Core C - Genomics, Animal Models and Advanced Phenotyping Core

核心 C - 基因组学、动物模型和高级表型分析核心

• 批准号: 10403631
• 负责人: MICHAEL R GARRETT
• 金额: $ 38.62万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-05 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10403631
• 关键词: Animal Model; Animals; Biological Assay; Biological Process; Breeding; Cardiovascular Models; Cardiovascular system; Cells; Centers of Research Excellence; Clinical; Collection; Complement; Consultations; Country; Custom; Data Set; Disease; Ensure; Equipment; Faculty; Faculty Workshop; Fee-for-Service Plans; Funding; Gene Expression; Generations; Genes; Genetic Engineering; Genomics; Genotype; Goals; Grant; Health; High Prevalence; Historically Black Colleges and Universities; Inbred Mouse; Inbreeding; Infrastructure; Institution; Instruction; Kidney; Kidney Diseases; Knockout Mice; Letters; Maintenance; Measurement; Medical center; Metabolic Diseases; Methodology; Methylation; Mississippi; Modeling; Molecular; Molecular Genetics; Morbidity - disease rate; Obesity; Phase; Phenotype; Physiological; Population; Preparation; Proteins; Publications; Rattus; Research; Research Personnel; Research Project Grants; Scientific Advances and Accomplishments; Scientist; Services; Small RNA; Students; Supervision; Systems Biology; Technical Expertise; Techniques; Technology; Training; Training and Education; Transgenic Mice; Transgenic Organisms; United States; United States National Institutes of Health; Universities; Work; animal model development; base; bioinformatics tool; epigenetic regulation; epigenome; exome sequencing; genome sequencing; genomic data; improved; insight; instrumentation; meetings; metabolic phenotype; microbial; mortality; mouse model; multidisciplinary; next generation sequencing; novel; service delivery; training opportunity; transcriptome; transcriptome sequencing; undergraduate student; whole genome

CORE C – GENOMICS, ANIMAL MODELS AND ADVANCED PHENOTYPING PROJECT SUMMARY/ABSTRACT The goal of the Cardiorenal and Metabolic Disease Research Center (CMDRC) COBRE is to bring together a multidisciplinary group of basic, clinical, and population scientists to work synergistically on the theme of cardiorenal and metabolic diseases. During Phase 1, CMDRC-Core C provided COBRE investigators and external researchers' access to technical expertise and equipment to perform a wide variety of molecular, genetic, microarray and gene expression studies that would otherwise not be possible. Core C also provided investigators at UMMC access to novel transgenic and knockout mouse and rat models of cardiovascular renal and metabolic disease that are only available at UMMC. In total, CMDRC-Core C completed several hundred genomic studies as well as generated thousands of animals for detailed phenotyping resulting in numerous publications and several NIH R01 funded grants. In Phase II, CMDRC-Core C major project, pilot grants, and external researchers will have access to state-of-the-art genomic technologies through the Genomics Sub-Core and large collection of animal models (inbred, transgenic, and gene-edited rat and mouse models) as well as advanced phenotyping methodologies through the Animal Models and Advanced Phenotyping Sub-Core. Core C has four aims: (1) to provide CMDRC investigators access to technical expertise and cutting-edge genomic services, including next generation sequencing technologies (RNA sequencing, 16S microbial sequencing, custom amplicon panels, whole genome sequencing, and exome sequencing); (2) to provide CMDRC investigators access to large collection of unique animal models of obesity, cardiorenal and metabolic diseases, along with training in advanced phenotyping techniques; (3) to provide education and training opportunities for students, trainees, and faculty in genomics technologies and advanced animal phenotyping; and (4) to provide continuous improvement in existing assays, services, and enhance technological capabilities through acquisition of new cutting-edge instrumentation that will assist in sustainability of the Core. Therefore, it is the objective of the CMDRC-Core C to aid investigators in advancing scientific discovery in obesity, cardiorenal, and metabolic diseases through application of state-of-the-art genomic technologies, specialized animal models, and advanced physiological measurements to generate greater insight into these diseases. Aside from CMDRC investigators, continued funding of the COBRE during Phase II will have a significant impact on providing external researchers (at other Mississippi undergraduate institutions and Historically Black Colleges and Universities (HBCUs)] access to equipment and expertise that would otherwise not be available in our region of the country.

核心C -基因组学、动物模型和高级表型分析 项目总结/摘要 肾脏和代谢疾病研究中心（CMDRC）COBRE的目标是汇集 一个由基础、临床和人口科学家组成的多学科小组，协同工作， 心肾和代谢疾病。在第1阶段，CMDRC核心C提供了COBRE研究者， 外部研究人员可以获得技术专长和设备， 基因，微阵列和基因表达研究，否则将是不可能的。核心C还提供了 UMMC的研究人员获得了新的转基因和基因敲除小鼠和大鼠心血管模型 肾脏和代谢疾病，只有在UMMC可用。总的来说，CMDRC核心C完成了几个 数百项基因组研究以及产生数千只动物进行详细的表型分析， 许多出版物和几个NIH R 01资助的赠款。在第二阶段，CMDRC-核心C重大项目，试点 赠款，外部研究人员将有机会获得最先进的基因组技术，通过 基因组学子核心和大量动物模型（近交系、转基因和基因编辑的大鼠和 小鼠模型）以及通过动物模型和高级 表型亚核心。核心C有四个目标：（1）为CMDRC调查人员提供技术 专业知识和尖端的基因组服务，包括下一代测序技术（RNA 测序、16 S微生物测序、定制扩增子面板、全基因组测序和外显子组 测序）;（2）为CMDRC研究人员提供大量独特的动物模型， 肥胖、心肾和代谢疾病，沿着高级表型技术的培训;（3） 为学生、学员和教职员工提供基因组学技术方面的教育和培训机会， 先进的动物表型分析;以及（4）在现有的检测、服务和 通过采购新的尖端仪器来提高技术能力， 核心的可持续性。因此，CMDRC核心C的目的是帮助研究者推进 通过应用最先进的技术，在肥胖、心肾和代谢疾病方面的科学发现 基因组技术、专门的动物模型和先进的生理测量， 对这些疾病有更深入的了解。除了CMDRC的调查人员外，COBRE在2009年期间继续获得资金， 第二阶段将对提供外部研究人员产生重大影响（在其他密西西比本科生中 机构和历史上的黑人学院和大学（HBCU）]获得设备和专业知识， 否则在我国的这一地区将无法获得。