Cell Electrophysiology Core

细胞电生理学核心

• 批准号: 10425002
• 负责人: Jianli Sun
• 金额: $ 18.57万
• 依托单位: DELAWARE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10425002
• 关键词: Area; Award; Behavior; Biological Models; Biomedical Engineering; Biomedical Research; Brain; Cells; Cellular biology; Centers of Research Excellence; Cognition; Communities; Consult; Consultations; Core Facility; Data; Data Analyses; Data Collection; Delaware; Disease; Electrophysiology (science); Ensure; Equipment; Experimental Designs; Faculty; Feeling; Foundations; Funding; Growth; Historically Black Colleges and Universities; Human; Human Resources; Infrastructure; Injury; Institution; Ion Channel; Laboratories; Leadership; Measurable; Memory; Methods; Minority Groups; Molecular; Needs Assessment; Nervous System Physiology; Neurosciences; Neurosciences Research; Phase; Positioning Attribute; Publications; Research; Research Activity; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Research Training; Resources; Scientist; Services; Surveys; Technical Expertise; Techniques; Testing; Thinking; Time; Tissues; Transgenic Model; United States National Institutes of Health; Universities; Work; base; data acquisition; diversity and equity; experience; experimental study; graduate student; innovation; insight; instrumentation; medical schools; multi-electrode arrays; optogenetics; patch clamp; programs; public health relevance; recruit; response; stem cells; symposium; training opportunity; undergraduate student

Cell Electrophysiology Core Summary The primary purpose for this core is to support infrastructure to provide investigators in the neuroscience community in Delaware with access to electrophysiological methods to answer their research questions. Cell electrophysiology provides unique functional assessment and characterization of excitable cells, tissues and ion channels. However, electrophysiological experiments require expensive, specialized equipment, as well as years of time to develop the necessary technical skills for data collection and analysis, and are therefore out of reach for laboratories that are not specialized in that area. This core was established as part of our Phase II COBRE in response to a needs-assessment among the biomedical research community in Delaware that identified a pool of 13 externally-funded researchers throughout the state who wanted to collect electrophysiological data from their model systems, but lacked the means to do so. A survey of graduate students conducted at the annual Delaware Neuroscience Symposium in 2018 found many would like to get some experience with cell electrophysiology to expand the scope of their research projects and broaden their repertoire of techniques. Support from our Delaware Center for Neuroscience Research COBRE and the statewide Delaware IDeA Network of Biomedical Research Excellence (INBRE) enabled the purchase of electrophysiology instrumentation supporting traditional patch clamp, multi-electrode array recordings, and automated patch clamp, ensuring state-of- the-art instrumentation in the core. Our core will provide Delaware neuroscientists access to electrophysiological techniques, experienced collaborators and consulting expertise that will increase the innovation and impact of their research and make them more competitive for external funding. Continued support through a Phase III COBRE award will allow broad dissemination of the availability of our CE Core, its instrumentation and services. By the end of our Phase III award we expect that our CE Core will have measurable downstream impacts including increased numbers of research publications and greater external funding at Delaware State University, an Historically Black university, as well as across the state of Delaware. The increased level of research activity will provide new research and training opportunities for undergraduate and graduate students both within the DSU and other institutions in Delaware.

细胞电生理学核心 摘要 此核心的主要目的是支持基础设施，以提供 特拉华州神经科学界的研究人员可以访问 电生理学方法来回答他们的研究问题。细胞电生理学 为可兴奋的细胞、组织提供独特的功能评估和表征 和离子通道。然而，电生理实验需要昂贵的， 需要专门的设备以及多年的时间来培养必要的数据收集和分析技术技能， 因此，不是该领域的专业实验室是无法接触到的。这一核心是作为 我们的第二阶段Cobre回应了特拉华州生物医学研究社区的需求评估， 确定了全州13名外部资助的研究人员，他们希望收集电生理学 来自他们的模型系统的数据，但缺乏这样做的手段。对一年一度的研究生进行的一项调查 2018年特拉华州神经科学研讨会发现，许多人希望获得一些细胞电生理学的经验 扩大他们的研究项目的范围，拓宽他们的技术套路。 来自我们特拉华州神经科学研究中心Cobre和全州范围内的特拉华州想法的支持 生物医学研究卓越网络(INBRE)使购买电生理仪器成为可能 支持传统膜片钳、多电极阵列记录和自动膜片钳，确保 核心中最先进的仪器。我们的核心将为特拉华州的神经科学家提供电生理学 技术、经验丰富的合作者和咨询专业知识，将提高其 研究并使它们在获得外部资金方面更具竞争力。通过第三阶段Cobre奖继续提供支持 将允许广泛传播我们的CE核心、其仪器和服务的可用性。在我们结束之前 第三阶段获奖我们预计我们的CE核心将产生可衡量的下游影响，包括数量增加 作为一所历史悠久的黑人大学，特拉华州立大学的研究出版物和更多的外部资金 以及整个特拉华州。研究活动水平的提高将提供新的研究和培训 在特斯拉州立大学和特拉华州的其他机构为本科生和研究生提供机会。