Functional Analytics Core

项目摘要 近年来，我们能够操纵单个细胞的遗传密码，通过CRISPR/Cas9诱导 诱变和高通量数据的产生已经迅速发展。然而，主要瓶颈 CRISPR/Cas9的使用在技术上仍然具有挑战性，特别是在角质形成细胞中， 比其他类型的细胞更难检测。此外，大多数研究小组的能力， 分析高通量数据并将其纳入有意义的生物学和病理学背景， 有限公司因此，对于CRISPR/Cas9服务的专业知识和能力都有巨大的需求， 以及生物信息学资源来翻译大量的信息，并将其与相关的生物或 疾病的具体情况。为了填补这一关键空白，密歇根大学皮肤生物学和资源为基础的 中心（UM-SBDRC）功能分析核心（FAC）将提供先进的基础设施，资源， 专业知识，并在密歇根大学UM-SBDRC下的培训机会。外交事务委员会正在排队 其使命是提供所需的研究基础设施、共用设施、服务和资源， 对皮肤生物学和疾病进行研究的研究小组。外交事务委员会的目标是 加速，增强和丰富正在进行的基础和转化研究的有效性，并帮助 将外部研究人员引入皮肤生物学领域。这将通过以下两个目标实现： FAC：在目标1中，我们在角质形成细胞中提供CRISPR/Cas9基因组编辑服务， 敲除和敲入角质形成细胞以及突变角质形成细胞系的功能表征， 和目标2，我们提供系统生物学分析，疾病和肿瘤特异性基因， 表达谱分析和训练。这些服务并没有广泛用于皮肤研究 社区，以及通过该核心提供的服务，资源和技术将提供UM- SBDRC成员具有高度创新的方法，将促进和加速正在进行的研究， 有助于吸引新的研究团队进入皮肤研究领域。

PROJECT SUMMARY In recent years our abilities to manipulate the genetic code of individual cells, through CRISPR/Cas9 induced mutagenesis, and generation of high-throughput data have rapidly advanced. However, major bottlenecks remain; the use of CRISPR/Cas9 remains technically challenging, particularly in keratinocytes, which are much more challenging to transfect than other cell-types. Furthermore, the ability of most research groups to analyze high-throughput data and incorporate it into a meaningful biologic and pathologic context remains limited. Therefore, there is an enormous need for expertise and capabilities both for CRISPR/Cas9 services as well as bioinformatic resources to translate the vast information and connect it with relevant biological or disease-specific situation. To fill this critical gap, the University of Michigan Skin Biology and Resource-based Center (UM-SBDRC) Functional Analytics Core (FAC) will provide sophisticated infrastructure, resources, expertise, and training opportunities at the University of Michigan under the UM-SBDRC. The FAC is in line with the NIAMS mission to provide needed research infrastructure, pooled facilities, services, and resources to groups of investigators conducting research on skin biology and diseases. The goal of the FAC is to accelerate, enhance, and enrich the effectiveness of ongoing basic and translational research, and to help bring outside investigators into the field of cutaneous biology. This will be achieved through the two aims of the FAC: In Aim 1 where we provide CRISPR/Cas9 genome editing services in keratinocytes for generation of both knock-out and knock-in keratinocytes as well as functional characterization of mutant keratinocyte lines, and Aim 2, where we provide access to systems biology analyses, disease- and cytokine-specific gene expression profiling, and training. These services are not widely available for the cutaneous research community, and the services, resources, and techniques made available through this Core will provide UM- SBDRC Members with highly innovative approaches that will promote and accelerate ongoing research and help attract new research teams into the fold of cutaneous research.