Driving Biomedical Projects

推动生物医学项目 项目概要/摘要 我们已经确定了13个驱动生物医学项目的国家资源先进的核磁共振 技术BTRR.这些项目由PI领导，他们是NMR技术开发的领导者， 他们是在新的和令人兴奋的生物医学研究应用的前沿。这确保 他们将积极参与技术开发，实验设计和开发数据 收集和分析策略。他们还将提供有关技术的即时和有见地的反馈 性能对于DBP，我们有三个具体目标：1）展示能力并提供评估 用于TR&D1开发的超灵敏冷冻探针技术; 2）展示能力并提供 对TR&D2开发的动态核极化（DNP）技术进行评估; 3）演示 能力，并提供评估的串联连接的混合（SCH）磁铁技术开发的 TR&D3.许多残疾人伙伴将积极参与两个甚至三个技术和发展项目。这种宽 广泛的参与促进了各个项目之间想法和技术的扩散。DBPs 其特点是在开发新技术方面处于NMR领域的前沿， 将其应用于极具挑战性的生物医学研究问题。他们中的许多人拥有最先进的 商业核磁共振技术可在他们的家庭机构，并推动边界的灵敏度 利用NMR脉冲序列、样品制备策略和数据的创造性方法， 处理和分析。他们都敏锐地意识到需要提高能力，特别是通过 实现更高的磁场，开发尽可能高的灵敏度探头，并追求动态 核极化作为正交策略，以提高灵敏度。我们与以下公司有长期合作关系 许多DBP贡献者，作为NHMFL设施的合作者和用户， 富有成效的对话。我们认为，我们收集的DBP是最适合推动发展的， 在NIH感兴趣的五个主要领域的生物医学研究计划中，TR&D的开放： cally disordered proteins，membrane proteins，protein assemblies，enzyme mechanisms and metabolomics. 总的来说，通过TR& D提出的技术发展对于推进 敏感性和解决所需的先锋生物医学问题提出的消毒副产品，并通过 在DBPs的指导下，我们将开创核磁共振技术的下一步。

Driving Biomedical Projects Project Summary/Abstract We have identified thirteen driving biomedical projects for the National Resource for Advanced NMR Technology BTRR. These projects are led by PIs who are leaders in NMR technology development in their own regard and who are at the forefront of new and exciting biomedical research applications. This ensures that they will actively participate in technology development, experimental design, and developing data collection and analysis strategies. They will also provide immediate and insightful feedback on technology performance. For the DBPs we have three specific aims: 1) Demonstrate capabilities and provide evaluation for ultrasensitive cryoprobe technologies developed by TR&D1; 2) Demonstrate capabilities and provide evaluation for dynamic nuclear polarization (DNP) technologies developed by TR&D2; and 3) Demonstrate capabilities and provide evaluation for series connected hybrid (SCH) magnet technologies developed by TR&D3. Many of the DBPs will be actively involved with two or even three of the TR&D projects. This wide ranging engagement fosters the proliferation of ideas and techniques between the various projects. The DBPs are characterized by research that is at the forefront of the NMR field in developing new techniques and applying them to extremely challenging biomedical research problems. Many of them have the most advanced commercial NMR technology available at their home institutions, and are pushing the boundaries of sensitivity and resolution with creative approaches to NMR pulse sequences, sample preparation strategies, and data processing and analysis. They are all keenly aware of the need for improved capabilities, particularly through implementing higher magnetic fields, developing the highest sensitivity probes possible, and pursuing dynamic nuclear polarization as an orthogonal strategy to improve sensitivity. We have long standing relationships with many of the DBP contributors, as collaborators and users of the NHMFL facilities ensuring lively and productive dialogue. We view the collection of DBPs we have assembled as the best suited to push devel- opment of the TR&D's with biomedical research programs in five primary areas of interest to the NIH: intrinsi- cally disordered proteins, membrane proteins, protein assemblies, enzyme mechanisms and metabolomics. Collectively, the technology developments proposed through the TR&Ds are paramount to advancing the sensitivity and resolution needed to address vanguard biomedical questions posed by the DBPs, and through the guidance of the DBPs we will pioneer the next step in NMR technology.