Dietary Modulation of Neuroinflammation in Age-Related Memory Disorders

在25年内，美国65岁及以上的人口将翻一番，达到8000万， 从正常认知老化到神经退行性疾病，与衰老相关的认知衰退的流行 包括阿尔茨海默病（AD）。这些疾病损害生活质量和功能状态， 对个人、其家庭、医疗保健系统造成巨大负担，需要阐明机制 并开发新的治疗方法来预防或至少减缓其进展。 植物性食品和饮料用于健康目的有着悠久的历史和良好的记录。 可可豆含有黄烷醇表儿茶素，一种对血压有益的抗氧化剂， 内皮依赖性血管功能、血小板反应性、胰岛素敏感性、血管炎症，以及 循环祖细胞重要的是，黄烷醇具有神经保护作用，抑制氧化应激 炎症和促进神经发生，神经元存活和突触可塑性，所有这些都是 与神经退行性疾病如AD，肌萎缩侧索硬化症（ALS）， 帕金森病（Parkinson's Disease，PD） 来自人类、非人类灵长类动物和啮齿类动物的证据表明，海马体及其 包括AD在内的衰老相关神经退行性疾病的亚区域。我们最近报道，饮食 摄入可可黄烷醇增加海马功能，以fMRI脑血容量（CBV）测量 一项初步的调解分析表明，可可黄烷醇导致哨兵促炎因子的减少， 介质HMGB 1，先天免疫系统的激活剂。反过来，HMGB 1的减少与以下因素有关： 改善海马功能。最近的证据表明HMGB 1与认知能力下降和损伤有关。 HMGB 1与Toll样受体4（TLR 4）结合，触发促炎细胞因子的产生，包括 TNFa通过NFkB依赖性途径。在内毒素血症和手术创伤的啮齿动物模型中，HMGB 1 介导的海马依赖性记忆障碍与脓毒症患者相似， 通过中和HMGB 1消除。 这些数据将HMGB 1与神经退行性损伤机制联系起来，表明其作为神经退行性损伤的潜力。 治疗靶点，这与全身性炎症放大与各种炎症相关的证据一致。 与年龄相关的病理学，包括阿尔茨海默病。它们有力地支持了我们的主要假设可可 黄烷醇通过其对神经炎症的作用，特别是HMGB 1，通过 TLR 4-NFkB-TNFa信号通路。我们建议在146名受试者的随机对照试验中测试该模型。 参与者，年龄50-69岁，接受高或低的每日可可黄烷醇12周。这样的试验有可能 重大临床影响。

Within 25 years, the US population aged 65 and over will double in size to 80 million bringing, with it an epidemic of aging-related cognitive decline, from normal cognitive aging to neurodegenerative disorders including Alzheimer’s Disease (AD). These conditions impair quality of life and functional status, impose an enormous burden on individuals, their families, the healthcare system, and require elucidation of mechanisms and development of new treatments to prevent or at least slow their progression. The use of plant-based food and drink for health purposes has a long and well-documented history. Cocoa beans contain the flavanol epicatechin, an anti-oxidant with beneficial effects on blood pressure, endothelium-dependent vasomotor function, platelet reactivity, insulin sensitivity, vascular inflammation, and circulating progenitor cells. Importantly, flavanols have neuroprotective effects, suppressing oxidative stress and inflammation and promoting neurogenesis, neuronal survival and synaptic plasticity, all of which are relevant to the pathophysiology of neurodegenerative disorders like AD, amyotrophic lateral sclerosis (ALS), and Parkinson’s Disease (PD). Evidence from humans, non-human primates, and rodents points to a role for the hippocampus and its subregions in aging-related neurodegenerative disorders including AD. We recently reported that dietary intake of cocoa flavanols increased hippocampal function, measured as fMRI cerebral blood volume (CBV) and a pilot mediation analysis showed that cocoa flavanols led to a decrease in the sentinel pro-inflammatory mediator HMGB1, an activator of the innate immune system. In turn, this decrease in HMGB1 was linked to improved hippocampal function. Recent evidence implicates HMGB1 in cognitive decline and impairment. HMGB1 binds to Toll-like receptor 4 (TLR4), triggering the production of pro-inflammatory cytokines including TNFa via NFkB-dependent pathways. In rodent models of endotoxemia and surgical trauma, HMGB1 mediated hippocampal-dependent memory impairment similar to that seen in septic patients, an effect eliminated by neutralizing HMGB1. These data mechanistically link HMGB1 to neurodegenerative impairment, suggesting its potential as a therapeutic target, consistent with evidence that amplified systemic inflammation is associated with a variety of age-related pathologies including Alzheimer’s Disease. They strongly support our major hypothesis that cocoa flavanols improve hippocampal function by their effects on neuroinflammation, specifically HMGB1, via a TLR4-NFkB-TNFa signaling pathway. We propose to test this model in a randomized controlled trial of 146 participants, age 50-69, receiving high or low daily cocoa flavanol for 12 weeks. Such a trial has potential for significant clinical impact.