Hyperspectral Mapping of Cardiac Excitation and Contraction Dynamics
心脏兴奋和收缩动力学的高光谱图
基本信息
- 批准号:10038100
- 负责人:
- 金额:$ 24.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-08-01 至 2022-07-31
- 项目状态:已结题
- 来源:
- 关键词:AblationAction PotentialsAdultAdvanced DevelopmentAffectArrhythmiaBloodCardiacCardiomyopathiesCell physiologyCellsClinicClinicalClinical ResearchComplexCoupledCouplingDataDependenceDevelopmentDiseaseDissociationDyesFluorescenceFluorescent DyesFrequenciesFutureGenerationsGoalsGoldHealthHeartHeart AtriumHeart DiseasesHeart failureImageImage AnalysisIndividualIon ChannelIsoproterenolLabelLightLightingLinkMapsMeasurementMechanicsMembraneMembrane PotentialsMethodsMicroscopicModelingMonitorMotionMuscle CellsMyopathyNatureNifedipineOpticsPatternPharmacologyPhasePhysiologicalProcessPropertyResearchRoleSeriesSheepShort WavesSignal TransductionSpectrum AnalysisStructureSurfaceTechnologyTestingTetrodotoxinTimeTissuesToxic effectVisualbaseblebbistatincellular imagingclinical applicationclinically relevantdosageelectrical propertyheart functionheart imagingimaging approachimaging modalityimaging probein vivoinsightmechanical propertiesmovienovelpatch clampphotonicsspatiotemporalspectrographspectroscopic surveytransmission processvoltagevoltage/patch clamp
项目摘要
PROJECT SUMMARY/ABSTRACT
This is an R21 proposal for an exploratory research aiming to establish the initial steps toward a novel in vivo
mapping technology to transform the clinical study of mechanical and electrical activation waves in the heart.
The dynamic mechanical cardiac contraction is two-way coupled to the complex activity associated with dynamic
electrical excitations. Disorders in the contraction and excitation dynamical actions, as well as the dissociation
in their spatiotemporal coupling, underlie many abnormal conditions, including fatal heart failure and arrhythmias.
Our long term goal is to develop a paradigm-shifting approach for studying the highly coupled and dynamic
electrical and mechanical activities in the heart in vivo; the central premise of this proposal is that a simultaneous
spatiotemporal mapping capable of resolving the mechanical and the electrical activities is critical for
understanding mechanisms of health and disease in the heart. Information on the separated dynamical patterns
of contraction and excitation waves will enable determining their individual and cooperative role in cardiac
disease. Thus, the general objective of this proposal is to demonstrate the feasibility of a new label-free photonics
approach for imaging the spatiotemporal patterns of mechanical and electrical associated activities to provide
multi-parametric insight into mechanisms of dynamical excitation and contractility. Our developments will be
based on movie-format imaging of the heart at short-wave infrared (SWIR; ~1-2.5 µm) light range, which has
relatively low blood absorbance and scattering, and which has been proposed recently for both deep tissue and
in vivo studies. We propose to use here the sheep heart as a platform model to test the general hypothesis that
label-free hyperspectral SWIR light imaging will simultaneously characterize the separated dynamical nature of
factors associated with electrical and mechanical cardiac activity. The specific aims in this launching project are
as follows: Aim 1: To demonstrate the separability between intrinsic hyperspectral SWIR light imaging of cellular
electrical and contraction associated activities. Here we will identify wavelengths in the SWIR range whose
absorbance level is specific to the action potential or the contraction in the cell. Aim 2: To determine the
differential sensitivity of SWIR light imaging to modulations of the cellular action potential by membrane currents
regulators. The correlation between the time-course of the hyperspectral light absorbance and the action
potential and contraction will enable a physiological interpretation of the imaging. Aim 3: To demonstrate in blood
perfused isolated sheep hearts the relationship between surface reflectance of specific SWIR light bands and
propagation of electrical and mechanical associated waves. Here we will optimize the new mapping method for
future in vivo clinical application. For example, the foreseen new photonic-based approach will be safe and
provide real-time and accurate mapping for guidance of ablation to terminate arrhythmias in the clinic. Overall,
accomplishment of the aims will lead to an entirely new, label-free imaging modality for in vivo mapping of
simultaneous dynamic electrical and mechanical function of the heart.
项目总结/摘要
这是一项探索性研究的R21提案,旨在建立一种新的体内
标测技术将改变心脏机械和电激活波的临床研究。
动态机械心脏收缩是双向耦合到与动态心脏收缩相关的复杂活动。
电激励收缩和兴奋动力学行为的紊乱,以及解离
在它们的时空耦合中,是许多异常情况的基础,包括致命的心力衰竭和心律失常。
我们的长期目标是开发一种范式转变方法来研究高度耦合和动态的
电和机械活动的心脏在体内;这个建议的中心前提是,
能够分辨机械和电活动的时空映射对于
了解心脏健康和疾病的机制。关于分离动力学模式的信息
收缩波和兴奋波的相互作用将能够确定它们在心脏中的单独作用和协同作用。
疾病因此,本提案的总体目标是证明新的无标记光子学的可行性
一种用于对机械和电相关活动的时空模式进行成像的方法,
多参数洞察机制的动态兴奋和收缩性。我们的发展将是
基于短波红外(SWIR; ~1-2.5 µm)光范围内的心脏电影格式成像,
相对低的血液吸收和散射,并且最近已经提出用于深层组织和
体内研究。我们建议在这里使用绵羊心脏作为平台模型来测试一般假设,
无标记的高光谱短波红外光成像将同时表征分离的动力学性质,
与电和机械心脏活动相关的因素。该启动项目的具体目标是
目的1:证明细胞的固有高光谱SWIR光成像之间的分离性,
电和收缩相关活动。在这里,我们将确定SWIR范围内的波长,
吸光度水平对细胞中的动作电位或收缩是特异性的。目标2:确定
SWIR光成像对膜电流对细胞动作电位调制的不同敏感性
监管部门高光谱吸光度的时间过程与作用之间的相关性
电势和收缩将使得能够对成像进行生理学解释。目标3:在血液中进行演示
灌流的离体绵羊心脏的表面反射率之间的关系,
电波和机械波的传播。这里我们将优化新的映射方法,
未来的体内临床应用。例如,可预见的新的基于光子的方法将是安全的,
提供实时和准确的标测,用于指导消融以终止临床心律失常。总的来说,
这些目标的实现将导致一种全新的、无标记的成像模式,用于体内映射,
心脏的同时动态电和机械功能。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JUSTUS M ANUMONWO其他文献
JUSTUS M ANUMONWO的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JUSTUS M ANUMONWO', 18)}}的其他基金
Extracorporeal and Endoscopic SWIR Mapping of Dynamic Muscle Function
动态肌肉功能的体外和内窥镜短波红外映射
- 批准号:
10650439 - 财政年份:2022
- 资助金额:
$ 24.5万 - 项目类别:
Extracorporeal and Endoscopic SWIR Mapping of Dynamic Muscle Function
动态肌肉功能的体外和内窥镜短波红外映射
- 批准号:
10524926 - 财政年份:2022
- 资助金额:
$ 24.5万 - 项目类别:
Hyperspectral Mapping of Cardiac Excitation and Contraction Dynamics
心脏兴奋和收缩动力学的高光谱图
- 批准号:
10225565 - 财政年份:2020
- 资助金额:
$ 24.5万 - 项目类别:
Arrhythmogenicity of human SAP97 Mutations in patient specific iPSC-CMs and Mice
患者特异性 iPSC-CM 和小鼠中人类 SAP97 突变的致心律失常性
- 批准号:
8887736 - 财政年份:2015
- 资助金额:
$ 24.5万 - 项目类别:
Arrhythmogenicity of human SAP97 Mutations in patient specific iPSC-CMs and Mice
患者特异性 iPSC-CM 和小鼠中人类 SAP97 突变的致心律失常性
- 批准号:
8903572 - 财政年份:2014
- 资助金额:
$ 24.5万 - 项目类别:
Molecular Determinants of Function in Kir2.x channels
Kir2.x 通道功能的分子决定因素
- 批准号:
7487735 - 财政年份:2007
- 资助金额:
$ 24.5万 - 项目类别:
Molecular Determinants of Function in Kir2.x channels
Kir2.x 通道功能的分子决定因素
- 批准号:
7924588 - 财政年份:2007
- 资助金额:
$ 24.5万 - 项目类别:
Molecular Determinants of Function in Kir2.x channels
Kir2.x 通道功能的分子决定因素
- 批准号:
7690276 - 财政年份:2007
- 资助金额:
$ 24.5万 - 项目类别:
Molecular Determinants of Function in Kir2.x channels
Kir2.x 通道功能的分子决定因素
- 批准号:
7680972 - 财政年份:2007
- 资助金额:
$ 24.5万 - 项目类别:
Molecular Determinants of Function in Kir2.x channels
Kir2.x 通道功能的分子决定因素
- 批准号:
7320844 - 财政年份:2007
- 资助金额:
$ 24.5万 - 项目类别:
相似海外基金
Kilohertz volumetric imaging of neuronal action potentials in awake behaving mice
清醒行为小鼠神经元动作电位的千赫兹体积成像
- 批准号:
10515267 - 财政年份:2022
- 资助金额:
$ 24.5万 - 项目类别:
Signal processing in horizontal cells of the mammalian retina – coding of visual information by calcium and sodium action potentials
哺乳动物视网膜水平细胞的信号处理 â 通过钙和钠动作电位编码视觉信息
- 批准号:
422915148 - 财政年份:2019
- 资助金额:
$ 24.5万 - 项目类别:
Research Grants
CAREER: Resolving action potentials and high-density neural signals from the surface of the brain
职业:解析来自大脑表面的动作电位和高密度神经信号
- 批准号:
1752274 - 财政年份:2018
- 资助金额:
$ 24.5万 - 项目类别:
Continuing Grant
Development of Nanosheet-Based Wireless Probes for Multi-Simultaneous Monitoring of Action Potentials and Neurotransmitters
开发基于纳米片的无线探针,用于同时监测动作电位和神经递质
- 批准号:
18H03539 - 财政年份:2018
- 资助金额:
$ 24.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Population Imaging of Action Potentials by Novel Two-Photon Microscopes and Genetically Encoded Voltage Indicators
通过新型双光子显微镜和基因编码电压指示器对动作电位进行群体成像
- 批准号:
9588470 - 财政年份:2018
- 资助金额:
$ 24.5万 - 项目类别:
Enhanced quantitative imaging of compound action potentials in multi-fascicular peripheral nerve with fast neural Electrical Impedance Tomography enabled by 3D multi-plane softening bioelectronics
通过 3D 多平面软化生物电子学实现快速神经电阻抗断层扫描,增强多束周围神经复合动作电位的定量成像
- 批准号:
10009724 - 财政年份:2018
- 资助金额:
$ 24.5万 - 项目类别:
Enhanced quantitative imaging of compound action potentials in multi-fascicular peripheral nerve with fast neural Electrical Impedance Tomography enabled by 3D multi-plane softening bioelectronics
通过 3D 多平面软化生物电子学实现快速神经电阻抗断层扫描,增强多束周围神经复合动作电位的定量成像
- 批准号:
10467225 - 财政年份:2018
- 资助金额:
$ 24.5万 - 项目类别:
Fast high-resolution deep photoacoustic tomography of action potentials in brains
大脑动作电位的快速高分辨率深度光声断层扫描
- 批准号:
9423398 - 财政年份:2017
- 资助金额:
$ 24.5万 - 项目类别:
NeuroGrid: a scalable system for large-scale recording of action potentials from the brain surface
NeuroGrid:用于大规模记录大脑表面动作电位的可扩展系统
- 批准号:
9357409 - 财政年份:2016
- 资助金额:
$ 24.5万 - 项目类别:
Noval regulatory mechanisms of axonal action potentials
轴突动作电位的新调节机制
- 批准号:
16K07006 - 财政年份:2016
- 资助金额:
$ 24.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)