和途径分析需要 适当解释代谢组学/脂质组学测定和稳定的同位素动力学研究。 7） 为了促进研究基础之间的合作，宣传可用资源并开发 和/或最喜欢的研究者访问新技术，例如基于MS的方法进行分析和 定量代谢物。 TBAC分析和咨询功能与 其他DRC核心为定量代谢分析提供了综合方法（图1）。