Life Sciences Mass Spectrometry Facility (LSMSF-SR)

生命科学质谱仪共享资源(LSMSF-SR)： 项目摘要 质谱学在与生命相关的广泛的原子和分子分析中发挥着关键作用。 一般的科学，特别是癌症研究。质谱学，作为测量中的一个范畴 科学由各种技术和方法组成，这些技术和方法提供了定性和定量的 基于原子和分子质量测量的信息。普渡中心进行的测量 癌症研究(PCCR)的成员范围从原子物种的各种分析物到大型多单位 生物络合物，涉及各种化合物类别，除其他外，包括小分子药物，脂质， 核酸和蛋白质。 在过去的几年里，质谱仪在其应用范围上经历了戏剧性的扩展。 几十年来，由于重大技术的发展，导致了新的和改进的方法来制造 离子、测量离子和探测离子。例如，革命性的电离方法的例子包括 电喷雾电离，这是液-质联用的主要扩展的关键 以及基质辅助激光解吸-电离，这一直是离子成像进展的关键。 仪器方面的主要进展包括飞行时间质谱学和傅里叶变换技术的进展。 转换质谱学方法，特别是使用被称为OrbitRapTM的静电离子陷阱。 介绍了新型离子探针，如电子转移解离和离子迁移率。总而言之， 这些发展使得质谱学在生命科学中的应用出现了名副其实的爆炸性增长。 因此，癌症研究人员试图用质谱学进行的测量类型需要 越来越多的工具和专业知识。为了应对这一挑战，普渡在2018年协调了所有的生命- 生命科学质谱学设施内面向科学的质谱学服务，共享 普渡癌症研究中心的资源。该设施由11名工作人员和大约24名员工组成 支持与PCCR成员的需求相关的广泛测量的质谱计 同时解决定性和定量的问题。此共享资源的工具和专业知识 为PCCR成员提供今天的研究需求，以及适应和预测未来的需求。

Life Sciences Mass Spectrometry Facility Shared Resource (LSMSF-SR): Project Summary Mass spectrometry plays key roles across a wide range of atomic and molecular analyses relevant to the life sciences in general and cancer research specifically. Mass spectrometry, as a category within measurement science, is comprised of a variety of technologies and methodologies that provide qualitative and quantitative information based on the measurement of atomic and molecular mass. Measurements made by Purdue Center for Cancer Research (PCCR) members range from analytes as diverse as atomic species, to large multi-unit bio-complexes, and involve a variety of compound classes that include, inter alia, small molecule drugs, lipids, nucleic acids, and proteins. Mass spectrometry has experienced a dramatic expansion in its range of applications over the past several decades due to major technological developments that have led to new and improved approaches to making ions, measuring ions, and probing ions. Examples of revolutionary ionization methods, for example, include electrospray ionization, which has been key to the major expansion of liquid chromatography coupled with mass spectrometry, and matrix-assisted laser desorption-ionization, which has been key to advances in ion imaging. Major developments in instrumentation have included advances in time-of-flight mass spectrometry and Fourier transform mass spectrometry approaches, particularly with the electrostatic ion trap referred to as the OrbitrapTM. Novel ion probes have been introduced, such as electron transfer dissociation and ion mobility. Collectively, these developments have enabled a veritable explosion of mass spectrometry applications in the life sciences. As a result, the types of measurements that cancer researchers seek to make with mass spectrometry requires a growing range of tools and expertise. To address this challenge, in 2018 Purdue coordinated all of the life- sciences-oriented mass spectrometry services within the Life Sciences Mass Spectrometry Facility, a shared resource of the Purdue Center for Cancer Research. This Facility is comprised of 11 staff and roughly two dozen mass spectrometers that support a wide range of measurements relevant to the needs of PCCR members that address both qualitative and quantitative questions. The tools and expertise of this Shared Resource are devoted to providing for today's research needs of PCCR members as well as adapting to and anticipating future needs.