Core D: Digital Pathology and CyTOF

核心 D：数字病理学和 CyTOF

• 批准号: 10661816
• 负责人: ROBERT A. ANDERS
• 金额: $ 34.22万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10661816
• 关键词: Animals; Bar Codes; Bioinformatics; Biological; Biological Assay; Biopsy Specimen; Cells; Clinical; Collaborations; Color; Communities; Complex; Comprehensive Cancer Center; Cytometry; Data; Data Set; Dedications; Development; Dimensions; Ensure; Equipment; Fibroblasts; Flow Cytometry; Formalin; Generations; Genetic Transcription; Goals; Grant; Histopathology; Housing; Human; Human Resources; Image Analysis; Imaging Techniques; Immune; Immune System Diseases; Immune response; Immunofluorescence Immunologic; Immunohistochemistry; Immunotherapeutic agent; Infrastructure; Institution; Internet; Laboratories; Link; Location; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Maps; Methodology; Methods; Microscope; Molecular Profiling; Mus; Myelogenous; Myeloid Cells; Myeloid-derived suppressor cells; Oncology; Operative Surgical Procedures; Outcome; Pancreatic Ductal Adenocarcinoma; Paraffin Embedding; Pathologist; Pathology; Physicians; Postdoctoral Fellow; Productivity; Program Research Project Grants; Proteins; Proteomics; Quality Control; Research; Research Personnel; Research Project Grants; Resolution; Resources; Rest; Sampling; Scanning; Scientist; Stains; Suppressor-Effector T-Lymphocytes; Suspensions; System; T cell receptor repertoire sequencing; T-Lymphocyte; Technology; Time; Tissue Sample; Tissue Stains; Tissues; Training; Tumor Tissue; Validation; Western Blotting; Work; anticancer research; biomarker panel; cancer immunotherapy; data acquisition; design; digital pathology; empowerment; high dimensionality; liquid crystal polymer; mid-career faculty; multimodality; multiplexed imaging; pancreatic neoplasm; professor; programs; protein biomarkers; protein profiling; quality assurance; response; single cell proteins; single-cell RNA sequencing; stem; success; targeted treatment; technology validation; tool; translational study; tumor; tumor immunology; tumor microenvironment; tumor-immune system interactions

PROJECT SUMMARY The Digital Pathology and Mass Cytometry Core (Core D) will provide state-of-the-art technologies to enable high-parameter single-cell proteomic profiling for all the Research Projects in the proposed P01 Program Project Grant. The core directors are experts in multicolor immune profiling (multispectral immunoflurescence staining) and high-dimensional proteomic profiling (Cytometry by Time-of-Flight; CyTOF) technologies and will work with project investigators to assay human and mouse tissues generated over the five years of the Program Project. Dr. Anders (core co-director) is an Associate Professor of Pathology, is a practicing GI surgical pathologist who co-directs the Tumor Microenvironment (TME) Laboratory in the Bloomberg Kimmel Institute for Cancer Immunotherapy. The core houses approximately 6 million dollars of equipment aimed at multicolor tissue staining and analysis. The major equipment includes 2 Leica Bond automated immunostainers, 6 Akoya automated high capacity multichannel high-resolution scanning microscopes (Vectra and Polaris systems), and 8 data servers linked by 10G and 40G internet connections to the rest of the campus. The TME laboratory is a central resource for multispectral immunofluorescence staining and image analysis for the Johns Hopkins Oncology research community. Dr. Ho (core co-director) is an Assistant Professor of Oncology and Scientific Director of the newly established CyTOF core, housing the third-generation cytometer Helios™, in the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins. Dr. Ho completed his postdoctoral research in the laboratory of Dr. Elizabeth Jaffee with a special emphasis on developing mass cytometry pipelines to profile the immunologic responses. Dr. Ho also co-led the development of analytical pipelines for CyTOF datasets with Dr. Elana Fertig (Bioinformatics and leader for Core C). Both directors, Anders and Ho, have demonstrated a strong track record of productivity utilizing these technologies. The lab spaces and offices dedicated to this Core are located in close proximity within the Cancer Research Building Complex. The goal of the Digital Pathology and Mass Cytometry Core (Core D) is to provide established and validated assay platforms. The Core Directors have already set-up the assay systems and validated specific marker panels. This provides quality assurance and control for assays performed within and across projects. Once acquired, the data will be provided to Core C for analysis and integration, and to Core B for clinical correlations.

项目概要 数字病理学和质谱细胞术核心（核心 D）将提供最先进的技术，以实现 拟议 P01 计划项目中所有研究项目的高参数单细胞蛋白质组分析 授予。核心主任均为多色免疫分析（多光谱免疫荧光染色）专家 和高维蛋白质组分析（飞行时间细胞计数；CyTOF）技术，并将与 项目研究人员对项目五年内产生的人类和小鼠组织进行分析。 Anders 博士（核心联合主任）是病理学副教授，也是一位执业胃肠道外科病理学家， 共同领导彭博金梅尔癌症研究所肿瘤微环境 (TME) 实验室 免疫疗法。核心装有价值约 600 万美元的设备，用于多色组织染色 和分析。主要设备包括Leica Bond自动化免疫染色机2台、Akoya自动化高速染色机6台 容量多通道高分辨率扫描显微镜（Vectra 和 Polaris 系统）和 8 个数据服务器 通过 10G 和 40G 互联网连接与校园的其他部分相连。 TME 实验室是核心资源 用于约翰霍普金斯大学肿瘤学研究的多光谱免疫荧光染色和图像分析 社区。何博士（核心联合主任）是新成立的肿瘤学助理教授兼科学主任 在 Sidney Kimmel 综合中心建立 CyTOF 核心，容纳第三代细胞仪 Helios™ 约翰·霍普金斯大学癌症中心。何博士在Elizabeth博士实验室完成博士后研究 Jaffee 特别强调开发质谱流式细胞仪管道来分析免疫反应。 Ho 博士还与 Elana Fertig 博士共同领导了 CyTOF 数据集分析流程的开发 （生物信息学和核心 C 的领导者）。安德斯和何两位董事都表现出了良好的业绩记录 利用这些技术的生产力。专门用于该核心的实验室空间和办公室位于附近 邻近癌症研究大楼内。 数字病理学和质谱细胞术核心（核心 D）的目标是提供已建立和验证的 分析平台。核心董事已经设置了检测系统并验证了特定标记 面板。这为项目内和跨项目进行的分析提供了质量保证和控制。一次 获得的数据将提供给Core C进行分析和整合，并提供给Core B进行临床 相关性。