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Cortical mechanisms mediating visual function and behavior

介导视觉功能和行为的皮质机制

基本信息

批准号：
10665766
负责人：
Nicholas J Priebe
金额：
$ 52.67万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10665766
关键词：
Accounting Address Affect Anesthesia procedures Animals Attention Behavior Behavior Control Behavioral Biophysics Cells Cerebral cortex Complement Computer Models Data Set Detection Esthesia Event Facial Expression Future Goals Grant Laboratories Macaca Measurement Measures Mediating Membrane Membrane Potentials Modeling Monitor Monkeys Motivation Nature Neurons Noise Output Photic Stimulation Physiological Population Primates Property Pupil Recurrence Research Role Sensory Shapes Signal Transduction Source Stimulus Synapses Techniques Testing Time V1 neuron Variant Vision Visual Visual Perception Whole-Cell Recordings area striata awake behavioral response computational platform expectation experimental study individual response insight millimeter neural neural circuit neural model novel originality recurrent neural network response sample fixation sensory cortex visual control visual stimulus

项目摘要

Project Summary Intracellular recording from sensory cortex provides a window into the synaptic inputs that shape spiking responses of individual cortical neurons, but until recently, this powerful technique has been limited to anesthetized animals. By combining the unique expertise from our laboratories, we have developed a novel technique that allow us to conduct, on a routine basis, reliable, whole-cell intracellular recording in primary visual cortex (V1) of awake, behaving macaque monkeys. We combine intracellular recording with an array of concomitant measurements that provide access to the state of the local network in which the neuron is embedded as well as to the internal state of the animal. Using these techniques, we have access to both subthreshold (membrane potentials, representing input) and suprathreshold (spikes, representing output) responses of individual cortical neurons, while also utilizing the precise control of visual stimulation and the subject’s behavioral state afforded by behaving primates. Our ability to perform intracellular recording in awake, behaving primates opens the door to addressing three fundamental questions with respect to the circuit-level mechanisms that mediate visual perception: (1) what are the nature, sources, and behavioral consequences of the large neural variability of sensory cortical neurons, (2) what is the contribution of internal state fluctuations to this variability, (3) what circuit models can account for the observed neural variability during spontaneous and evoked responses? To address these questions, in Aim 1 we will study the quantitative relationship between sub- and suprathreshold activity during spontaneous and stimulus-evoked responses in V1 of fixating monkeys. This will allow us to test the generality of previous findings from anesthetized animals. In Aim 2, we will examine the relationship between the activity of single V1 neurons and perceptual decisions in monkeys that are engaged in a demanding visual detection task. Specifically, we will examine how sub- and suprathreshold responses are altered by changing the attentional and motivational states under which the stimulus is presented. Finally, in Aim 3 we will test a novel set of circuit models that can account for our observed results and guide future experiments.

项目摘要来自感觉皮层的细胞内记录提供了一个了解突触输入的窗口，单个皮层神经元的反应，但直到最近，这种强大的技术一直局限于麻醉动物。通过结合我们实验室的独特专业知识，我们开发了一种新颖的技术，使我们能够进行，在常规的基础上，可靠的，全细胞的细胞内记录在原代视觉皮层（V1）的清醒，行为猕猴。我们将联合收割机细胞内记录与一系列伴随的测量提供了对神经元在其中的局部网络的状态的访问，嵌入以及动物的内部状态。使用这些技术，我们可以访问阈下（膜电位，代表输入）和阈上（尖峰，代表输出）单个皮质神经元的反应，同时还利用视觉刺激的精确控制和受试者的行为状态由行为灵长类动物提供。我们在清醒的、行为正常的灵长类动物中进行细胞内记录的能力，为解决三个问题打开了大门。关于介导视觉感知的回路水平机制的基本问题：（1）什么是感觉皮层的大神经变异性的性质、来源和行为后果。神经元，（2）内部状态波动对这种可变性的贡献是什么，（3）什么电路模型能解释自发反应和诱发反应中观察到的神经变异吗为了解决这些问题，在目标1中，我们将研究子和子之间的定量关系。阈上活动在自发和刺激诱发的反应在V1的固定猴。这将使我们能够测试以前在麻醉动物身上发现的普遍性。在目标2中，我们将研究单个V1神经元的活动与猴子的感知决策之间的关系，从事要求严格的视觉检测任务。具体来说，我们将研究如何在阈下和阈上通过改变刺激所处的注意力和动机状态，提出了最后，在目标3中，我们将测试一组新的电路模型，这些模型可以解释我们观察到的结果并指导未来的实验。

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Voltage-Gated Intrinsic Conductances Shape the Input-Output Relationship of Cortical Neurons in Behaving Primate V1.

DOI：
10.1016/j.neuron.2020.04.001
发表时间：
2020-07-08
期刊：
NEURON
影响因子：
16.2
作者：
Li, Baowang;Routh, Brandy N.;Johnston, Daniel;Seidemann, Eyal;Priebe, Nicholas J.
通讯作者：
Priebe, Nicholas J.

Exact Analysis of the Subthreshold Variability for Conductance-Based Neuronal Models with Synchronous Synaptic Inputs