Research Project 1

研究项目1

• 批准号: 10207632
• 负责人: Michael Parsons
• 金额: $ 10.88万
• 依托单位: FLORIDA GULF COAST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10207632
• 关键词: Affect; Area; Caribbean region; Cell Density; Cells; Ciguatera Poisoning; Ciguatoxins; Climate; Communities; Complement; Computer Models; Consumption; DNA sequencing; Data; Dinophyceae; Disease; Ecology; Ecosystem; Environment; Environmental Impact; Event; Florida; Food Webs; Frequencies; Gambierdiscus; Geography; Goals; Growth; Habitats; Health protection; Incidence; Industry; Investigation; Island; Laboratory Study; Link; Modeling; Natural experiment; Pathway interactions; Pattern; Physiology; Population; Population Dynamics; Prevalence; Process; Public Health; Research; Research Project Grants; Risk; Sea; Seafood; Seasons; Sentinel; Site; Societies; Surface; Techniques; Temperature; Toxic effect; Toxin; United States Virgin Islands; Water; Work; climate change; climate impact; contaminated seafood; coral; economic impact; environmental change; extreme temperature; harmful algal blooms; innovation; member; models and simulation; resilience; response; spatiotemporal; thermal stress; tool

Ciguatera fish poisoning (CFP), the most common form of finfish-borne disease globally, affecting tens of thousands of people each year, is caused by dinoflagellates in the genus Gambierdiscus. Our goal is to investigate how climate change, particularly warming sea surface temperatures, will influence the geographic extent and population dynamics of toxin-producing Gambierdiscus spp. in the Greater Caribbean Region (GCR), and use these findings to model ciguatoxin fluxes into coral reef food webs under predicted climate change scenarios. This proposal builds on several important findings from our previous work, which includes the determination that toxicity in the GCR is tightly linked to just a few Gambierdiscus species, and that the most toxic species G. silvae may be the “super bug” on Caribbean reefs, responsible for the bulk of toxin entering the food web even at low cell densities. This breakthrough discovery presents a fortuitous opportunity to study the physiology, toxicity, and ecology of this highly toxigenic species, including its temperature tolerances and the manner in which it disperses to and colonizes other regions. The scientific premise of this project is that only through a deep understanding of the autecology of the most toxic species and their direct and indirect responses to climate change (e.g., warming sea surface temperatures and coral reef impacts, respectively), will we (society) be able to properly assess and respond to the impact of climate change on CFP incidence in the GCR. We will use innovative experimental approaches to examine the impacts of temperature, including field investigations of local adaptation within and dispersal between Gambierdiscus sub-populations using RADseq (Restriction site-Associated DNA sequencing) and analyses of the spatio-temporal dynamics of toxin-producing Gambierdiscus spp. In addition to investigating broad-scale geographic patterns, we will use natural temperature differences between closely adjacent sites to explore the effects of temperature extremes and variability on the composition of Gambierdiscus communities. These natural experiments will be complemented by laboratory studies to determine resilience of toxin-producing Gambierdiscus spp. to thermal stress under variable temperature regimes. Together, these data will reveal how temperature-driven partitioning of Gambierdiscus communities operates at local, regional, and seasonal scales. This has major implications with respect to the effects of climate-driven warming on the extent and prevalence of ciguatera toxicity in the GCR. The resulting data, along with that produced in RP2 and RP3, will enable us to further develop and calibrate our ciguatoxin flux model, which is the first-ever computational model of ciguatoxin fluxes in the food web. This modeling effort will push forward the boundaries of predictive capacity for CFP events and public health protection.

雪卡鱼中毒 (CFP) 是全球最常见的鱼类传播疾病，影响数十人 每年有数以千计的人是由甘比尔鞭毛虫属的甲藻引起的。我们的目标是 研究气候变化，特别是海洋表面温度升高，将如何影响地理 产毒素甘比尔迪斯菌的范围和种群动态。在大加勒比地区 （GCR），并利用这些发现来模拟预测气候下雪卡毒素流入珊瑚礁食物网的通量 改变场景。该提案建立在我们之前工作的几个重要发现的基础上，其中包括 确定 GCR 的毒性与少数 Gambierdiscus 物种密切相关，并且 毒性最强的物种 G. silvae 可能是加勒比珊瑚礁上的“超级细菌”，产生了大部分毒素 即使在低细胞密度下也能进入食物网。这一突破性发现提供了一个偶然的机会 研究这种高产毒物种的生理学、毒性和生态学，包括其温度 容忍度及其扩散和殖民其他地区的方式。这样做的科学前提 项目的重点是，只有通过深入了解毒性最强的物种的自生态学及其直接 对气候变化的间接反应（例如，海面温度升高和珊瑚礁影响， 分别），我们（社会）是否能够正确评估和应对气候变化对CFP的影响 GCR 中的发生率。我们将使用创新的实验方法来检查温度的影响， 包括对 Gambierdiscus 亚种群内部的当地适应和传播情况进行实地调查 使用 RADseq（限制性位点相关 DNA 测序）并分析 产生毒素的甘比尔圆盘菌属 (Gambierdiscus spp.)除了调查大范围的地理模式外，我们还将使用 邻近地点之间的自然温差，以探索极端温度的影响 以及 Gambierdiscus 群落组成的变异性。这些自然实验将 并辅之以实验室研究，以确定产毒甘比尔氏菌的恢复能力。热 变化温度条件下的应力。这些数据将共同揭示温度如何驱动 甘比尔迪斯库斯群落的划分在地方、区域和季节尺度上进行。这有重大 气候导致的变暖对雪卡的范围和流行率的影响 GCR 中的毒性。由此产生的数据以及 RP2 和 RP3 中生成的数据将使我们能够进一步 开发和校准我们的雪卡毒素通量模型，这是第一个雪卡毒素通量计算模型 在食物网中。这项建模工作将突破 CFP 事件预测能力的界限 和公共卫生保护。