Life Sciences Mass Spectrometry Facility (LSMSF-SR)

生命科学质谱设施共享资源 (LSMSF-SR)： 项目概要 质谱分析在与生命相关的各种原子和分子分析中发挥着关键作用 一般科学和具体的癌症研究。质谱，作为测量中的一个类别 科学，由各种提供定性和定量的技术和方法组成 基于原子和分子质量测量的信息。普渡中心进行的测量 癌症研究 (PCCR) 成员范围从原子种类到大型多单元分析物 生物复合物，并涉及多种化合物类别，其中包括小分子药物、脂质、 核酸、蛋白质。 在过去的几年中，质谱法的应用范围经历了巨大的扩展 几十年来，由于重大技术发展带来了新的和改进的制造方法 离子、测量离子和探测离子。例如，革命性电离方法的例子包括 电喷雾电离，这是液相色谱与质量联用的主要扩展的关键 光谱测定法和基质辅助激光解吸电离，这是离子成像进步的关键。 仪器仪表的主要发展包括飞行时间质谱和傅立叶分析的进步 变换质谱方法，特别是使用称为 OrbitrapTM 的静电离子阱。 新型离子探针已经被引入，例如电子转移解离和离子淌度。总的来说， 这些发展使得生命科学领域的质谱应用出现了名副其实的爆炸式增长。 因此，癌症研究人员寻求使用质谱进行的测量类型需要 越来越多的工具和专业知识。为了应对这一挑战，普渡大学在 2018 年协调了所有生命- 生命科学质谱设施内面向科学的质谱服务，一个共享的 普渡大学癌症研究中心的资源。该设施由 11 名员工和大约 20 名员工组成 支持与 PCCR 成员需求相关的各种测量的质谱仪 解决定性和定量问题。该共享资源的工具和专业知识专门用于 满足 PCCR 成员当今的研究需求以及适应和预测未来的需求。

Life Sciences Mass Spectrometry Facility Shared Resource (LSMSF-SR): Project Summary Mass spectrometry plays key roles across a wide range of atomic and molecular analyses relevant to the life sciences in general and cancer research specifically. Mass spectrometry, as a category within measurement science, is comprised of a variety of technologies and methodologies that provide qualitative and quantitative information based on the measurement of atomic and molecular mass. Measurements made by Purdue Center for Cancer Research (PCCR) members range from analytes as diverse as atomic species, to large multi-unit bio-complexes, and involve a variety of compound classes that include, inter alia, small molecule drugs, lipids, nucleic acids, and proteins. Mass spectrometry has experienced a dramatic expansion in its range of applications over the past several decades due to major technological developments that have led to new and improved approaches to making ions, measuring ions, and probing ions. Examples of revolutionary ionization methods, for example, include electrospray ionization, which has been key to the major expansion of liquid chromatography coupled with mass spectrometry, and matrix-assisted laser desorption-ionization, which has been key to advances in ion imaging. Major developments in instrumentation have included advances in time-of-flight mass spectrometry and Fourier transform mass spectrometry approaches, particularly with the electrostatic ion trap referred to as the OrbitrapTM. Novel ion probes have been introduced, such as electron transfer dissociation and ion mobility. Collectively, these developments have enabled a veritable explosion of mass spectrometry applications in the life sciences. As a result, the types of measurements that cancer researchers seek to make with mass spectrometry requires a growing range of tools and expertise. To address this challenge, in 2018 Purdue coordinated all of the life- sciences-oriented mass spectrometry services within the Life Sciences Mass Spectrometry Facility, a shared resource of the Purdue Center for Cancer Research. This Facility is comprised of 11 staff and roughly two dozen mass spectrometers that support a wide range of measurements relevant to the needs of PCCR members that address both qualitative and quantitative questions. The tools and expertise of this Shared Resource are devoted to providing for today's research needs of PCCR members as well as adapting to and anticipating future needs.