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Hyperspectral Mapping of Cardiac Excitation and Contraction Dynamics

心脏兴奋和收缩动力学的高光谱图

基本信息

批准号：
10225565
负责人：
JUSTUS M ANUMONWO
金额：
$ 15.6万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10225565
关键词：
Ablation Action Potentials Adult Advanced Development Affect Arrhythmia Blood Cardiac Cardiomyopathies Cell physiology Cells Clinic Clinical Clinical Research Complex Coupled Coupling Data Dependence Development Disease Dissociation Dyes Fluorescence Fluorescent Dyes Frequencies Future Generations Goals Gold Health Heart Heart Atrium Heart Diseases Heart failure Image Image Analysis Individual Ion Channel Isoproterenol Label Light Lighting Link Maps Measurement Mechanics Membrane Membrane Potentials Methods Microscopic Modeling Monitor Motion Muscle Cells Myopathy Nature Nifedipine Optics Pattern Pharmacology Phase Physiological Process Property Research Role Series Sheep Short Waves Signal Transduction Spectrum Analysis Structure Surface Technology Testing Tetrodotoxin Time Tissues Toxic effect Visual base blebbistatin cellular imaging clinical application clinically relevant dosage electrical property heart function heart imaging imaging approach imaging modality imaging probe in vivo insight mechanical properties movie novel patch clamp photonics spatiotemporal spectrograph spectroscopic survey transmission process voltage voltage/patch clamp

项目摘要

PROJECT SUMMARY/ABSTRACT This is an R21 proposal for an exploratory research aiming to establish the initial steps toward a novel in vivo mapping technology to transform the clinical study of mechanical and electrical activation waves in the heart. The dynamic mechanical cardiac contraction is two-way coupled to the complex activity associated with dynamic electrical excitations. Disorders in the contraction and excitation dynamical actions, as well as the dissociation in their spatiotemporal coupling, underlie many abnormal conditions, including fatal heart failure and arrhythmias. Our long term goal is to develop a paradigm-shifting approach for studying the highly coupled and dynamic electrical and mechanical activities in the heart in vivo; the central premise of this proposal is that a simultaneous spatiotemporal mapping capable of resolving the mechanical and the electrical activities is critical for understanding mechanisms of health and disease in the heart. Information on the separated dynamical patterns of contraction and excitation waves will enable determining their individual and cooperative role in cardiac disease. Thus, the general objective of this proposal is to demonstrate the feasibility of a new label-free photonics approach for imaging the spatiotemporal patterns of mechanical and electrical associated activities to provide multi-parametric insight into mechanisms of dynamical excitation and contractility. Our developments will be based on movie-format imaging of the heart at short-wave infrared (SWIR; ~1-2.5 µm) light range, which has relatively low blood absorbance and scattering, and which has been proposed recently for both deep tissue and in vivo studies. We propose to use here the sheep heart as a platform model to test the general hypothesis that label-free hyperspectral SWIR light imaging will simultaneously characterize the separated dynamical nature of factors associated with electrical and mechanical cardiac activity. The specific aims in this launching project are as follows: Aim 1: To demonstrate the separability between intrinsic hyperspectral SWIR light imaging of cellular electrical and contraction associated activities. Here we will identify wavelengths in the SWIR range whose absorbance level is specific to the action potential or the contraction in the cell. Aim 2: To determine the differential sensitivity of SWIR light imaging to modulations of the cellular action potential by membrane currents regulators. The correlation between the time-course of the hyperspectral light absorbance and the action potential and contraction will enable a physiological interpretation of the imaging. Aim 3: To demonstrate in blood perfused isolated sheep hearts the relationship between surface reflectance of specific SWIR light bands and propagation of electrical and mechanical associated waves. Here we will optimize the new mapping method for future in vivo clinical application. For example, the foreseen new photonic-based approach will be safe and provide real-time and accurate mapping for guidance of ablation to terminate arrhythmias in the clinic. Overall, accomplishment of the aims will lead to an entirely new, label-free imaging modality for in vivo mapping of simultaneous dynamic electrical and mechanical function of the heart.

项目概要/摘要这是一项探索性研究的 R21 提案，旨在为实现新型体内绘图技术改变了心脏机械和电激活波的临床研究。动态机械心脏收缩与动态心脏收缩相关的复杂活动是双向耦合的。电激发。收缩和兴奋动力作用以及解离障碍在它们的时空耦合中，它们是许多异常状况的基础，包括致命的心力衰竭和心律失常。我们的长期目标是开发一种范式转换方法来研究高度耦合和动态的心脏体内的电和机械活动；该提案的中心前提是同时能够解析机械和电活动的时空映射对于了解心脏健康和疾病的机制。有关分离动态模式的信息收缩波和激励波的研究将能够确定它们在心脏中的个体和协作作用疾病。因此，该提案的总体目标是证明新的无标签光子学的可行性对机械和电气相关活动的时空模式进行成像的方法，以提供对动态激发和收缩机制的多参数洞察。我们的发展将是基于短波红外（SWIR；~1-2.5 µm）光范围内的心脏电影格式成像，相对较低的血液吸收和散射，最近被提出用于深层组织和体内研究。我们建议在这里使用羊心脏作为平台模型来检验以下一般假设：无标记高光谱短波红外光成像将同时表征分离的动力学性质与心脏电活动和机械活动相关的因素。本次启动项目的具体目标是目标 1：证明细胞的固有高光谱 SWIR 光成像之间的可分离性电和收缩相关的活动。在这里，我们将确定 SWIR 范围内的波长，其吸光度水平特定于细胞的动作电位或收缩。目标 2：确定 SWIR 光成像对膜电流对细胞动作电位调节的不同敏感性监管机构。高光谱吸光度随时间变化与作用的相关性电位和收缩将使成像的生理学解释成为可能。目标3：用血液证明灌注离体羊心脏特定短波红外光带的表面反射率与电气和机械相关波的传播。这里我们将优化新的映射方法未来体内临床应用。例如，预见的新的基于光子的方法将是安全且提供实时、准确的绘图，以指导临床消融终止心律失常。全面的，这些目标的实现将带来一种全新的、无标记的体内成像模式心脏的同步动态电和机械功能。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Distinct spectral dynamics of implanted cardiac defibrillator signals in spontaneous termination of polymorphic ventricular tachycardia and fibrillation in patients with electrical and structural diseases.

植入心脏除颤器信号在电和结构疾病患者多形性室性心动过速和颤动自发终止中的独特频谱动力学。

DOI：
10.1093/europace/euac107
发表时间：
2022
期刊：
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology
影响因子：
0
作者：
Calvo,David;Salinas,Lucia;Martínez-Camblor,Pablo;García-Iglesias,Daniel;Alzueta,Javier;Rodríguez,Anibal;Romero,Rafael;Viñolas,Xavier;Fernández-Lozano,Ignacio;Anguera,Ignasi;Villacastín,Julián;Bodegas,Andrés;Fontenla,Adolfo;Jalife
通讯作者：
Jalife

Electrocardiographic Imaging for Atrial Fibrillation: A Perspective From Computer Models and Animal Experiments to Clinical Value.

DOI：
10.3389/fphys.2021.653013
发表时间：
2021
期刊：
Frontiers in physiology
影响因子：
4
作者：
Salinet J;Molero R;Schlindwein FS;Karel J;Rodrigo M;Rojo-Álvarez JL;Berenfeld O;Climent AM;Zenger B;Vanheusden F;Paredes JGS;MacLeod R;Atienza F;Guillem MS;Cluitmans M;Bonizzi P
通讯作者：
Bonizzi P

Panoramic Endocardial Optical Mapping Demonstrates Serial Rotors Acceleration and Increasing Complexity of Activity During Onset of Cholinergic Atrial Fibrillation.

DOI：
10.1161/jaha.121.022300
发表时间：
2021-11-16
期刊：
Journal of the American Heart Association
影响因子：
5.4
作者：
Salvador-Montañés Ó;Ramirez RJ;Takemoto Y;Ennis SR;Garcia-Iglesias D;Wang S;Wolfer PJ;Jiang J;Mironov SV;Pandit SV;Jalife J;Berenfeld O
通讯作者：
Berenfeld O

ELUCIDATING THE ROLE OF THE HIS-PURKINJE SYSTEM DURING LONG QT MEDIATED ARRHYTHMIAS.

阐明 His-Purkinje 系统在长 QT 介导的心律失常中的作用。