Core 5: Integrated Support Services

核心5：综合研究支助服务 摘要 综合研究支援服务（ISS）的核心是组织共享的服务，提供给 NEIDL内部的研究社区。这些服务支持BSL-4/ABSL-4研究，以确保每个 研究人员不必在其实验室重新建立基础设施和专门知识， 资助的研究项目。核心将制定时间表和管理成本，以便合理和 对使用这些服务的调查员收取负责任的使用费，以酌情抵消总费用。 所提供的服务随着时间的推移而发展，因此可以根据研究的需要提供新的服务 社区变化。目前，核心服务大致分为动物研究支持服务和 BSL-4实验室服务。动物研究服务包括训练有素的兽医专业人员和兽医 与教师合作进行各种动物模型研究的技术人员。该团队在一个 与动物一起工作时增加安全性的程序数量，特别是那些需要使用 尖锐的。该单位还与调查人员合作，以确保人道地使用动物，并遵守 机构动物护理和使用委员会的要求。在BSL-4实验室服务中， 存在支持BSL-4和ABSL-4研究的单位。其中包括兽医病理学（解剖学和 实验室）服务，负责监督尸检服务，样品的储存和维护， 符合CDC选择代理程序，并负责建立和验证 将组织转移至BSL-2所需的灭活方案。兽医病理学家负责 临床化学病毒学服务负责接收、验证和清点 用于BSL-4研究。这包括生成主库存和工作库存，以及维护 代理商的准确库存。该核心还根据需要开发先进的病毒学工具。病毒基因组 跟踪和单细胞组学资源负责确保从VS到 研究人员的研究，并启动单细胞测序和单病毒组学技术， BSL-4。影像服务部提供了几个平台，研究感染了 BSL-4病原体，包括光片显微镜。许多BSL-4调查人员依赖于 NEIDL比较病理学实验室。免疫学服务提供培训，指导和 在BSL-4足迹内维护先进的免疫学仪器，包括流式细胞术。 高通量筛选已开始运作，包括病毒感染的自动视觉筛选 用小分子抑制剂处理的细胞，并且可以适应任何病毒或细胞靶标。将诊所与 NEIDL计划包括一名临床医生，其职责是将临床结果与研究联系起来， 在NEIDL中进行（用于比较目的），以及与临床项目合作，以获得 用于研究的临床样本。

Core 5: Integrated Research Support Services Abstract The Integrated Research Support Services (ISS) Core serves to organize shared services that are provided to the research community within the NEIDL. These services support BSL-4/ABSL-4 studies to ensure that each investigator does not have to recreate infrastructure and expertise in their laboratories in order to carry out their funded research programs. The core will develop schedules and manage costs so that reasonable and responsible user fees are charged to investigators using these services to offset overall costs, as appropriate. The services offered evolve over time such that new services can be offered as the needs of the research community changes. Currently, core is broadly separated into the Animal Research Support Services, and the BSL-4 Laboratory Services. Animal Research Services includes trained veterinary professionals and veterinary technicians who collaborate with faculty to carry out studies in various animal models. The team is trained on a number of procedures to increase safety when working with animals, especially those that require the use of sharps. This unit also works with investigators to ensure the humane use of animals and to comply with institutional animal care and use committee requirements. Within the BSL-4 Laboratory Services, a number of units exist to support the BSL-4 and ABSL-4 studies. These include Veterinary Pathology (anatomic and laboratory) Service which oversees the necropsy services, storage and maintenance of the samples in compliance with the CDC select agent program, and is responsible for establishing and validating the inactivation protocols required to move tissues to the BSL-2. The Veterinary Pathologist is responsible for clinical chemistry. Virology Services is responsible for receiving, validating, and inventorying viruses that are utilized in BSL-4 research. This includes generating master and working stocks as well as maintaining an accurate inventory of agents. This core also develops advanced virologic tools as needed. The Viral Genomic Tracking and Single Cell omics Resource is responsible for ensuring the fidelity of viral stocks from VS given to investigators for their research, and for initiating single cell sequencing and single virus omics technologies in the BSL-4. The Imaging Services Unit provides several platforms to study living tissue specimens infected with BSL-4 pathogens, including Light Sheet microscopy. Many of the BSL-4 investigators rely on services from the NEIDL Comparative Pathology Laboratory (NCPL). Immunology Services provides training, guidance and maintenance of advanced immunology instrumentation inside the BSL-4 footprint, including flow cytometry. High-throughput screening has been operationalized to include automated visual screening of virus infected cells treated with small molecule inhibitors, and can be adapted to any virus or cell target. Linking the Clinic to NEIDL Programs includes a clinician whose responsibilities are to link clinical outcomes to the studies that are undertaken in the NEIDL (for comparative purposes) as well as to engage with clinical programs to obtain clinical samples for studies.