Core C - Genomics, Animal Models and Advanced Phenotyping Core

核心 C - 基因组学、动物模型和高级表型分析核心

• 批准号: 10403631
• 负责人: MICHAEL R GARRETT
• 金额: $ 38.62万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-05 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10403631
• 关键词: Animal Model; Animals; Biological Assay; Biological Process; Breeding; Cardiovascular Models; Cardiovascular system; Cells; Centers of Research Excellence; Clinical; Collection; Complement; Consultations; Country; Custom; Data Set; Disease; Ensure; Equipment; Faculty; Faculty Workshop; Fee-for-Service Plans; Funding; Gene Expression; Generations; Genes; Genetic Engineering; Genomics; Genotype; Goals; Grant; Health; High Prevalence; Historically Black Colleges and Universities; Inbred Mouse; Inbreeding; Infrastructure; Institution; Instruction; Kidney; Kidney Diseases; Knockout Mice; Letters; Maintenance; Measurement; Medical center; Metabolic Diseases; Methodology; Methylation; Mississippi; Modeling; Molecular; Molecular Genetics; Morbidity - disease rate; Obesity; Phase; Phenotype; Physiological; Population; Preparation; Proteins; Publications; Rattus; Research; Research Personnel; Research Project Grants; Scientific Advances and Accomplishments; Scientist; Services; Small RNA; Students; Supervision; Systems Biology; Technical Expertise; Techniques; Technology; Training; Training and Education; Transgenic Mice; Transgenic Organisms; United States; United States National Institutes of Health; Universities; Work; animal model development; base; bioinformatics tool; epigenetic regulation; epigenome; exome sequencing; genome sequencing; genomic data; improved; insight; instrumentation; meetings; metabolic phenotype; microbial; mortality; mouse model; multidisciplinary; next generation sequencing; novel; service delivery; training opportunity; transcriptome; transcriptome sequencing; undergraduate student; whole genome

CORE C – GENOMICS, ANIMAL MODELS AND ADVANCED PHENOTYPING PROJECT SUMMARY/ABSTRACT The goal of the Cardiorenal and Metabolic Disease Research Center (CMDRC) COBRE is to bring together a multidisciplinary group of basic, clinical, and population scientists to work synergistically on the theme of cardiorenal and metabolic diseases. During Phase 1, CMDRC-Core C provided COBRE investigators and external researchers' access to technical expertise and equipment to perform a wide variety of molecular, genetic, microarray and gene expression studies that would otherwise not be possible. Core C also provided investigators at UMMC access to novel transgenic and knockout mouse and rat models of cardiovascular renal and metabolic disease that are only available at UMMC. In total, CMDRC-Core C completed several hundred genomic studies as well as generated thousands of animals for detailed phenotyping resulting in numerous publications and several NIH R01 funded grants. In Phase II, CMDRC-Core C major project, pilot grants, and external researchers will have access to state-of-the-art genomic technologies through the Genomics Sub-Core and large collection of animal models (inbred, transgenic, and gene-edited rat and mouse models) as well as advanced phenotyping methodologies through the Animal Models and Advanced Phenotyping Sub-Core. Core C has four aims: (1) to provide CMDRC investigators access to technical expertise and cutting-edge genomic services, including next generation sequencing technologies (RNA sequencing, 16S microbial sequencing, custom amplicon panels, whole genome sequencing, and exome sequencing); (2) to provide CMDRC investigators access to large collection of unique animal models of obesity, cardiorenal and metabolic diseases, along with training in advanced phenotyping techniques; (3) to provide education and training opportunities for students, trainees, and faculty in genomics technologies and advanced animal phenotyping; and (4) to provide continuous improvement in existing assays, services, and enhance technological capabilities through acquisition of new cutting-edge instrumentation that will assist in sustainability of the Core. Therefore, it is the objective of the CMDRC-Core C to aid investigators in advancing scientific discovery in obesity, cardiorenal, and metabolic diseases through application of state-of-the-art genomic technologies, specialized animal models, and advanced physiological measurements to generate greater insight into these diseases. Aside from CMDRC investigators, continued funding of the COBRE during Phase II will have a significant impact on providing external researchers (at other Mississippi undergraduate institutions and Historically Black Colleges and Universities (HBCUs)] access to equipment and expertise that would otherwise not be available in our region of the country.

核心C-基因组学、动物模型和高级表型 项目摘要/摘要 心肾和代谢性疾病研究中心(CMDRC)Cobre的目标是将 一个由基础、临床和人口科学家组成的多学科小组，共同致力于以下主题 心肾和代谢性疾病。在第一阶段，CMDRC-Core C为Cobre调查人员和 外部研究人员获得技术专业知识和设备以执行各种分子、 基因、微阵列和基因表达研究，否则是不可能的。核心C还提供了 UMMC的研究人员获得了新的心血管转基因和基因敲除小鼠和大鼠模型 只有在UMMC才有的肾脏和代谢性疾病。CMDRC-Core C总共完成了几项 数百项基因组研究以及产生了数千只动物进行详细的表型鉴定，结果是 许多出版物和几个NIH R01资助的赠款。在第二阶段，CMDRC-核心C主要项目，试点 赠款和外部研究人员将通过 基因组学亚核心和大型动物模型集合(近交系、转基因和基因编辑的大鼠和 小鼠模型)以及通过动物模型和高级 表型分型次核心。核心C有四个目标：(1)为CMDRC调查人员提供技术支持 专业知识和尖端基因组服务，包括下一代测序技术(RNA 测序、16S微生物测序、定制扩增子面板、全基因组测序和外显子组 测序)；(2)向CMDRC调查人员提供大量独特的动物模型集合 肥胖、心肾和代谢性疾病，以及高级表型技术培训； 为学生、实习生和教职员工提供基因组技术方面的教育和培训机会 先进的动物表型鉴定；以及(4)持续改进现有的检测、服务和 通过收购新的尖端仪器来增强技术能力，以帮助 核心的可持续性。因此，CMDRC-Core C的目标是帮助调查人员推进 通过应用最先进的技术在肥胖、心脏肾脏和代谢性疾病方面的科学发现 基因组技术、专门的动物模型和先进的生理测量方法 对这些疾病有更深入的了解。除了CMDRC调查人员外，Cobre在 第二阶段将对提供外部研究人员(在其他密西西比州本科生)产生重大影响 机构和传统黑人学院和大学(HBCU)]获得下列设备和专业知识 否则在我们国家的地区是不可用的。