Light-driven control of neurons in vitro and in vivo

体外和体内神经元的光驱动控制

• 批准号: 10737708
• 负责人: Graham Ellis-Davies
• 金额: $ 9.75万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-12-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10737708
• 关键词: Address; Area; Award; Behavior Control; Biological; Cell physiology; Chemicals; Color; Development; Goals; In Vitro; Lasers; Light; Membrane; Methods; Neurons; Neurotransmitters; Optical Methods; Optics; Pharmaceutical Preparations; Pharmacology; Photons; Process; Research; Science; Signal Transduction; Techniques; Technology; Testing; Touch sensation; Work; chromophore; forging; in vivo; neurotransmission; next generation; tool; two-photon

The long term goal of proposal is the development and testing of new optical technology for photochemical control of neuronal function. The caged neurotransmitters developed we have previously made have been widely used by many research groups around the world. An important feature of our work is that we have forged long-term collaborative relationships with noted physiologists. These interactions have been vital the development of useful and important caged compounds as it is the biological problems that define the probes. In this proposal we seek to address several major gaps in the area of optical chemical methods that are used for photochemical probing of neuronal function. Namely: multi-color photoactuation is not a standard technique; caged compounds are not designed for 2-photon excitation a priori. Photoreversible drugs for “next generation” (opto)pharmacology; genetically targeted photoswitchable, subunit specific probes to control behavior. The overall goal of this proposal is to develop new photochemical tools that allow optical control of a wide variety neuronal signaling processes in vitro and in vivo.

该提案的长期目标是开发和测试新的光学技术 用于神经元功能的光化学控制。被关在笼子里的神经递质 我们以前做的已经被许多研究小组广泛使用， 世界我们工作的一个重要特点是，我们建立了长期的合作关系， 与著名生理学家的关系。这些互动至关重要， 开发有用和重要的笼状化合物，因为它是生物学问题 来定义探针。在本提案中，我们力求解决该领域的几个主要差距 用于光化学探测神经元的光化学方法 功能即：多色光致动不是标准技术; 化合物不是为先验的双光子激发而设计的。光可逆药物， “下一代”（光）药理学;基因靶向光开关，亚单位 特定的探针来控制行为。该提案的总体目标是开发新的 光化学工具，允许光学控制各种神经元信号 在体外和体内的过程。