Core C - Genomics, Animal Models and Advanced Phenotyping Core

核心 C - 基因组学、动物模型和高级表型分析核心

• 批准号: 10403631
• 负责人: MICHAEL R GARRETT
• 金额: $ 38.62万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-05 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10403631
• 关键词: Animal Model; Animals; Biological Assay; Biological Process; Breeding; Cardiovascular Models; Cardiovascular system; Cells; Centers of Research Excellence; Clinical; Collection; Complement; Consultations; Country; Custom; Data Set; Disease; Ensure; Equipment; Faculty; Faculty Workshop; Fee-for-Service Plans; Funding; Gene Expression; Generations; Genes; Genetic Engineering; Genomics; Genotype; Goals; Grant; Health; High Prevalence; Historically Black Colleges and Universities; Inbred Mouse; Inbreeding; Infrastructure; Institution; Instruction; Kidney; Kidney Diseases; Knockout Mice; Letters; Maintenance; Measurement; Medical center; Metabolic Diseases; Methodology; Methylation; Mississippi; Modeling; Molecular; Molecular Genetics; Morbidity - disease rate; Obesity; Phase; Phenotype; Physiological; Population; Preparation; Proteins; Publications; Rattus; Research; Research Personnel; Research Project Grants; Scientific Advances and Accomplishments; Scientist; Services; Small RNA; Students; Supervision; Systems Biology; Technical Expertise; Techniques; Technology; Training; Training and Education; Transgenic Mice; Transgenic Organisms; United States; United States National Institutes of Health; Universities; Work; animal model development; base; bioinformatics tool; epigenetic regulation; epigenome; exome sequencing; genome sequencing; genomic data; improved; insight; instrumentation; meetings; metabolic phenotype; microbial; mortality; mouse model; multidisciplinary; next generation sequencing; novel; service delivery; training opportunity; transcriptome; transcriptome sequencing; undergraduate student; whole genome

CORE C – GENOMICS, ANIMAL MODELS AND ADVANCED PHENOTYPING PROJECT SUMMARY/ABSTRACT The goal of the Cardiorenal and Metabolic Disease Research Center (CMDRC) COBRE is to bring together a multidisciplinary group of basic, clinical, and population scientists to work synergistically on the theme of cardiorenal and metabolic diseases. During Phase 1, CMDRC-Core C provided COBRE investigators and external researchers' access to technical expertise and equipment to perform a wide variety of molecular, genetic, microarray and gene expression studies that would otherwise not be possible. Core C also provided investigators at UMMC access to novel transgenic and knockout mouse and rat models of cardiovascular renal and metabolic disease that are only available at UMMC. In total, CMDRC-Core C completed several hundred genomic studies as well as generated thousands of animals for detailed phenotyping resulting in numerous publications and several NIH R01 funded grants. In Phase II, CMDRC-Core C major project, pilot grants, and external researchers will have access to state-of-the-art genomic technologies through the Genomics Sub-Core and large collection of animal models (inbred, transgenic, and gene-edited rat and mouse models) as well as advanced phenotyping methodologies through the Animal Models and Advanced Phenotyping Sub-Core. Core C has four aims: (1) to provide CMDRC investigators access to technical expertise and cutting-edge genomic services, including next generation sequencing technologies (RNA sequencing, 16S microbial sequencing, custom amplicon panels, whole genome sequencing, and exome sequencing); (2) to provide CMDRC investigators access to large collection of unique animal models of obesity, cardiorenal and metabolic diseases, along with training in advanced phenotyping techniques; (3) to provide education and training opportunities for students, trainees, and faculty in genomics technologies and advanced animal phenotyping; and (4) to provide continuous improvement in existing assays, services, and enhance technological capabilities through acquisition of new cutting-edge instrumentation that will assist in sustainability of the Core. Therefore, it is the objective of the CMDRC-Core C to aid investigators in advancing scientific discovery in obesity, cardiorenal, and metabolic diseases through application of state-of-the-art genomic technologies, specialized animal models, and advanced physiological measurements to generate greater insight into these diseases. Aside from CMDRC investigators, continued funding of the COBRE during Phase II will have a significant impact on providing external researchers (at other Mississippi undergraduate institutions and Historically Black Colleges and Universities (HBCUs)] access to equipment and expertise that would otherwise not be available in our region of the country.

核心 C – 基因组学、动物模型和高级表型分析 项目概要/摘要 心肾和代谢疾病研究中心 (CMDRC) COBRE 的目标是将 由基础、临床和人口科学家组成的多学科小组，就以下主题协同工作 心肾和代谢疾病。在第一阶段，CMDRC-Core C 提供了 COBRE 研究人员和 外部研究人员获得技术专业知识和设备来执行各种分子、 原本不可能进行的遗传、微阵列和基因表达研究。还提供了核心C UMMC 的研究人员获得新型转基因和基因敲除小鼠和大鼠心血管模型 肾脏和代谢疾病仅在 UMMC 提供。总共，CMDRC-Core C 完成了多项 数百个基因组研究以及数千只动物进行详细的表型分析，从而产生 许多出版物和多项 NIH R01 资助的赠款。第二阶段，CMDRC-Core C重大项目，试点 赠款，外部研究人员将可以通过以下方式获得最先进的基因组技术 基因组学子核心和大量动物模型（近交系、转基因和基因编辑的大鼠和 小鼠模型）以及通过动物模型和高级表型分析方法 表型子核心。核心 C 有四个目标：(1) 为 CMDRC 调查人员提供技术支持 专业知识和尖端基因组服务，包括下一代测序技术（RNA 测序、16S 微生物测序、定制扩增子组合、全基因组测序和外显子组 测序）； (2) 为 CMDRC 研究人员提供大量独特的动物模型 肥胖、心肾和代谢疾病，以及先进表型分析技术的培训； (3) 至 为学生、实习生和教师提供基因组学技术方面的教育和培训机会 先进的动物表型分析； (4) 不断改进现有的检测、服务和 通过购买新的尖端仪器来增强技术能力，这将有助于 核心的可持续性。因此，CMDRC-Core C 的目标是帮助调查人员推进 通过应用最先进的技术对肥胖、心肾和代谢疾病进行科学发现 基因组技术、专门的动物模型和先进的生理测量来产生 对这些疾病有更深入的了解。除了 CMDRC 调查员之外，COBRE 在 第二阶段将对提供外部研究人员（密西西比州其他本科生）产生重大影响。 机构和传统黑人学院和大学 (HBCU)] 获得设备和专业知识 否则将无法在我们国家的地区使用。