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Membrane Protein Stability

膜蛋白稳定性

基本信息

批准号：
10295197
负责人：
Karen G. Fleming
金额：
$ 36万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-01 至 2023-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10295197
关键词：
Address Affect Bacteria Binding Biological Biological Assay Biological Models Biology Catalysis Client Complement Complex Computational algorithm Computer Models Coupling Data Data Reporting Databases Deuterium Disease Disulfides Engineering Environment Enzymes Equilibrium Foundations Fractionation Free Energy Human body Hydrogen Hydrogen Bonding Hydrophobicity Integral Membrane Protein Investigation Kinetics Knowledge Laboratories Lateral Lipid Bilayers Lipids Location Mathematics Measurement Measures Membrane Membrane Proteins Modeling Molecular Mutation Organic solvent product Pharmaceutical Preparations Phospholipids Physical Chemistry Positioning Attribute Protein Engineering Proteins Regulation Research Scaffolding Protein Side Site Solvents Sorting - Cell Movement Specific qualifier value Structure Techniques Testing Therapeutic Thermodynamics Training Vertebral column Water Work base biophysical analysis biophysical tools chemical group combat design driving force experimental study in vivo interfacial microbial microorganism molecular dynamics mutant novel polar environment protein folding protein misfolding protein structure simulation

项目摘要

Project Summary Understanding how proteins fold is a central quest in biology. Studied for over 50 years, investigations of soluble protein folding have proven invaluable for dissecting the molecular basis of a multitude of diseases. By comparison, folding studies of membrane proteins (MPs) lag far behind. The knowledge gained from soluble protein studies cannot simply be transferred to inferences about MPs because their solvents are different. The balance of forces encoding a MP embedded in a lipid bilayer must be distinct from that of soluble proteins in water. Our research efforts contribute to filling this key gap in the understanding the physical chemistry of membrane proteins. We will experimentally determine of energetic forces stabilizing membrane proteins along the steeply changing polarity gradient of the phospholipid bilayer interface. These efforts will be complemented by molecular simulations and mathematic systems modeling. In addition, we aim to incorporate novel techniques to our toolbox to address the energetic importance of backbone hydrogen bonds in transmembrane proteins. Our results have broad ranging impact in the field at large through contributions to information databases used in training computational algorithms and by their incorporation in physically realistic mechanisms for protein folding catalysis by cellular machines.

项目总结

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

E. coli outer membrane and interactions with OmpLA.

DOI：
10.1016/j.bpj.2014.04.024
发表时间：
2014-06
期刊：
Biophysical journal
影响因子：
3.4
作者：
E. Wu;P. Fleming;M. S. Yeom;G. Widmalm;Jeffery B. Klauda;K. Fleming;W. Im
通讯作者：
E. Wu;P. Fleming;M. S. Yeom;G. Widmalm;Jeffery B. Klauda;K. Fleming;W. Im

Revisiting macromolecular hydration with HullRadSAS.

使用 HullRadSAS 重新审视大分子水合。

DOI：
10.1007/s00249-022-01627-8
发表时间：
2023
期刊：
European biophysics journal : EBJ
影响因子：
0
作者：
Fleming,PatrickJ;Correia,JohnJ;Fleming,KarenG
通讯作者：
Fleming,KarenG

Taking deterministic control of membrane protein monomer-dimer measurements.

对膜蛋白单体-二聚体测量进行确定性控制。

DOI：
10.1085/jgp.201711913
发表时间：
2018
期刊：
The Journal of general physiology
影响因子：
0
作者：
Fleming,KarenG
通讯作者：
Fleming,KarenG

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Karen G. Fleming其他文献

A team of chaperones play to win in the bacterial periplasm

一组伴侣蛋白力争在细菌周质中发挥作用。

DOI：
10.1016/j.tibs.2024.03.015
发表时间：
2024-08-01
期刊：
TRENDS IN BIOCHEMICAL SCIENCES
影响因子：
11.000
作者：
Taylor Devlin;Karen G. Fleming
通讯作者：
Karen G. Fleming

Energetics of Dimeric FkpA Binding to a Native Unfolded Membrane Protein Client

DOI：
10.1016/j.bpj.2019.11.2101
发表时间：
2020-02-07
期刊：
Conference abstract
影响因子：
作者：
Michaela A. Roskopf;Dagan C. Marx;Karen G. Fleming
通讯作者：
Karen G. Fleming

FkpA enhances outer membrane protein folding using an extensive interaction interface

DOI：
10.1016/j.bpj.2023.11.1353
发表时间：
2024-02-08
期刊：
Conference abstract
影响因子：
作者：
Taylor A. Devlin;Dagan C. Marx;Anneliese Faustino;Michaela Roskopf;Stephen D. Fried;Karen G. Fleming
通讯作者：
Karen G. Fleming