Core D: Digital Pathology and CyTOF

核心 D：数字病理学和 CyTOF

• 批准号: 10661816
• 负责人: ROBERT A. ANDERS
• 金额: $ 34.22万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10661816
• 关键词: Animals; Bar Codes; Bioinformatics; Biological; Biological Assay; Biopsy Specimen; Cells; Clinical; Collaborations; Color; Communities; Complex; Comprehensive Cancer Center; Cytometry; Data; Data Set; Dedications; Development; Dimensions; Ensure; Equipment; Fibroblasts; Flow Cytometry; Formalin; Generations; Genetic Transcription; Goals; Grant; Histopathology; Housing; Human; Human Resources; Image Analysis; Imaging Techniques; Immune; Immune System Diseases; Immune response; Immunofluorescence Immunologic; Immunohistochemistry; Immunotherapeutic agent; Infrastructure; Institution; Internet; Laboratories; Link; Location; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Maps; Methodology; Methods; Microscope; Molecular Profiling; Mus; Myelogenous; Myeloid Cells; Myeloid-derived suppressor cells; Oncology; Operative Surgical Procedures; Outcome; Pancreatic Ductal Adenocarcinoma; Paraffin Embedding; Pathologist; Pathology; Physicians; Postdoctoral Fellow; Productivity; Program Research Project Grants; Proteins; Proteomics; Quality Control; Research; Research Personnel; Research Project Grants; Resolution; Resources; Rest; Sampling; Scanning; Scientist; Stains; Suppressor-Effector T-Lymphocytes; Suspensions; System; T cell receptor repertoire sequencing; T-Lymphocyte; Technology; Time; Tissue Sample; Tissue Stains; Tissues; Training; Tumor Tissue; Validation; Western Blotting; Work; anticancer research; biomarker panel; cancer immunotherapy; data acquisition; design; digital pathology; empowerment; high dimensionality; liquid crystal polymer; mid-career faculty; multimodality; multiplexed imaging; pancreatic neoplasm; professor; programs; protein biomarkers; protein profiling; quality assurance; response; single cell proteins; single-cell RNA sequencing; stem; success; targeted treatment; technology validation; tool; translational study; tumor; tumor immunology; tumor microenvironment; tumor-immune system interactions

PROJECT SUMMARY The Digital Pathology and Mass Cytometry Core (Core D) will provide state-of-the-art technologies to enable high-parameter single-cell proteomic profiling for all the Research Projects in the proposed P01 Program Project Grant. The core directors are experts in multicolor immune profiling (multispectral immunoflurescence staining) and high-dimensional proteomic profiling (Cytometry by Time-of-Flight; CyTOF) technologies and will work with project investigators to assay human and mouse tissues generated over the five years of the Program Project. Dr. Anders (core co-director) is an Associate Professor of Pathology, is a practicing GI surgical pathologist who co-directs the Tumor Microenvironment (TME) Laboratory in the Bloomberg Kimmel Institute for Cancer Immunotherapy. The core houses approximately 6 million dollars of equipment aimed at multicolor tissue staining and analysis. The major equipment includes 2 Leica Bond automated immunostainers, 6 Akoya automated high capacity multichannel high-resolution scanning microscopes (Vectra and Polaris systems), and 8 data servers linked by 10G and 40G internet connections to the rest of the campus. The TME laboratory is a central resource for multispectral immunofluorescence staining and image analysis for the Johns Hopkins Oncology research community. Dr. Ho (core co-director) is an Assistant Professor of Oncology and Scientific Director of the newly established CyTOF core, housing the third-generation cytometer Helios™, in the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins. Dr. Ho completed his postdoctoral research in the laboratory of Dr. Elizabeth Jaffee with a special emphasis on developing mass cytometry pipelines to profile the immunologic responses. Dr. Ho also co-led the development of analytical pipelines for CyTOF datasets with Dr. Elana Fertig (Bioinformatics and leader for Core C). Both directors, Anders and Ho, have demonstrated a strong track record of productivity utilizing these technologies. The lab spaces and offices dedicated to this Core are located in close proximity within the Cancer Research Building Complex. The goal of the Digital Pathology and Mass Cytometry Core (Core D) is to provide established and validated assay platforms. The Core Directors have already set-up the assay systems and validated specific marker panels. This provides quality assurance and control for assays performed within and across projects. Once acquired, the data will be provided to Core C for analysis and integration, and to Core B for clinical correlations.

项目总结