Extracorporeal and Endoscopic SWIR Mapping of Dynamic Muscle Function

项目摘要/摘要 心脏、子宫、膀胱和肠道等器官中肌肉细胞的收缩受 并正在影响与电刺激相关的复杂活动。我们的长期目标是开发一种 研究动态行为和机电耦合的范式转换方法 肌肉组织和器官中的特性，更好地将它们与健康和帕金森氏症等疾病联系起来 和心力衰竭。新的短波红外(SWIR；~1-2.5微米)光成像具有相对较低的血液波段 吸光度和散射，最近已被提出用于深层组织和活体成像。这个 该项目的总体目标是开发一种体内、体外和内窥镜下的无标记SWIR 绘制肌肉功能模式图的方法。相应地，记录的无标签多光谱数据集 同时，在优化的SWIR波长将提供新的光谱指纹电子 肌肉组织中的机械作用。我们将以活鼠跳动的心脏为平台，开发 用于多个SWIR波长的高速探测技术，以验证在活体内的一般假设 本征光学成像可以同时刻画不同的动态时空图案 电的和机械的肌肉动作。我们的具体目标是：目标1：证明这一内在 多光谱SWIR光成像技术可用于体外心肌电-机械标测 动力学。我们将使用专门的高速SWIR相机和多光谱交替光源 位于无标记小鼠的体外，以测试体外标测的假说 在两种光学设置下，活体心脏活动的时空模式是可行的：(1a)宽视角物镜 用于远离车身表面定位的组件，以最大限度地扩大观察区域和光线强度 照明和探测。(1B)与身体表面接触的孔探系统，以最少的介质进行成像 异质性和光损失。目的2：演示双模内窥镜SWIR系统的功能 无标记标记法测定心肌电兴奋性和机械收缩能力。我们将在这里测试 假设血液中特定波长的低吸光度和散射允许内窥镜探查 心肌电和机械激活，在以下情况下绘制深层肌肉组织图所需的 大型动物或病人。实现我们的目标将开启一种新的光子标签方法的可能性- 用于活体研究和临床的肌肉组织动态兴奋和收缩动作的自由标测 申请。

PROJECT SUMMARY/ABSTRACT The contraction of muscle cells in organs such as the heart, uterus, urinary bladder and intestine is regulated by and is affecting a complex activity associated with electrical excitation. Our long-term goal is to develop a paradigm-shifting approach for studying the dynamic actions and coupling between electrical and mechanical properties in muscular tissues and organs to better link them to health and diseases such as Parkinson’s disease and heart failure. New short-wave infrared (SWIR; ~1-2.5 µm) light imaging has bands with relatively low blood absorbance and scattering and has been proposed recently for both deep tissue and in vivo imaging. The general objective of this project is to develop an in vivo extracorporeal and endoscopic label-free SWIR approach for mapping patterns of muscular function. Accordingly, label-free multi-spectral datasets recorded simultaneously at optimized SWIR wavelengths will provide novel spectroscopic fingerprints of electro- mechanical actions in muscle tissue. We will use beating hearts of living mice as a platform for developing the technology for high-speed probing at multiple SWIR wavelengths to test the general hypothesis that in vivo intrinsic optical imaging can characterize simultaneously the distinct dynamic spatiotemporal patterns of electrical and mechanical muscular actions. Our Specific Aims are: Aim 1: To demonstrate that intrinsic multi-spectral SWIR light imaging can be used for extracorporeal mapping of cardiac muscle electro-mechanical dynamics. We will use a specialized high-speed SWIR camera and multi-spectral alternating light sources located outside of the body of label-free mice to test the hypothesis that extracorporeal mapping of spatiotemporal patterns of cardiac activity in vivo is feasible in two optical settings: (1a) wide view objective lens assembly for distanced positioning from the body surface to maximize viewing area and intensity of light illumination and probing. (1b) borescope system in contact with the body surface for imaging with minimal media heterogeneity and light loss. Aim 2: To demonstrate functionality of dual-mode endoscopic SWIR system for label-free mapping electrical excitability and mechanical contractility of cardiac muscle. We will test here the hypothesis that low absorbance and scattering of specific wavelengths in blood permits endoscopic probing of the myocardial electrical and mechanical activation, required for mapping deep muscular tissues in cases of large animals or patients. Accomplishing our aims will open the possibility of a new photonic approach for label- free mapping of dynamic excitation and contractile actions in muscular tissues for in vivo research and clinical applications.