Core B: Biorepository and Biospecimen Resource Facility Core

核心 B：生物样本库和生物样本资源设施核心

• 批准号: 10630467
• 负责人: Brock Clarke Christensen
• 金额: $ 39.42万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630467
• 关键词: Administrative Supplement; Aliquot; Archives; Biocompatible Materials; Bioinformatics; Biological; Biological Assay; Businesses; Cells; Centers of Research Excellence; Chemicals; Clinical Laboratory Information Systems; Code; Collaborations; Collection; Communities; Complex; Consultations; Core Facility; DNA; Data; Disease; Educational Materials; Equipment; Fees; Funding; Future; Genomics; Human; Information Management; Information Systems; Infrastructure; Institution; International; Investments; Laboratories; Link; Marketing; Masks; Measures; Metagenomics; Mission; Modernization; Molecular; Molecular Epidemiology; Monitor; Participant; Performance; Phase; Pilot Projects; Procedures; Process; Protocols documentation; Quality Control; Randomized; Research; Research Design; Research Personnel; Resources; Robotics; Sampling; Schools; Secure; Security; Services; Site; Specimen; Strategic Planning; System; Technology; Training; Translational Research; Triage; United States National Institutes of Health; Update; base; biobank; commercialization; design; epidemiology study; experience; flexibility; genome-wide analysis; human disease; improved; investigator training; member; metabolomics; multidisciplinary; novel; programs; protocol development

ABSTRACT The basis of molecular epidemiologic research lies in the combination of biospecimens and linked subject data. Over the past two phases of our COBRE Center for Molecular Epidemiology, we have created a unique Biorepository and Biorespecimen Resource Facility Core with a state-of-the-art and internationally competitive infrastructure incorporating advanced robotic and bioinformatic systems. Careful handling of biologic samples with well-integrated de-identified specimen coding, tracking, and data systems are essential for applying cutting-edge technologies to rapidly advance our understanding of the molecular basis of complex disease interactions. The Core has handled over 400,000 diverse samples, created over 270,000 aliquots, and assembled and distributed over 50,000 specimen sets to specialized laboratories. Reflecting the high value of the Core to the insitutional research mission, the Biospecimen Resource Facility – containing over 615,000 samples – expanded into a larger, on-site, and newly renovated space with modernized security and back-up systems for long-term biospecimen storage. An advanced laboratory information management and biospecimen tracking system has been implemented, and our automated equipment has been upgraded for even higher throughput sample processing with flexible configuration to assimilate future technological advancements. The wealth of experience, optimized protocols, and efficient systems management approaches in the Biorepository Core allow the support of a broad range of downstream analyses including novel chemical assays, genome wide analyses, metabolomics, and metagenomics. The Core has continued to expand its ability to accommodate diverse specimen types and applications including processing, archiving, and isolations for single cell genomics applications. In Phase III, we will: expand our biorepository services (Aim 1); grow our user base by creating and disseminating materials about our offerings (Aim 2); and extend and adapt our array of services for the wider research community, while continuously evaluating our performance and repertoire of services and developing a strategic plan to achieve long-term sustainability (Aim 3). By providing a centralized and integrated biorepository resource, including study design consultation, we will continue to i) enable efficient, appropriate, and safe collection and storage of biomaterials, ii) utilize quality-controlled sample processing, archiving, and extraction procedures, iii) provide access to experienced investigators with decades of experience in human sample storage and use, and iv) facilitate collaboration and integration across projects and investigators. In Phase III, we will refine our marketing and financial plans for sustainability, and train investigators on ways to expedite research translation and commercialization. Thus, we will continue this vital and successful Core to support new initiatives seeded by the Pilot Project Program, along with regional and national efforts to reduce the burden of human disease.

摘要 分子流行病学研究的基础在于生物标本和相关主题数据的结合。 在我们科布雷分子流行病学中心的过去两个阶段中，我们创建了一个独特的 具有最先进和国际竞争力的生物信息库和生物尊重资源设施核心 结合先进的机器人和生物信息系统的基础设施。仔细处理生物样本 有了集成良好的去识别样本编码、跟踪和数据系统，应用 尖端技术迅速提高我们对复杂疾病的分子基础的理解 互动。核心已经处理了超过400,000个不同的样品，创建了超过270,000个等分，以及 组装了50,000多套标本，并将其分发给专业实验室。反映了的高度价值 机构研究任务的核心--生物谱系资源设施--包含615,000多个 样品-扩展为一个更大的、现场的、新装修的空间，配备现代化的安全和备份 用于长期储存生物样品的系统。先进的实验室信息管理和 已经实施了Biosecimen跟踪系统，我们的自动化设备已经升级 通过灵活的配置实现更高的样品处理吞吐量，以吸收未来的技术 进步。丰富的经验、优化的协议和高效的系统管理方法 在生物库核心中允许支持广泛的下游分析，包括新的化学物质 分析、全基因组分析、代谢组学和元基因组学。核心继续扩大其 能够适应不同的标本类型和应用，包括处理、归档和分离 用于单细胞基因组学应用。在第三阶段，我们将：扩大我们的生物库服务(目标1)；发展我们的 通过创建和传播有关我们产品的材料(目标2)建立用户基础；并扩展和调整我们的阵列 为更广泛的研究社区提供服务，同时不断评估我们的表现和曲目 并制定实现长期可持续性的战略计划(目标3)。通过提供集中的 和整合生物库资源，包括研究设计咨询，我们将继续i)启用 有效、适当和安全地收集和储存生物材料，ii)使用质量受控样本 处理、存档和提取程序，三)为有经验的调查人员提供几十年的机会 人类样本储存和使用方面的经验；以及iv)促进项目间的协作和整合 和调查人员。在第三阶段，我们将完善我们的营销和财务可持续发展计划，并培训 研究人员就如何加快研究、翻译和商业化进行了研究。因此，我们将继续这一至关重要的 和成功的核心，以支持由试点项目方案播种的新倡议，以及区域和 各国为减轻人类疾病负担所作的努力。