Light-driven control of neurons in vitro and in vivo

该提案的长期目标是开发和测试新的光学技术 神经功能的光化学控制。我们开发的笼子里的神经递质 以前制造的已经被世界各地的许多研究小组广泛使用。一个重要的 我们的工作特点是我们与诺基亚建立了长期的合作关系 生理学家。这些相互作用对有用和重要的笼子的发展至关重要 化合物，因为它是定义探针的生物学问题。在这项提案中，我们寻求 解决光学化学方法领域中的几个主要差距，这些方法用于 神经功能的光化学探测。也就是说：多色光驱动不是 标准技术；笼状化合物事先不是为双光子激发而设计的。 用于“下一代”(光)药理学的光可逆性药物；基因靶向 光开关，亚基特定的探针来控制行为。这项提案的总体目标是 开发新的光化学工具，允许对各种神经元进行光学控制 体外和体内的信号传递过程。

The long term goal of proposal is the development and testing of new optical technology for photochemical control of neuronal function. The caged neurotransmitters developed we have previously made have been widely used by many research groups around the world. An important feature of our work is that we have forged long-term collaborative relationships with noted physiologists. These interactions have been vital the development of useful and important caged compounds as it is the biological problems that define the probes. In this proposal we seek to address several major gaps in the area of optical chemical methods that are used for photochemical probing of neuronal function. Namely: multi-color photoactuation is not a standard technique; caged compounds are not designed for 2-photon excitation a priori. Photoreversible drugs for “next generation” (opto)pharmacology; genetically targeted photoswitchable, subunit specific probes to control behavior. The overall goal of this proposal is to develop new photochemical tools that allow optical control of a wide variety neuronal signaling processes in vitro and in vivo.