Core B: Biorepository and Biospecimen Resource Facility Core

核心 B：生物样本库和生物样本资源设施核心

• 批准号: 10630467
• 负责人: Brock Clarke Christensen
• 金额: $ 39.42万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630467
• 关键词: Administrative Supplement; Aliquot; Archives; Biocompatible Materials; Bioinformatics; Biological; Biological Assay; Businesses; Cells; Centers of Research Excellence; Chemicals; Clinical Laboratory Information Systems; Code; Collaborations; Collection; Communities; Complex; Consultations; Core Facility; DNA; Data; Disease; Educational Materials; Equipment; Fees; Funding; Future; Genomics; Human; Information Management; Information Systems; Infrastructure; Institution; International; Investments; Laboratories; Link; Marketing; Masks; Measures; Metagenomics; Mission; Modernization; Molecular; Molecular Epidemiology; Monitor; Participant; Performance; Phase; Pilot Projects; Procedures; Process; Protocols documentation; Quality Control; Randomized; Research; Research Design; Research Personnel; Resources; Robotics; Sampling; Schools; Secure; Security; Services; Site; Specimen; Strategic Planning; System; Technology; Training; Translational Research; Triage; United States National Institutes of Health; Update; base; biobank; commercialization; design; epidemiology study; experience; flexibility; genome-wide analysis; human disease; improved; investigator training; member; metabolomics; multidisciplinary; novel; programs; protocol development

ABSTRACT The basis of molecular epidemiologic research lies in the combination of biospecimens and linked subject data. Over the past two phases of our COBRE Center for Molecular Epidemiology, we have created a unique Biorepository and Biorespecimen Resource Facility Core with a state-of-the-art and internationally competitive infrastructure incorporating advanced robotic and bioinformatic systems. Careful handling of biologic samples with well-integrated de-identified specimen coding, tracking, and data systems are essential for applying cutting-edge technologies to rapidly advance our understanding of the molecular basis of complex disease interactions. The Core has handled over 400,000 diverse samples, created over 270,000 aliquots, and assembled and distributed over 50,000 specimen sets to specialized laboratories. Reflecting the high value of the Core to the insitutional research mission, the Biospecimen Resource Facility – containing over 615,000 samples – expanded into a larger, on-site, and newly renovated space with modernized security and back-up systems for long-term biospecimen storage. An advanced laboratory information management and biospecimen tracking system has been implemented, and our automated equipment has been upgraded for even higher throughput sample processing with flexible configuration to assimilate future technological advancements. The wealth of experience, optimized protocols, and efficient systems management approaches in the Biorepository Core allow the support of a broad range of downstream analyses including novel chemical assays, genome wide analyses, metabolomics, and metagenomics. The Core has continued to expand its ability to accommodate diverse specimen types and applications including processing, archiving, and isolations for single cell genomics applications. In Phase III, we will: expand our biorepository services (Aim 1); grow our user base by creating and disseminating materials about our offerings (Aim 2); and extend and adapt our array of services for the wider research community, while continuously evaluating our performance and repertoire of services and developing a strategic plan to achieve long-term sustainability (Aim 3). By providing a centralized and integrated biorepository resource, including study design consultation, we will continue to i) enable efficient, appropriate, and safe collection and storage of biomaterials, ii) utilize quality-controlled sample processing, archiving, and extraction procedures, iii) provide access to experienced investigators with decades of experience in human sample storage and use, and iv) facilitate collaboration and integration across projects and investigators. In Phase III, we will refine our marketing and financial plans for sustainability, and train investigators on ways to expedite research translation and commercialization. Thus, we will continue this vital and successful Core to support new initiatives seeded by the Pilot Project Program, along with regional and national efforts to reduce the burden of human disease.

抽象的 分子流行病学研究的基础在于生物样本和相关主题数据的结合。 在 COBRE 分子流行病学中心的过去两个阶段中，我们创建了一个独特的 拥有最先进且具有国际竞争力的生物样本库和生物样本资源设施核心 融合先进机器人和生物信息系统的基础设施。小心处理生物样本 具有良好集成的去识别样本编码、跟踪和数据系统对于应用至关重要 尖端技术可快速增进我们对复杂疾病分子基础的理解 互动。该核心已处理了超过 400,000 个不同的样本，创建了超过 270,000 个等分试样，并且 组装并分发了超过 50,000 个样本组到专业实验室。体现出高价值 机构研究任务的核心——生物样本资源设施——包含超过 615,000 个样本 样品 - 扩展到更大的现场新装修空间，配备现代化的安全和备份 用于长期生物样本储存的系统。先进的实验室信息管理和 生物样本追踪系统已实施，自动化设备已升级 通过灵活的配置实现更高通量的样品处理，以吸收未来的技术 进步。丰富的经验、优化的协议和高效的系统管理方法 生物样本库核心允许支持广泛的下游分析，包括新型化学分析 分析、全基因组分析、代谢组学和宏基因组学。核心不断扩大 能够适应不同的样本类型和应用，包括处理、存档和隔离 用于单细胞基因组学应用。在第三阶段，我们将： 扩大我们的生物样本库服务（目标 1）；发展我们的 通过创建和传播有关我们产品的材料来扩大用户群（目标 2）；并扩展和调整我们的数组 为更广泛的研究界提供服务，同时不断评估我们的表现和技能 服务并制定战略计划以实现长期可持续性（目标 3）。通过提供集中式 和综合生物样本库资源，包括研究设计咨询，我们将继续 i) 启用 有效、适当和安全地收集和储存生物材料，ii) 使用质量受控的样品 处理、存档和提取程序，iii) 为数十年经验丰富的研究人员提供机会 人类样本储存和使用方面的经验，以及 iv) 促进跨项目的协作和集成 和调查人员。在第三阶段，我们将完善可持续发展的营销和财务计划，并培训 研究人员如何加快研究转化和商业化。因此，我们将继续这一重要的 和成功的核心支持试点项目计划以及区域和 国家努力减轻人类疾病负担。