Design and Evaluation of a Plasmodium vivax ultra-dense peptide array
间日疟原虫超密集肽阵列的设计和评估
基本信息
- 批准号:10189044
- 负责人:
- 金额:$ 19.31万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-03-15 至 2023-02-28
- 项目状态:已结题
- 来源:
- 关键词:AfricaAmino AcidsAnimalsAntibody ResponseAntigensAttentionBiological AssayBiological MarkersBiologyBloodChronicCodeCommunitiesDataDevelopmentEpitopesErythrocyte TransfusionEvaluationFalciparum MalariaFoundationsGenesGenomeGenomicsHumanImmune systemImmunityIndividualInfectionLaboratoriesLifeLife Cycle StagesLiverMalariaMeasuresModelingMorbidity - disease rateParasitesPatientsPeptidesPerformancePlasmodium falciparumPlasmodium vivaxPopulationProtein ArrayProteinsResearchResourcesSamplingSerologySerumSignal TransductionSolidSporozoitesTranscriptVaccinesVivax Malariabasecandidate markerclinically relevantdensitydesigngenome sequencinggenomic datahuman pathogenimmunogenicimprovednonhuman primatenovelpotential biomarkerpreventsingle-cell RNA sequencingtranscriptome sequencingtranscriptomicstransmission processtransmission-blocking vaccinevaccine candidatevaccine development
项目摘要
The malaria parasite Plasmodium vivax threatens half of the world's population. While on-going elimination
efforts are successfully reducing the burden of falciparum malaria worldwide, the situation is much less promising
for P. vivax. This discrepancy is partially due to our poor understanding of the biology of P. vivax that is difficult
to study in the laboratory. Thus, a better understanding of the parasite proteins recognized by our immune system
could guide vaccine development, enable more rigorous assessments of transmission and exposure, and allow
the identification of individuals carrying dormant parasites. Unfortunately, analyses of the seroreactivity of P.
vivax proteins have been limited to, at best, a few hundred proteins, and those typically derive from blood-stage
parasites. We propose here to leverage extensive genomic and transcriptomic data generated by us and others
to generate a comprehensive list of putative protein-coding transcripts expressed throughout the life cycle of the
parasites. We will design and evaluate the seroreactivity of all those proteins using a high-density peptide array
and serum samples that have been extensively characterized. Our studies will provide a comprehensive
perspective on the P. vivax proteins recognized by the host during an infection, and constitute a solid foundation
for identifying vaccine candidates and biomarkers for measuring exposure and transmission, as well as
potentially, markers associated with the presence of dormant parasites in the liver.
间日疟原虫威胁着世界上一半的人口。虽然正在进行的消除
虽然目前的努力成功地减轻了全世界恶性疟疾的负担,但情况却不那么乐观
间日疟原虫这种差异部分是由于我们对间日疟原虫的生物学了解不足,
在实验室里研究。因此,更好地了解我们的免疫系统识别的寄生虫蛋白质
可以指导疫苗的开发,使传播和暴露的更严格的评估,并允许
识别携带休眠寄生虫的个体。不幸的是,对P.
间日疟原虫的蛋白质最多只有几百种,而这些蛋白质通常来源于血液阶段。
寄生虫我们建议利用我们和其他人产生的广泛的基因组和转录组数据
产生一个全面的清单,推定的蛋白质编码转录本表达的整个生命周期,
寄生虫我们将使用高密度肽阵列设计和评估所有这些蛋白质的血清反应性
和已经被广泛鉴定的血清样本我们的研究将提供全面的
间日疟原虫蛋白在感染过程中被宿主识别,并构成了坚实的基础
用于确定候选疫苗和生物标志物,以测量暴露和传播,以及
潜在地,与肝脏中休眠寄生虫的存在相关的标记。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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David Serre其他文献
David Serre的其他文献
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{{ truncateString('David Serre', 18)}}的其他基金
Host and parasite factors influencing P. vivax RBC invasion and asexual development
影响间日疟原虫红细胞侵袭和无性发育的宿主和寄生虫因素
- 批准号:
10641026 - 财政年份:2022
- 资助金额:
$ 19.31万 - 项目类别:
Multi-Omics Characterization of Plasmodium Vivax Hypnozoites
间日疟原虫休眠子的多组学表征
- 批准号:
10565802 - 财政年份:2022
- 资助金额:
$ 19.31万 - 项目类别:
Design and Evaluation of a Plasmodium vivax ultra-dense peptide array
间日疟原虫超密集肽阵列的设计和评估
- 批准号:
10372166 - 财政年份:2021
- 资助金额:
$ 19.31万 - 项目类别:
Rigorous Assessment of P. vivax Relapses and Primaquine Efficacy for Radical Cure
严格评估间日疟原虫复发和伯氨喹根治疗效
- 批准号:
10469663 - 财政年份:2020
- 资助金额:
$ 19.31万 - 项目类别:
Rigorous Assessment of P. vivax Relapses and Primaquine Efficacy for Radical Cure
严格评估间日疟原虫复发和伯氨喹根治疗效
- 批准号:
10219069 - 财政年份:2020
- 资助金额:
$ 19.31万 - 项目类别:
Rigorous Assessment of P. vivax Relapses and Primaquine Efficacy for Radical Cure
严格评估间日疟原虫复发和伯氨喹根治疗效
- 批准号:
10657543 - 财政年份:2020
- 资助金额:
$ 19.31万 - 项目类别:
Host and Parasite Transcriptional Changes Associated with Immunity to Clinical Malaria in Malian Children
与马里儿童临床疟疾免疫相关的宿主和寄生虫转录变化
- 批准号:
9812115 - 财政年份:2019
- 资助金额:
$ 19.31万 - 项目类别:
Genomic Analyses of Plasmodium vivax Responses to Antimalarial Drugs in Cambodia
柬埔寨间日疟原虫对抗疟药物反应的基因组分析
- 批准号:
9254424 - 财政年份:2013
- 资助金额:
$ 19.31万 - 项目类别:
Genomic Analyses of Plasmodium vivax Responses to Antimalarial Drugs in Cambodia
柬埔寨间日疟原虫对抗疟药物反应的基因组分析
- 批准号:
9398175 - 财政年份:2013
- 资助金额:
$ 19.31万 - 项目类别:
Genomic Analyses of Plasmodium vivax Responses to Antimalarial Drugs in Cambodia
柬埔寨间日疟原虫对抗疟药物反应的基因组分析
- 批准号:
8576980 - 财政年份:2013
- 资助金额:
$ 19.31万 - 项目类别:
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