Cell Culture and Engineering Core (CCEC)

项目摘要 马里兰州多囊肾病研究和翻译核心中心（MPKD-RTCC） 细胞培养和工程核心（CCEC）将加速PKD研究的进展， 解决了一个主要的未满足的需求，明确定义的，可靠的体外细胞PKD模型与适当的 对照考虑到这一总体目标，CCEC开发了新的体外细胞和类器官 我们希望这些模型将成为该领域的“黄金标准”。CCEC致力于 帮助研究人员找到理想的解决方案，以满足他们所有的体外研究需求， 涉及机制探索、人类PKD细胞验证研究或治疗筛选。到 最好地服务于学术界多样化PKD劳动力的当前和预期未来需求， CCEC将提供ADPKD的鼠细胞模型， 表达定制PKD 1和PKD 2的工程化内髓集合管（IMCD 3）细胞系 突变，提供人类肾脏ADPKD细胞和标本，提供前沿 使用三维类器官系统的囊生成测定，提供高通量、三维 三维囊肿扩张试验，并提供咨询服务，以确保PKD 社区可以充分利用CCEC资源。

PROJECT SUMMARY The Maryland Polycystic Kidney Disease Research and Translation Core Center (MPKD-RTCC) Cell Culture and Engineering Core (CCEC) will accelerate progress in PKD research by addressing a major unmet need for well-defined, reliable in vitro cell PKD models with proper controls. With this overarching goal in mind, CCEC developed new in vitro cell and organoid models that we hope will become the “gold-standards” of the field. The CCEC is committed to helping investigators find ideal solutions for all their individual in vitro research needs whether it involves mechanistic exploration, human PKD cell validation studies, or therapeutic screening. To best serve the current and expected future demands of the diverse PKD workforce in academia, government, and industry, the CCEC will provide murine cell models of ADPKD, provide engineered inner medullary collecting duct (IMCD3) cell lines expressing custom PKD1 and PKD2 mutations, provide human kidney ADPKD cells and specimens, provide a cutting edge cystogenesis assay using three-dimensional organoid system, provide high through-put, three- dimensional cyst expansion assay, and provide consultation services to make sure the PKD community can fully utilize the CCEC resources.