Toward fast and deep imaging of living tissue with cellular resolution

以细胞分辨率对活体组织进行快速、深度成像

基本信息

  • 批准号:
    10651713
  • 负责人:
  • 金额:
    $ 62.33万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-07-01 至 2026-03-31
  • 项目状态:
    未结题

项目摘要

Abstract An exciting recent development for high spatial resolution deep tissue imaging is long wavelength three- photon fluorescence microscopy (3PM). Since its first demonstration of imaging subcortical structures in the mouse brain, 3PM has driven rapid progress in deep tissue imaging beyond the depth limit of two-photon fluorescence microscopy (2PM). Long-wavelength 3PM is perhaps the most promising new technology for deep imaging within scattering biological tissues, and has potential impacts in a large number of biomedical fields such as neuroscience, immunology, and cancer biology. On the other hand, there are a number of challenges that must be overcome before 3PM can reach its full potential. Because it is a higher-order nonlinear process, three- photon excitation (3PE) is inherently weaker than two-photon excitation (2PE). The weak signal strength of 3PM is particularly problematic for fast imaging of dynamic cellular process. Furthermore, the laser sources for 3PM are not yet optimized for deep tissue penetration, and the complexity and cost of the excitation source is a major barrier for the applications of 3PM in a typical biomedical research lab. Finally, nearly all 3PM applications today are in the brains. Reaching anatomical frontiers is equally possible in other organs with 3PM, but explicit demonstrations of intravital imaging in novel locations are needed to bring deep imaging capability to other biological systems. The research activity of this proposal will directly address the above challenges for in vivo deep tissue 3PM. We will develop a new generation of 3PM that will improve the performance of existing 3PM by two orders of magnitude and enable multi-color deep tissue imaging with a single excitation wavelength. We will demonstrate the unprecedented imaging capabilities with a low-cost, fiber-based laser system, removing a key barrier for the deployment of 3PM in biology labs. Furthermore, by applying our techniques to a wide variety of biological systems, we will create a valuable knowledge base for the applications of 3PM. Our development of the next generation 3PM parallels the development of 2PM, where the concerted development effort in lasers, microscopes, and biological applications in the 1990s made 2PM ubiquitous in biomedical research labs by the early 2000s. Our vision is to make deep, fast 3PM a routine instrument for a wide variety of biomedical applications just as 2PM does in the shallower regions of biological tissues and organs. The successful completion of this program will enable visualization of dynamic process at the sub-cellular level in intact organs and animal models that are completely beyond the reach of any existing imaging techniques.
摘要 高空间分辨率深部组织成像的一个令人兴奋的最新发展是长波长三- 光子荧光显微镜(下午3点)。自从它首次展示了大脑皮质下结构的成像以来 小鼠大脑,下午3点推动深部组织成像突破双光子深度限制取得快速进展 荧光显微镜(下午2:00)。长波长3 PM可能是最有前途的深度探测新技术 在分散的生物组织内进行成像,并在许多生物医学领域具有潜在的影响 如神经科学、免疫学和癌症生物学。另一方面,存在许多挑战, 必须在下午3点充分发挥其潜力之前克服。因为它是一个高阶的非线性过程,三- 光子激发(3PE)本质上弱于双光子激发(2PE)。下午3点的微弱信号强度 对于动态细胞过程的快速成像尤其有问题。此外,下午3点的激光光源 还没有针对深层组织渗透进行优化,而激发源的复杂性和成本是主要的 3 PM在典型生物医学研究实验室应用的障碍。最后,今天几乎所有下午3点的应用程序 都在大脑里。在下午3点到达解剖边界的其他器官也是同样可能的,但明显 需要展示新位置的活体成像,才能将深度成像能力带给其他 生物系统。这项提案的研究活动将直接解决体内的上述挑战 深部组织下午3点我们将开发新一代3 PM,以改进现有3 PM的性能 可提高两个数量级,并可使用单一激发波长进行多色深层组织成像。我们 将使用低成本、基于光纤的激光系统展示前所未有的成像能力,从而消除 生物实验室部署下午3点的关键障碍。此外,通过将我们的技术应用于各种 对于生物系统,我们将为3pm的应用创造一个有价值的知识库。我们的发展 下一代3PM的发展与2PM的发展平行,在激光方面的共同开发努力, 显微镜和20世纪90年代的生物学应用使2 PM在生物医学研究实验室中无处不在 21世纪初。我们的愿景是让深度、快速的3 PM成为各种生物医学的常规仪器 就像2 PM在生物组织和器官的较浅区域所做的那样。成功者 这一计划的完成将使完整器官在亚细胞水平上的动态过程可视化 和动物模型,这些模型完全超出了任何现有成像技术的范围。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Nozomi Nishimura其他文献

Nozomi Nishimura的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Nozomi Nishimura', 18)}}的其他基金

Novel tracers for in vivo studies of waste transport by fluid flows in the brain
用于脑内液体流动废物运输体内研究的新型示踪剂
  • 批准号:
    10732612
  • 财政年份:
    2023
  • 资助金额:
    $ 62.33万
  • 项目类别:
Simultaneous, Cell-Resolved, Bioluminescent Recording From Microcircuits
微电路同步、细胞解析、生物发光记录
  • 批准号:
    10294095
  • 财政年份:
    2021
  • 资助金额:
    $ 62.33万
  • 项目类别:
Simultaneous, Cell-Resolved, Bioluminescent Recording From Microcircuits
微电路同步、细胞解析、生物发光记录
  • 批准号:
    10463819
  • 财政年份:
    2021
  • 资助金额:
    $ 62.33万
  • 项目类别:
Stalled capillary flow: a novel mechanism for hypoperfusion in Alzheimer disease
毛细血管血流停滞:阿尔茨海默病低灌注的新机制
  • 批准号:
    10412670
  • 财政年份:
    2021
  • 资助金额:
    $ 62.33万
  • 项目类别:
Age Compromises Novel Motility and Repair Functions in Stem Cell Niche of Intestinal Crypts
年龄会损害肠隐窝干细胞生态位的新活力和修复功能
  • 批准号:
    9753843
  • 财政年份:
    2018
  • 资助金额:
    $ 62.33万
  • 项目类别:
Diffuse, spectrally-resolved optical strategies for detecting activity of individual neurons from in vivo mammalian brain with GEVIs
使用 GEVI 检测体内哺乳动物大脑中单个神经元活动的漫反射光谱分辨光学策略
  • 批准号:
    9395599
  • 财政年份:
    2017
  • 资助金额:
    $ 62.33万
  • 项目类别:
In vivo tools for analyzing interstitial fluid flow
用于分析间质液流动的体内工具
  • 批准号:
    9751865
  • 财政年份:
    2017
  • 资助金额:
    $ 62.33万
  • 项目类别:
Supplement: Stalled capillary flow affects protein clearance by modulating interstitial fluid flow
补充:毛细血管血流停滞通过调节间质液流动影响蛋白质清除
  • 批准号:
    10617575
  • 财政年份:
    2015
  • 资助金额:
    $ 62.33万
  • 项目类别:
Role of Microvascular Lesions in Alzheimer's Disease
微血管病变在阿尔茨海默病中的作用
  • 批准号:
    8140740
  • 财政年份:
    2010
  • 资助金额:
    $ 62.33万
  • 项目类别:
Role of Microvascular Lesions in Alzheimer's Disease
微血管病变在阿尔茨海默病中的作用
  • 批准号:
    8044027
  • 财政年份:
    2010
  • 资助金额:
    $ 62.33万
  • 项目类别:

相似海外基金

Investigating the Adoption, Actual Usage, and Outcomes of Enterprise Collaboration Systems in Remote Work Settings.
调查远程工作环境中企业协作系统的采用、实际使用和结果。
  • 批准号:
    24K16436
  • 财政年份:
    2024
  • 资助金额:
    $ 62.33万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
WELL-CALF: optimising accuracy for commercial adoption
WELL-CALF:优化商业采用的准确性
  • 批准号:
    10093543
  • 财政年份:
    2024
  • 资助金额:
    $ 62.33万
  • 项目类别:
    Collaborative R&D
Unraveling the Dynamics of International Accounting: Exploring the Impact of IFRS Adoption on Firms' Financial Reporting and Business Strategies
揭示国际会计的动态:探索采用 IFRS 对公司财务报告和业务战略的影响
  • 批准号:
    24K16488
  • 财政年份:
    2024
  • 资助金额:
    $ 62.33万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10107647
  • 财政年份:
    2024
  • 资助金额:
    $ 62.33万
  • 项目类别:
    EU-Funded
Assessing the Coordination of Electric Vehicle Adoption on Urban Energy Transition: A Geospatial Machine Learning Framework
评估电动汽车采用对城市能源转型的协调:地理空间机器学习框架
  • 批准号:
    24K20973
  • 财政年份:
    2024
  • 资助金额:
    $ 62.33万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10106221
  • 财政年份:
    2024
  • 资助金额:
    $ 62.33万
  • 项目类别:
    EU-Funded
De-Adoption Beta-Blockers in patients with stable ischemic heart disease without REduced LV ejection fraction, ongoing Ischemia, or Arrhythmias: a randomized Trial with blinded Endpoints (ABbreviate)
在没有左心室射血分数降低、持续性缺血或心律失常的稳定型缺血性心脏病患者中停用β受体阻滞剂:一项盲法终点随机试验(ABbreviate)
  • 批准号:
    481560
  • 财政年份:
    2023
  • 资助金额:
    $ 62.33万
  • 项目类别:
    Operating Grants
Our focus for this project is accelerating the development and adoption of resource efficient solutions like fashion rental through technological advancement, addressing longer in use and reuse
我们该项目的重点是通过技术进步加快时装租赁等资源高效解决方案的开发和采用,解决更长的使用和重复使用问题
  • 批准号:
    10075502
  • 财政年份:
    2023
  • 资助金额:
    $ 62.33万
  • 项目类别:
    Grant for R&D
Engage2innovate – Enhancing security solution design, adoption and impact through effective engagement and social innovation (E2i)
Engage2innovate — 通过有效参与和社会创新增强安全解决方案的设计、采用和影响 (E2i)
  • 批准号:
    10089082
  • 财政年份:
    2023
  • 资助金额:
    $ 62.33万
  • 项目类别:
    EU-Funded
Collaborative Research: SCIPE: CyberInfrastructure Professionals InnoVating and brOadening the adoption of advanced Technologies (CI PIVOT)
合作研究:SCIPE:网络基础设施专业人员创新和扩大先进技术的采用 (CI PIVOT)
  • 批准号:
    2321091
  • 财政年份:
    2023
  • 资助金额:
    $ 62.33万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了