权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Research Project 3

研究项目3

基本信息

批准号：
10207634
负责人：
Robert W Sobol
金额：
$ 12.98万
依托单位：
FLORIDA GULF COAST UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-30 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10207634
关键词：
Acute Affect Aging Apoptosis Autophagocytosis Awareness Biological Caribbean region Cell Death Cell Survival Cells Chemicals Chronic Ciguatera Poisoning Ciguatoxins Collaborations Communication Complex Cytotoxic agent Cytotoxin DNA Damage DNA Repair Inhibition DNA Repair Pathway Dangerousness Defect Disease Environment Exposure to Functional disorder Gambierdiscus Gene Mutation Genes Genome Genomics Grant Health Homeostasis Human Imaging Device Lead Malignant Neoplasms Metabolic Metabolism Mitochondria Modeling Mutagens Necrosis Nerve Degeneration Neurologic Nuclear Organism Pathogenesis Pathway interactions Phenotype Poisoning Population Population Dynamics Process Recovery Research Research Project Grants Resolution Signal Transduction Source Stress Symptoms Syndrome System Technology Tissues Toxin Translations Work bioaccumulation biomarker development cellular imaging community engagement cytotoxic environmental agent exposed human population foodborne gastrointestinal genome integrity genotoxicity harmful algal blooms in vitro Model mitochondrial dysfunction novel novel marker remediation response screening stem cells tool

项目摘要

Project Summary P01 Grant Title: Greater Caribbean Center for Ciguatera Research Project 3 title: Translation: Human mechanisms of genotoxicity and cellular metabolism Project 3 inter-relates with Projects 1 and 2 by providing details of human cellular response following exposure to the isolated Ciguatoxins. Discoveries herein will be shared with the Project leads and Administrative core to provide a comprehensive information flow to the Community Engagement Core for regular content distribution and awareness to the effected stakeholders. Guided by the modeling of Project 1 to understand Gambierdiscus population dynamics in the region, Project 2 will yield the isolated Gambierdiscus-derived toxins, including details on bioaccumulation throughout the foodweb. The Project 3 team will work closely with the Project 2 lead (Alison Robertson) to clarify and discover genotoxicity mechanisms of CTX exposure, how exposure varies (acute vs chronic) and if exposure impacts secondary exposure to other environmental agents. Finally, Project 3 will evaluate detailed mechanisms of subcellular crosstalk that may reveal novel biomarkers associated with exposure. This will be accomplished, as will be described in detail throughout the Research Plan, using novel, state-of-the-art technologies developed by and optimized in the Sobol lab, including a new genotoxin screening platform (CometChip) that provides greater than ~10,000-fold greater capacity and sensitivity than previous genotoxicity analytical approaches, novel high-resolution cellular imaging tools for Ca2+ signaling, metabolite (ATP, NAD+) quantification, cell death mechanisms, mitochondrial function and genomic signaling response.

项目总结