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Cell Culture and Engineering Core (CCEC)

细胞培养与工程核心 (CCEC)

基本信息

批准号：
10231259
负责人：
OWEN M WOODWARD
金额：
$ 18.33万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-24 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10231259
关键词：
3-Dimensional Academia Address Affect Alleles Antibodies Autosomal Dominant Polycystic Kidney Biological Assay Cell Culture Techniques Cell Line Cell Nucleus Cell model Cells Cellular Assay Clinical Clinical Data Collaborations Communities Consultations Coupled Custom Cyst Cyst Fluid Data Duct (organ) structure Ductal Epithelial Cell Engineering Ensure Enterobacteria phage P1 Cre recombinase Epithelial Cells Experimental Designs Future Gene Expression Gene Expression Profiling Goals Gold Government Human In Vitro Individual Industry Investigation Kidney Knock-out Knockout Mice Logistics LoxP-flanked allele Maryland Mind Modeling Mus Mutation Organoids Pathway interactions Patients Phenotype Polycystic Kidney Diseases Positioning Attribute Preparation Primary Cell Cultures Procedures Proprotein Convertase 1 Protocols documentation Quality Control Reproducibility Research Research Personnel Resources Services Site Specimen Stretching System Tetracyclines Therapeutic Time Tissues Translations Validation cellular engineering design frontier high standard high throughput screening in vitro Model innovation interest kidney epithelial cell knock-down mutant new technology organizational structure renal epithelium screening therapeutic evaluation transcriptome sequencing treatment response validation studies vector

项目摘要

PROJECT SUMMARY The Maryland Polycystic Kidney Disease Research and Translation Core Center (MPKD-RTCC) Cell Culture and Engineering Core (CCEC) will accelerate progress in PKD research by addressing a major unmet need for well-defined, reliable in vitro cell PKD models with proper controls. With this overarching goal in mind, CCEC developed new in vitro cell and organoid models that we hope will become the “gold-standards” of the field. The CCEC is committed to helping investigators find ideal solutions for all their individual in vitro research needs whether it involves mechanistic exploration, human PKD cell validation studies, or therapeutic screening. To best serve the current and expected future demands of the diverse PKD workforce in academia, government, and industry, the CCEC will provide murine cell models of ADPKD, provide engineered inner medullary collecting duct (IMCD3) cell lines expressing custom PKD1 and PKD2 mutations, provide human kidney ADPKD cells and specimens, provide a cutting edge cystogenesis assay using three-dimensional organoid system, provide high through-put, three- dimensional cyst expansion assay, and provide consultation services to make sure the PKD community can fully utilize the CCEC resources.

项目总结马里兰州多囊肾病研究与翻译核心中心(MPKD-RTCC) 细胞培养和工程核心(CCEC)将通过以下方式加快PKD研究的进展满足对定义明确、可靠的体外细胞PKD模型的主要未满足需求控制。考虑到这一总体目标，CCEC开发了新的体外细胞和有机化合物我们希望这些车型能成为该领域的“黄金标准”。CCEC致力于帮助研究人员为他们所有的个人体外研究需求找到理想的解决方案涉及机械探索、人类PKD细胞验证研究或治疗性筛选。至最好地满足学术界多样化的PKD劳动力的当前和预期的未来需求，政府和业界，CCEC将提供ADPKD的小鼠细胞模型，提供表达定制PKD1和PKD2的工程内髓内集合管(IMCD3)细胞系突变，提供了人类肾脏ADPKD细胞和标本，提供了前沿利用三维有机系统进行的成囊实验，提供了高通量、三维化的三维囊性扩张术，并提供咨询服务，以确保PKD 社区可以充分利用CCEC资源。