2-photon imaging system

2光子成像系统

• 批准号: 7792526
• 负责人: Anthony J Ricci
• 金额: $ 38.77万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-03 至 2011-06-02
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792526
• 关键词: Acoustics; Amplifiers; Animals; Auditory; Basilar Membrane; Brain region; Cells; Cochlea; Contract Services; Core Facility; Devices; Equipment; Funding; Hearing; Human Resources; Image; Laboratories; Life; Maintenance; Measurement; Mechanics; Nose; Photons; Refractive Indices; Research; Research Personnel; Research Project Grants; Resources; Sensory Hair; Structure; System; Technology; Tissues; Training; Tympanometry; abstracting; cost; graduate student; instrument; lens; micromanipulator; molecular imaging; new technology; otoacoustic emission; research study; tectorial membrane; two-photon

DESCRIPTION (provided by applicant): We are proposing to purchase a two photon mating system that is specialized for microendoscopy. The gradient refractive index (GRIN) lens microendoscope allows for imaging of previously inaccessible tissue. For our research core, this means imaging the cochlea, though this is not exclusive. Along with this system, we need to incorporate the ability to assess quality of hearing, which includes, ABR, distortion product otoacoustic emissions and tympanometry. Additionally, the ability to make electrophysiological measurements is required and so micromanipulators and an amplifier are requested. This device will expand our imaging core so that whole animal hearing experiments can be performed at the cellular and systems level. This technology will give us unprecedented access to living cochlea tissue, greatly expanding and enhancing ongoing research projects within this core. To date, most measurements of cochlea mechanics are single or dual point measurements at the level of the basilar membrane. This new technology allows imaging of multiple cells or cell parts, like sensory hair bundles, tectorial membrane, etc. Proof of principal experiments have been performed with a consultant and expert on GRIN lens (Mark Schnitzer) but are limited by being in a laboratory not equipped for acoustic isolation and on a system that is not dedicated to these experiments. This device has the potential to revolutionize auditory research in the whole animal and will expand the resources of our imaging core to incorporate imaging from the molecular to cellular to system. The technology is not limited to cochlear exploration and as demonstrated in the research projects section can be used to investigate heretofore inaccessible structures in living animals, such as deep brain regions and nasal mucosal cells. Together these projects incorporate 6 presently funded RO1s from 4 investigators representing five different departments. Also there are presently three junior investigators actively pursuing this technology. Part of the mandate of our core facility is to provide access and training to graduate students and residents and so the addition of this technology will greatly enhance training. In addition, this technology allows direct translational experiments to be performed in the whole animal. We have significant departmental support funding the service contract for the instrument, support personnel involved in the training and maintenance of the equipment and the setup costs for the equipment. PUBLIC HEALTH RELEVANCE: Hearing loss induced by noise exposure, aging or chemicals reduce quality of life and productivity of millions of people each year, costing our economy billions of dollars. The ability to directly assess the actions of chemicals or noise on the sensory tissue has long been hampered by the inability to access cochlear tissue, as it is surrounded by bone. The equipment requested, a two photon imaging system using microendoscopy allows minimally invasive surgery to result in our ability to directly monitor cochlea tissue in vivo and thus will expedite our understanding of the mechanisms associated with these forms of hearing loss.

描述（由申请人提供）：我们建议购买一种专门用于显微内窥镜检查的双光子配对系统。梯度折射率（GRIN）透镜显微内窥镜允许对先前不可接近的组织进行成像。对于我们的研究核心，这意味着对耳蜗进行成像，尽管这不是唯一的。沿着该系统，我们需要结合评估听力质量的能力，包括ABR、畸变产物耳声发射和鼓室压测定。此外，需要进行电生理测量的能力，因此需要显微操作器和放大器。该设备将扩展我们的成像核心，使整个动物听力实验可以在细胞和系统水平上进行。这项技术将使我们前所未有地获得活的耳蜗组织，大大扩展和加强这一核心内正在进行的研究项目。迄今为止，大多数耳蜗力学的测量是在基底膜水平上的单点或双点测量。这种新技术允许成像的多个细胞或细胞部分，如感觉毛束，覆膜等证明的主要实验已经进行了与顾问和专家的GRIN透镜（马克Schnitzer），但被限制在一个实验室不配备声学隔离和系统，不专门用于这些实验。该设备有可能彻底改变整个动物的听觉研究，并将扩大我们的成像核心资源，将成像从分子到细胞再到系统。该技术并不局限于耳蜗探测，如研究项目部分所示，可用于研究活体动物中迄今无法触及的结构，如脑深部区域和鼻粘膜细胞。这些项目包括来自代表五个不同部门的四名调查员的六个目前得到资助的一级区域办事处。此外，目前有三名初级调查员积极研究这项技术。我们核心设施的部分任务是为研究生和住院医生提供访问和培训，因此这项技术的增加将大大加强培训。此外，该技术允许在整个动物中进行直接翻译实验。我们有大量的部门支持，为仪器的服务合同、参与设备培训和维护的支持人员以及设备的安装成本提供资金。 公共卫生关系：噪声暴露、老化或化学品导致的听力损失每年降低数百万人的生活质量和生产力，使我们的经济损失数十亿美元。直接评估化学物质或噪音对感觉组织的作用的能力长期以来一直受到无法进入耳蜗组织的阻碍，因为它被骨骼包围。所要求的设备，一个双光子成像系统，使用显微内窥镜允许微创手术，使我们能够直接监测体内耳蜗组织，从而加快我们的理解与这些形式的听力损失的机制。