Extracorporeal and Endoscopic SWIR Mapping of Dynamic Muscle Function

项目总结/摘要 心脏、子宫、膀胱和肠等器官中的肌肉细胞的收缩由 并且影响了与电兴奋相关的复杂活动。我们的长期目标是发展一个 研究机电耦合动态行为的范式转换方法 肌肉组织和器官的特性，以更好地将它们与健康和疾病（如帕金森病）联系起来 和心力衰竭新的短波红外（SWIR; ~1-2.5 µm）光成像具有相对较低的血 吸收和散射，并且最近已经提出用于深部组织和体内成像。的 本项目的总体目标是开发一种体内体外和内窥镜无标记SWIR 绘制肌肉功能模式的方法。因此，记录的无标记多光谱数据集 同时在优化的SWIR波长将提供新的光谱指纹的电 肌肉组织中的机械作用。我们将使用活老鼠的跳动的心脏作为开发 在多个SWIR波长下进行高速探测的技术，以测试体内 本征光学成像可以同时表征不同的动态时空模式 肌肉的电和机械动作。我们的具体目标是：目标1：证明内在的 多光谱SWIR光成像可用于心肌电机械的体外标测 动力学我们将使用专门的高速短波红外相机和多光谱交替光源 位于无标记小鼠的体外，以检验体外定位的假设。 体内心脏活动的时空模式在两种光学设置中是可行的：（1a）宽视野物镜透镜 用于远离身体表面定位以最大化观察区域和光强度的组件 照明和探测。(1b)与身体表面接触的管道镜系统，用于用最少的介质成像 不均匀性和光损失。目的2：证明双模内窥镜SWIR系统的功能， 无标记标测心肌电兴奋性和机械收缩性。我们将在这里测试 假设血液中特定波长的低吸收和散射允许内窥镜探测 心肌电激活和机械激活，在以下情况下标测深部肌肉组织所需： 大型动物或患者。实现我们的目标将打开一个新的光子方法的标签的可能性- 用于体内研究和临床的肌肉组织中的动态兴奋和收缩动作的自由映射 应用.

PROJECT SUMMARY/ABSTRACT The contraction of muscle cells in organs such as the heart, uterus, urinary bladder and intestine is regulated by and is affecting a complex activity associated with electrical excitation. Our long-term goal is to develop a paradigm-shifting approach for studying the dynamic actions and coupling between electrical and mechanical properties in muscular tissues and organs to better link them to health and diseases such as Parkinson’s disease and heart failure. New short-wave infrared (SWIR; ~1-2.5 µm) light imaging has bands with relatively low blood absorbance and scattering and has been proposed recently for both deep tissue and in vivo imaging. The general objective of this project is to develop an in vivo extracorporeal and endoscopic label-free SWIR approach for mapping patterns of muscular function. Accordingly, label-free multi-spectral datasets recorded simultaneously at optimized SWIR wavelengths will provide novel spectroscopic fingerprints of electro- mechanical actions in muscle tissue. We will use beating hearts of living mice as a platform for developing the technology for high-speed probing at multiple SWIR wavelengths to test the general hypothesis that in vivo intrinsic optical imaging can characterize simultaneously the distinct dynamic spatiotemporal patterns of electrical and mechanical muscular actions. Our Specific Aims are: Aim 1: To demonstrate that intrinsic multi-spectral SWIR light imaging can be used for extracorporeal mapping of cardiac muscle electro-mechanical dynamics. We will use a specialized high-speed SWIR camera and multi-spectral alternating light sources located outside of the body of label-free mice to test the hypothesis that extracorporeal mapping of spatiotemporal patterns of cardiac activity in vivo is feasible in two optical settings: (1a) wide view objective lens assembly for distanced positioning from the body surface to maximize viewing area and intensity of light illumination and probing. (1b) borescope system in contact with the body surface for imaging with minimal media heterogeneity and light loss. Aim 2: To demonstrate functionality of dual-mode endoscopic SWIR system for label-free mapping electrical excitability and mechanical contractility of cardiac muscle. We will test here the hypothesis that low absorbance and scattering of specific wavelengths in blood permits endoscopic probing of the myocardial electrical and mechanical activation, required for mapping deep muscular tissues in cases of large animals or patients. Accomplishing our aims will open the possibility of a new photonic approach for label- free mapping of dynamic excitation and contractile actions in muscular tissues for in vivo research and clinical applications.