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Cell Culture and Engineering Core (CCEC)

细胞培养与工程核心 (CCEC)

基本信息

批准号：
10231259
负责人：
OWEN M WOODWARD
金额：
$ 18.33万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-24 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10231259
关键词：
3-Dimensional Academia Address Affect Alleles Antibodies Autosomal Dominant Polycystic Kidney Biological Assay Cell Culture Techniques Cell Line Cell Nucleus Cell model Cells Cellular Assay Clinical Clinical Data Collaborations Communities Consultations Coupled Custom Cyst Cyst Fluid Data Duct (organ) structure Ductal Epithelial Cell Engineering Ensure Enterobacteria phage P1 Cre recombinase Epithelial Cells Experimental Designs Future Gene Expression Gene Expression Profiling Goals Gold Government Human In Vitro Individual Industry Investigation Kidney Knock-out Knockout Mice Logistics LoxP-flanked allele Maryland Mind Modeling Mus Mutation Organoids Pathway interactions Patients Phenotype Polycystic Kidney Diseases Positioning Attribute Preparation Primary Cell Cultures Procedures Proprotein Convertase 1 Protocols documentation Quality Control Reproducibility Research Research Personnel Resources Services Site Specimen Stretching System Tetracyclines Therapeutic Time Tissues Translations Validation cellular engineering design frontier high standard high throughput screening in vitro Model innovation interest kidney epithelial cell knock-down mutant new technology organizational structure renal epithelium screening therapeutic evaluation transcriptome sequencing treatment response validation studies vector

项目摘要

PROJECT SUMMARY The Maryland Polycystic Kidney Disease Research and Translation Core Center (MPKD-RTCC) Cell Culture and Engineering Core (CCEC) will accelerate progress in PKD research by addressing a major unmet need for well-defined, reliable in vitro cell PKD models with proper controls. With this overarching goal in mind, CCEC developed new in vitro cell and organoid models that we hope will become the “gold-standards” of the field. The CCEC is committed to helping investigators find ideal solutions for all their individual in vitro research needs whether it involves mechanistic exploration, human PKD cell validation studies, or therapeutic screening. To best serve the current and expected future demands of the diverse PKD workforce in academia, government, and industry, the CCEC will provide murine cell models of ADPKD, provide engineered inner medullary collecting duct (IMCD3) cell lines expressing custom PKD1 and PKD2 mutations, provide human kidney ADPKD cells and specimens, provide a cutting edge cystogenesis assay using three-dimensional organoid system, provide high through-put, three- dimensional cyst expansion assay, and provide consultation services to make sure the PKD community can fully utilize the CCEC resources.

项目摘要马里兰州多囊肾病研究和翻译核心中心（MPKD-RTCC）细胞培养和工程核心（CCEC）将加速PKD研究的进展，解决了一个主要的未满足的需求，明确定义的，可靠的体外细胞PKD模型与适当的对照考虑到这一总体目标，CCEC开发了新的体外细胞和类器官我们希望这些模型将成为该领域的“黄金标准”。CCEC致力于帮助研究人员找到理想的解决方案，以满足他们所有的体外研究需求，涉及机制探索、人PKD细胞验证研究或治疗筛选。到最好地服务于学术界多样化PKD劳动力的当前和预期未来需求， CCEC将提供ADPKD的鼠细胞模型，表达定制PKD 1和PKD 2的工程化内髓集合管（IMCD 3）细胞系突变，提供人类肾脏ADPKD细胞和标本，提供前沿使用三维类器官系统的囊生成测定，提供高通量、三维三维囊肿扩张试验，并提供咨询服务，以确保PKD 社区可以充分利用CCEC资源。