Core 2: Biospecimen/Pathology Core

项目摘要 这种共享资源的目的是提供人体组织、生物液体和专家 在专家的指导下为所有项目的研究者进行病理解释 病理学家组织病理学和组织/生物液资源已存在 自1986年以来，并在GI SPORE的支持下扩大。截至今年，资源 包括3156例结直肠癌手术标本，363例结直肠腺瘤手术标本， 结直肠癌肝转移430例，胰腺癌1204例 843例结直肠癌移植瘤，101例胰腺癌移植瘤，6085例 来自结肠直肠癌风险个体的血液样本和2122份血液样本 从胰腺癌患者身上。福尔马林固定石蜡包埋组织，冷冻 绝大多数切除术的材料、临床和结局数据都是可用的， 标本此资源有收藏，包括高品质的速冻和福尔马林固定 来自125名快速尸检参与者的终末期胰腺癌或结直肠癌样本。 目前有911个组织微阵列，其中133个是结肠直肠和胰腺的 肿瘤和疾病。此外，超过16种完全特征化的胰腺癌细胞 已从人肿瘤制备了包括8个家族性胰腺癌细胞系的细胞系 并与研究界广泛分享。采集生物样本并储存起来 根据国家癌症研究所的生物储存库最佳实践指南。 将这些标本分发给SPORE研究人员后， 了过去5年该核心采购额外的异种移植物，新鲜冷冻的结直肠和 胰腺肿瘤和血液及胰液样本，并提供专家病理 向调查人员咨询。标本是在病理学家的监督下收集的 具有结直肠和胰腺肿瘤的专业知识，与临床 这些领域的专家，并与基础研究调查人员密切合作， 最大限度地发挥项目的翻译影响。我们维护一个密码保护的基于网络的 我们组织的追踪系统此基于Web的界面遵循 国家研究理事会的建议，并包括用户认证， 加密、审计跟踪和灾难恢复。建立了一个机制， 向约翰霍普金斯GI内外的研究者分配所需资源 癌症孢子。已与50多个其他机构的调查人员分享了生物样本。

PROJECT SUMMARY The purpose of this shared resource is to provide human tissues, biological fluids, and expert pathologic interpretation for investigators in all of the projects under the direction of expert pathologists. The Histopathology and Tissue/Biologic Fluids Resource has been in existence since 1986 and expanded under the support of the GI SPORE. As of this year, the Resource includes 3156 banked colorectal carcinoma resection specimens, 363 colorectal adenoma samples, 430 hepatic metastases of colorectal carcinoma, 1204 pancreatic carcinoma specimens, 843 xenografts of colorectal carcinoma, 101 pancreatic carcinoma xenografts, 6085 blood specimens from individuals at risk for colorectal carcinoma, and 2122 blood specimens from patients with pancreatic carcinoma. Formalin-fixed paraffin-embedded tissues, frozen materials and clinical and outcome data are available for the vast majority of resection specimens. This resource has collections to include high quality snap frozen and formalin fixed samples of end stage pancreatic or colorectal cancer from 125 rapid autopsy participants. Presently there are 911 tissue microarrays of which 133 are of colorectal and pancreatic neoplasms and diseases. In addition, over 16 fully characterized pancreatic carcinoma cell lines, including 8 familial pancreatic cancer cell lines have been prepared from human tumors and shared broadly with the research community. The biospecimens are harvested and banked in accordance with the National Cancer Institute's Best Practice Guidelines for Biorepositories. Distribution of these specimens to SPORE investigators has resulted in over 120 publications in the past 5 years. This Core procures additional xenografts, fresh frozen colorectal and pancreatic neoplasms and blood and pancreatic juice samples, and provides expert pathologic consultation to investigators. Specimens are collected under the supervision of pathologists with expertise in colorectal and pancreatic neoplasia in close collaboration with clinical specialists in these areas and in similarly close collaboration with basic research investigators to maximize translational impact of the projects. We maintain a password protected web-based tracking system for our tissues in this core. This Web-based interface follows the recommendations of the National Research Council, and includes user authentication, encryption, audit trails, and disaster recovery. A mechanism is in place for prioritization of distribution of requested resources to investigators within and external to the Johns Hopkins GI Cancer SPORE. Biosamples have been shared with investigators at over 50 other institutions.