Life Sciences Mass Spectrometry Facility (LSMSF-SR)

生命科学质谱设施共享资源（LSMSF-SR）： 项目摘要 质谱在与生命相关的广泛的原子和分子分析中起着关键作用 一般科学和癌症研究。质谱，作为测量中的一个类别 科学，是由各种技术和方法，提供定性和定量的 基于原子和分子质量测量的信息。普渡中心进行的测量 癌症研究（PCCR）成员的范围从原子种类的分析物，到大型多单元 生物复合物，并且涉及多种化合物类别，包括小分子药物，脂质， 核酸和蛋白质。 在过去的几年中，质谱法在其应用范围方面经历了急剧的扩展。 几十年来，由于重大的技术发展，导致了新的和改进的方法， 离子、测量离子和探测离子。例如，革命性的电离方法包括 电喷雾电离，这是液相色谱与质谱联用的主要扩展的关键 光谱法和基质辅助激光解吸电离，这是离子成像进步的关键。 仪器的主要发展包括飞行时间质谱和傅立叶 转换质谱法，特别是与静电离子阱称为OrbitrapTM。 新的离子探针已经被引入，如电子转移解离和离子迁移率。总的来说， 这些发展使得质谱法在生命科学中的应用真正爆炸式增长。 因此，癌症研究人员试图用质谱法进行的测量类型需要 越来越多的工具和专业知识。为了应对这一挑战，普渡大学在2018年协调了所有的生活- 生命科学质谱设施内面向科学的质谱服务， Purdue Center for Cancer Research的相关文章该设施由11名工作人员和大约24名 质谱仪，支持与PCCR成员需求相关的广泛测量， 解决定性和定量问题。该共享资源的工具和专业知识致力于 为PCCR成员提供当今的研究需求，并适应和预测未来的需求。

Life Sciences Mass Spectrometry Facility Shared Resource (LSMSF-SR): Project Summary Mass spectrometry plays key roles across a wide range of atomic and molecular analyses relevant to the life sciences in general and cancer research specifically. Mass spectrometry, as a category within measurement science, is comprised of a variety of technologies and methodologies that provide qualitative and quantitative information based on the measurement of atomic and molecular mass. Measurements made by Purdue Center for Cancer Research (PCCR) members range from analytes as diverse as atomic species, to large multi-unit bio-complexes, and involve a variety of compound classes that include, inter alia, small molecule drugs, lipids, nucleic acids, and proteins. Mass spectrometry has experienced a dramatic expansion in its range of applications over the past several decades due to major technological developments that have led to new and improved approaches to making ions, measuring ions, and probing ions. Examples of revolutionary ionization methods, for example, include electrospray ionization, which has been key to the major expansion of liquid chromatography coupled with mass spectrometry, and matrix-assisted laser desorption-ionization, which has been key to advances in ion imaging. Major developments in instrumentation have included advances in time-of-flight mass spectrometry and Fourier transform mass spectrometry approaches, particularly with the electrostatic ion trap referred to as the OrbitrapTM. Novel ion probes have been introduced, such as electron transfer dissociation and ion mobility. Collectively, these developments have enabled a veritable explosion of mass spectrometry applications in the life sciences. As a result, the types of measurements that cancer researchers seek to make with mass spectrometry requires a growing range of tools and expertise. To address this challenge, in 2018 Purdue coordinated all of the life- sciences-oriented mass spectrometry services within the Life Sciences Mass Spectrometry Facility, a shared resource of the Purdue Center for Cancer Research. This Facility is comprised of 11 staff and roughly two dozen mass spectrometers that support a wide range of measurements relevant to the needs of PCCR members that address both qualitative and quantitative questions. The tools and expertise of this Shared Resource are devoted to providing for today's research needs of PCCR members as well as adapting to and anticipating future needs.