Development of a Scheimpflug imaging equipped OCT system to measure gradient refractive index of the lens in the human eye in vivo

开发配备 Scheimpflug 成像的 OCT 系统，用于测量体内人眼晶状体的梯度折射率

• 批准号: 9979505
• 负责人: Ji Chang He
• 金额: $ 18.87万
• 依托单位: NEW ENGLAND COLLEGE OF OPTOMETRY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9979505
• 关键词: Adult; Age; Aging; Animals; Anterior; Anterior eyeball segment structure; Area; Back; Caliber; Caring; Cataract; Cataract Extraction; Cellular Structures; Clinical; Computer software; Cornea; Crystalline Lens; Dependence; Development; Diagnosis; Eye; Family suidae; Goals; Growth; Health; Human; Image; Laboratories; Life; Light; Measurement; Measures; Methodology; Morphology; Noise; Operative Surgical Procedures; Optical Coherence Tomography; Optics; Presbyopia; Principal Investigator; Process; Property; Proteins; Pupil; Radial; Refractive Indices; Research; Research Personnel; Retina; Role; Scanning; Scientist; Sclera; Shapes; Source; Structure; Surface; System; Testing; Thick; Time; Update; Vision; Water; clinical practice; design; effective intervention; experimental study; fiber cell; imaging modality; improved; in vivo; individual variation; instrument; lens; novel; operation; programs; tomography; young adult

Project Summary/Abstract The crystalline lens of the human eye grows throughout life with continuous change in its size, shape and internal structure. The lifetime growth of the lens generates a special structure inside the lens: the gradient distribution. From the lens surface to the lens center, the gradient distribution is characterized in its fiber cell concentration, water content and protein concentration at the cellular level. Meanwhile, a cellular mechanism is also established inside the lens to maintain the gradient structure and health of the lens. At the functional level, the gradient cellular structure produces a gradient refractive index (GRIN) of the lens. The lens GRIN causes negative spherical aberration and improves optical quality of the eye by compensating positive spherical aberration from the cornea in both relaxed and accommodated eyes. Better understanding of the lens GRIN is important for eye care professionals in the areas of refractive and cataract surgeries to optimize their surgery design and for vision scientists to explore the cellular mechanisms underlying the developments of presbyopia and cataract. However, accessing the lens GRIN in the human eye in vivo has been a challenge to vision researchers for more than a century. Recently, the Principal Investigator (PI) discovered a novel principle to approach this difficult task by combining Scheimpflug imaging (SI) with anterior segment optical coherence tomography (AS-OCT). This proposed project aims to develop a novel ray-tracing SI equipped AS-OCT system to measure the lens GRIN of the human eye in vivo by applying breakthroughs the PI has recently achieved including: (1) discovery of the principle of in vivo measurement of radial lens GRIN by using a combination of a ray-tracing SI and AS-OCT imaging; (2) discovery of the ray-tracing SI method to identify the point at the posterior lens surface from where the measured lights for both SI and AS-OCT systems were reflected. Advantages of the new system include: (1) constructing a ray-tracing SI equipped AS-OCT system which provides a unique instrument to image the anterior segment of the eye and to study the morphological and optical properties of the lens in vivo; (2) for the first time, accurately measuring radial lens GRIN of the human eye in vivo without any assumption about the optical property of the lens; (3) for the first time, accurately assessing radial lens thickness of the human eye in vivo; (4) accurately deriving profiles of the posterior lens surface of the human eye in vivo; and (5) estimating abnormalities of the refractive index in the aging eye or the eye with cataract development in vivo. Successful accomplishment of the goals of this project will eventually allow us to better understand the functional role of the lens GRIN and its underlying cellular mechanisms in normal and defective eyes and potentially to find effective interventions to control presbyopia and cataract development. It will also present a substantial breakthrough in the methodology for diagnosing lens aging in daily eye care and for revolutionizing refractive and accommodation-restoring surgeries in current clinical practice.

项目摘要/摘要 人眼的结晶镜头在整个生命中都随之而来，其大小，形状不断变化 和内部结构。镜头的寿命增长会在镜头内部产生特殊的结构： 梯度分布。从镜头表面到镜头中心，梯度分布的特征在于 纤维细胞浓度，水含量和蛋白质浓度在细胞水平上。同时，一个细胞 还在镜头内建立了机理，以维持晶状体的梯度结构和健康。在 功能水平，梯度细胞结构产生了镜头的梯度折射率（Grin）。镜头 笑会导致负面的畸变，并通过补偿阳性来提高眼睛的光学质量 从角膜中的球形像差放松和容纳的眼睛。更好地理解 镜头笑对于折射和白内障手术领域的眼保健专业人员很重要，以优化 他们的手术设计和视力科学家探索发展发展的细胞机制 长老会和白内障。但是，在体内访问人眼中的镜头笑一直是一个挑战 向视力研究人员拥有一个多世纪。 最近，首席研究员（PI）发现了一个新的原则，可以通过 将scheimpflug成像（SI）与前段光学相干断层扫描（AS-OCT）相结合。这 拟议的项目旨在开发一种新型的射线追踪SI，配备了AS-OCT系统，以测量镜头笑 通过应用突破，PI最近实现了人类眼睛的眼睛，包括：（1）发现 通过使用射线追踪SI和AS-OCT的组合，对径向镜头笑的体内测量原理 成像； （2）发现射线追踪Si方法以识别后镜头表面的点 SI和AS-OCT系统的测量灯都反映了。新系统的优势包括： （1）构建配备了AS-OCT系统的射线追踪SI，该系统提供了独特的仪器来对象 眼前段并研究体内镜头的形态和光学特性； （2） 第一次，准确地测量了体内人眼的径向镜头笑容，没有任何假设 镜头的光学特性； （3）首次准确评估人眼的径向镜头厚度 体内（4）精确得出体内人眼后镜头表面的特征； （5）估计 衰老的眼睛或眼睛的折射率异常在体内发育。成功的 实现该项目的目标将最终使我们能够更好地了解 镜头在正常和有缺陷的眼睛中笑及其潜在的细胞机制，并有可能找到 有效控制长老会和白内障发育的有效干预措施。它也将带来实质性的 在日常眼保健中诊断晶状体衰老的方法的突破和彻底改变折射率的方法 以及当前临床实践中的住宿期间手术。