Macromolecular Crystallography

高分子晶体学

• 批准号: 10205111
• 负责人: Jean Jakoncic
• 金额: $ 123.52万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10205111
• 关键词: Area; Automation; Basic Science; Biological; Charge; Communities; Computer software; Confusion; Critiques; Crystallization; Crystallography; Data; Data Analyses; Data Collection; Development; Dimensions; Doctor of Philosophy; Engineering; Ensure; Equipment; Feedback; Funding; Future; Grant; Heel; Helium; Human Resources; In Situ; Individual; International; Investigation; Light; Maintenance; Methods; National Institute of General Medical Sciences; Optics; Peer Review; Performance; Photons; Preventive; Principal Investigator; Process; Research; Research Personnel; Research Support; Resource Development; Resources; Robot; Roentgen Rays; Running; Sampling; Scanning; Schedule; Science; Scientist; Series; Software Tools; Source; Speed; Structural Biologist; Structure; Synchrotrons; Techniques; Technology; Temperature; Time; Training; Training Support; United States National Institutes of Health; Visit; Work; base; beamline; computerized data processing; computing resources; data handling; data quality; density; detector; experience; experimental study; falls; flexibility; frontier; improved; instrument; instrumentation; new technology; operation; programs; quality assurance; ranpirnase; research and development; success

Abstract: Macromolecular Crystallography Core The aim of the macromolecular crystallography (MX) core is to provide access to, and support for the use of, world-class beamlines for MX. There are two MX beamlines supported through this core, the first is the Highly Automated Macromolecular Crystallography beamline (AMX) (17ID-1), a tunable, high flux microbeam where we have placed an emphasis on automation of the experiments. The second beamline, Frontier Microfocusing Macromolecular Crystallography (FMX) (ID17-2) offers a high-flux x-ray beam with dimensions tunable from 1 to 10 micrometers, and the software and hardware to support the use of this resource. The success of the resources depends on access, support during experiments, and training of the users in the use of our beamlines. We assure merit-based access through a peer-reviewed process run by the National Synchrotron Light Source II (NSLS-II). To meet the needs of the community we have developed multiple access types that allow science-driven prioritization of access to the largest number of principal investigators. The review of experiments is performed by the independent Peer Review Panel (PRP), who generate a documented ranking and critique for every project submitted by users for each access mode. In addition, the PRP provide regular feedback on the performance and future direction of the beamlines. We provide support for the users of our resource firstly by interaction to discuss their experimental plan, and secondly by training of the scientists and their co-workers to use the instruments. We achieve reliable operation by applying performance standards for our instrumentation, and by performing scheduled preventive maintenance. Each experiment is assigned a “local contact,” the first point of contact for training and support, who is responsible for liaison before, during, and after each experiment. We have a team of seven PhD-trained crystallographers, and several are cross-trained to operate both AMX and FMX. To aid efficient use of the two beamlines we have developed common controls software for the experiments and common hardware when possible. This provides multiple benefits: three different groups of workers need know only one set of controls – the beamline scientists, the visiting users, and the equipment-support engineers and staff. We have a plan to continue the development of the NSLS-II MX beamlines at the leading edge of the technology available. The developments fall into three areas: the optical components that provide the x-ray beams, the hardware and software for performing diffraction experiments, and continued improvements of our data- analysis software and hardware. Beamline instrumentation developments include deploying a multi-layer monochromator to increase brightness, new sample-delivery formats to increase throughput, and new pipelines to improve data handling. Our instrumentation will be improved to allow for more efficient handling of microcrystals and delivery of samples into the beamline by a variety of methods: injectors, high density micro supports and in-situ examination of samples.

摘要：高分子晶体学核心 大分子晶体学（MX）核心的目的是提供访问，并支持使用， 世界一流的MX光束线。有两个MX光束线支持通过这个核心，第一个是高度 自动高分子晶体学光束线（AMX）（17 ID-1），一种可调谐的高通量微束， 我们强调实验的自动化。第二条光束线，前沿微聚焦 大分子晶体学（FMX）（ID 17 -2）提供高通量X射线束，其尺寸可从1 到10微米，以及支持使用此资源的软件和硬件。 这些资源的成功取决于实验期间的访问、支持和用户使用培训 我们的光束线我们通过由国家教育部运行的同行评审程序， 同步加速器光源II（NSLS-II）。为了满足社区的需要，我们开发了多路访问 这些类型允许以科学为导向，优先考虑最大数量的主要研究人员。的 由独立的同行评审小组（PRP）对实验进行评审，该小组生成记录在案的 对用户提交的每种访问模式的每个项目进行排名和评论。此外，PRP还提供 定期反馈光束线的性能和未来方向。 我们首先通过互动讨论他们的实验计划来为资源用户提供支持，然后 第二，培训科学家及其同事使用这些仪器。我们实现可靠的运行 通过对我们的仪器应用性能标准，并通过执行预定的预防性 上维护每个实验都被分配了一个“当地联系人”，这是培训和支持的第一个联系人， 负责每次实验之前、期间和之后的联络。我们有一个七人的团队， 晶体学家，和几个交叉培训操作AMX和FMX。为了帮助有效利用这两个 我们已经开发了用于实验的通用控制软件和通用硬件， 可能这提供了多个好处：三个不同的工人群体只需要知道一套控制- 光束线科学家、访问用户以及设备支持工程师和工作人员。 我们有一个计划，继续发展的NSLS-II MX光束线在技术的领先地位 available.这些发展分为三个方面：提供x射线束的光学元件， 用于进行衍射实验的硬件和软件，以及我们数据的持续改进- 分析软件和硬件。光束线仪器开发包括部署多层 增加亮度的单色仪，增加产量的新样品输送格式，以及新管道 以改善数据处理。我们的仪器将得到改进，以便更有效地处理 微晶体和样品输送到光束线的各种方法：注射器，高密度微 支持和现场检查样品。