权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

TR&D Project 3: Photoacoustic Detection of Metal Fluxes at the Tissue Level

基本信息

批准号：
10197971
负责人：
Hao F Zhang
金额：
$ 18.21万
依托单位：
MICHIGAN STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10197971
关键词：
3-Dimensional Adopted Animal Model Animals Biological Biological Process Biological Sciences Brain Bypass Cells Coupling Cryopreservation Cryopreserved Tissue Deposition Detection Development Ensure Extinction (Psychology)Fluorescence Fluorescence Microscopy Fluorescent Probes Future Generations Goals Hemoglobin Image Investigation Ions Kidney Label Larva Lasers Measurement Measures Metals Microfluidics Microscopic Microscopy Modality Modernization Molecular Molecular Probes Mus Optics Organ Pathologic Processes Pathway interactions Physiological Processes Preparation Process Research Research Personnel Resolution Resources Roentgen Rays Sampling Slice System Technology Testing Thick Tissue imaging Tissues Ultrasonic wave Ultrasonography Zebrafish absorption base contrast imaging design detection method detection sensitivity detector fluorescence imaging hemodynamics imaging capabilities imaging platform improved iron metabolism microscopic imaging multi-photon nanoimprinting novel optical imaging pressure quantum response second harmonic technology research and development tool vibration

项目摘要

PROJECT SUMMARY – TR&D PROJECT 3 Photoacoustic Detection of Metal Fluxes at the Tissue Level Modern optical microscopic modalities offer the following optical contrasts: optical scattering, autofluorescence (intrinsic fluorescence), molecular probes (extrinsic fluorescence), and nonlinear optical effects. One key contrast, optical absorption, is inaccessible by established microscopic modalities. As a result, bioelement investigation in biological tissue mainly rely on various fluorescence probes. A lasting challenge in designing fluorescence probes is to overcome low quantum yield, where major optical energy deposited to the probes is dissipated via a non-radiative pathway as heat instead of as fluorescent emissions. Measuring nonradiative thermal generation or optical absorption could offer a new way to conduct bioelement imaging and bypass the quantum yield challenge. Imaging optical absorption quantifies ionic concentrations with high accuracy when the molar extinction coefficient is known. Imaging optical absorption permits direct quantitation of certain highly-optically absorbing ions (i.e. Fe in hemoglobin) and will stimulate the development of a new class of molecular probes that focus on high extinction coefficients only, for which the struggle to achieve high fluorescent quantum yield becomes much less critical. In this Technology Research and Development (TR&D) project we will develop a new optical absorption microscopy modality, referred to as optical Micro-Ring Resonator-Based Photoacoustic Microscopy or MRR- PAM, which measures optical absorption using ultrasound. MRR-PAM detects ultrasound waves induced by laser energy absorption and, consequently, heat generation and thermoelastic vibration. Compared with existing photoacoustic microscopy, MRR-PAM improves the ultrasound detection bandwidth and axial resolution by 10-fold and detection sensitivity by 100-fold. MRR-PAM provides a unique tool to enable tissue imaging in all the DBP themes, such as the strongly scattering brain slices, thick tissue slice or whole mouse kidney, whole zebrafish and zebrafish larvae. It will provide a new tool to quantify iron metabolism. MRR-PAM extends the imaging capabilities developed in other TR&D projects to quantify bioelement distributions and fluxes in both fixed and living tissue slices. It offers the possibility to image whole organs and, potentially, living animals. The unique MRR developed in this TR&D project can be broadly disseminated and will likely impact several other biomedical fields beyond bioelement research.

项目总结- tr&d项目