Statistical Methods for Analyzing Birth Defects Cohorts

分析出生缺陷队列的统计方法

• 批准号: 10372041
• 负责人: HONGYU ZHAO
• 金额: $ 16.75万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10372041
• 关键词: Address; Affect; Biological; Biotechnology; Child; Communities; Computer software; Congenital Abnormality; Data; Data Aggregation; Data Set; Defect; Disease; Disease model; Economic Burden; Etiology; Family; Funding; Future; Genes; Genetic; Genetic study; Genome; Genomics; Goals; Guidelines; Heterogeneity; Heterozygote; Immune System Diseases; Individual; Inherited; Joints; Malignant Childhood Neoplasm; Mental disorders; Metabolic; Methods; Mutation; Newborn Infant; Parents; Pathway interactions; Pediatric Research; Performance; Phenotype; Play; Research; Research Personnel; Role; Sample Size; Signal Transduction; Societies; Statistical Methods; Structural Congenital Anomalies; Technology; United States National Institutes of Health; Variant; Work; cohort; congenital heart disorder; data resource; de novo mutation; disorder risk; exome sequencing; family burden; gene discovery; genomic data; health economics; improved; next generation sequencing; novel; phenotypic data; pleiotropism; programs; risk prediction; software development; success; tool

Project Summary Birth defects cause significant health and economic burdens to families and societies globally. In recent years, advances in biotechnologies, such as next-generation sequencing, have helped to identify many disease- causing genes for birth defects and childhood cancers. Although the identified genes only explain a small proportion of the cases, these advancements demonstrate the promise of identifying more birth defect-causing genes from the analysis of sequencing data through powerful statistical methods. The NIH Common Fund established the Gabriella Miller Kids First Pediatric Research Program (Kids First) to “develop a pediatric research data resource populated by genome sequence and phenotype data that will be of high value for the communities of investigators who study the genetics of childhood cancers and/or structural birth defects.” The ultimate goal of this project is to develop, implement, and apply novel statistical methods to improve the power of identifying genes causing birth defects across a number of conditions using data from the Kids First Data Resource Center and to make the developed tools available to the scientific community. This will be accomplished through three specific aims. First, we will develop a statistical framework that can simultaneously consider different disease models – including both de novo mutations and rare inherited variants – to more effectively identify disease-causing genes from whole exome sequencing data. Second, we will develop statistical methods to quantify the degree of shared de novo mutation contributions to different birth defects and also methods that can leverage this shared genetics to identify disease-causing genes. Third, after evaluating the performance of our developed methods, we will implement these methods and apply them to the birth defect cohorts currently available at the Kids First Data Resource Center as well as other data sets that will be added in the future. We will also disseminate the software to the scientific community. In accomplishing our aims, we will contribute new statistical tools to analyze birth defects cohorts as well as make new biological discoveries of genes and pathways for different birth defects.

项目摘要 出生缺陷给全球家庭和社会造成巨大的健康和经济负担。近年来， 生物技术的进步，如下一代测序，帮助识别了许多疾病-- 导致出生缺陷和儿童癌症的基因。尽管已识别的基因只解释了一小部分 在大多数病例中，这些进展表明有希望找到更多导致出生缺陷的原因 通过强大的统计方法对基因测序数据进行分析。美国国立卫生研究院共同基金 建立了加布里埃拉·米勒儿童首个儿科研究计划(儿童优先)，以“发展一种儿科 由基因组序列和表型数据填充的研究数据资源，将对 研究儿童癌症和/或结构性出生缺陷遗传学的研究人员社区。这个 这个项目的最终目标是开发、实施和应用新的统计方法来提高能力 使用来自儿童第一数据的数据识别多种情况下导致出生缺陷的基因 资源中心，并向科学界提供开发的工具。这将是 通过三个具体目标来实现。首先，我们将开发一个统计框架，可以同时 考虑不同的疾病模型--包括从头突变和罕见的遗传变异--到更多 从整个外显子组测序数据中有效识别致病基因。第二，我们将发展 量化共享从头突变对不同出生缺陷贡献程度的统计方法 以及能够利用这种共享的遗传学来识别致病基因的方法。第三，之后 评估我们开发的方法的性能，我们将实现这些方法并将它们应用于 目前可在儿童第一数据资源中心获得的出生缺陷队列以及其他数据集 将在未来添加。我们还将向科学界传播该软件。在完成 我们的目标是，我们将贡献新的统计工具来分析出生缺陷队列，以及做出新的生物学 不同出生缺陷的基因和途径的发现。