Mechanistic Insights into the Plant Disease Resistance Mediated by NPR1

NPR1 介导的植物抗病性的机制见解

• 批准号: 10670797
• 负责人: Pei Zhou
• 金额: $ 36.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10670797
• 关键词: Achievement; Agriculture; Ankyrin Repeat; Arabidopsis; Architecture; Aspirin; BTB/POZ Domain; Binding; Biochemical; Biological Process; Biological Response Modifiers; Biology; Birds; C-terminal; CUL3 gene; Cardiovascular Diseases; Cell Nucleus; Cell Survival; Cells; Cessation of life; Circadian Rhythms; Complex; Cryoelectron Microscopy; Cullin Proteins; Cytoplasm; DNA Binding Domain; Dimerization; Disease; Disease Resistance; Dissociation; Endoplasmic Reticulum; Ensure; Event; Fever; Gene Activation; Gene Expression; Genes; Genetic; Genetic Engineering; Genetic Transcription; Goals; Health Benefit; Human; Immune response; Immunity; Investigation; Knowledge; Length; Malignant Neoplasms; Masks; Mediating; Medicine; Molecular; Molecular Conformation; Mouse-ear Cress; N-terminal; Natural Immunity; Nature; Nuclear; Nuclear Translocation; Oxidation-Reduction; Pathogenesis; Pathogenicity; Pesticides; Phase; Plant Diseases; Plants; Polyubiquitination; Process; Proteins; Recombinants; Regulation; Resistance; Resolution; Rest; Role; Salicylic Acids; Signal Pathway; Signal Transduction; Stress; Structure; Testing; Transcription Coactivator; Transcription Repressor; Visualization; Wing; analog; circadian regulation; cofactor; cullin-3; dimer; disulfide bond; fitness; genome-wide; human disease; insight; mutant; pain relief; particle; pathogen; pesticide resistance; pressure; prevent; proteotoxicity; public health relevance; receptor; recruit; response; success; transcription factor; transcriptional reprogramming; ubiquitin-protein ligase

Project Summary/Abstract NPR1 (NONEXPRESSOR OF PATHOGENSIS-RELATED GENES 1) is a master immune regulator in plants. It orchestrates systemic acquired resistance (SAR) by activating PATHOGENESIS-RELATED (PR) genes in response to induction of salicylic acid (SA) during the plant response to pathogenic challenges. Extensive genetic and biochemical studies have shown that NPR1 is a receptor of SA. SA activation of NPR1 triggers genome- wide transcriptional reprograming via NPR1 interactions with a variety of transcription activators and repressors. NPR1 itself is regulated by redox agents, and its proteolytic turnover has profound implications in a wide range of biological functions, including circadian rhythm and resistance to proteotoxic stress in the endoplasmic reticulum. Despite the essential role of NPR1 in plant biology, the molecular details underlying the myriad of NPR1 functions have remained largely unknown. Building upon our initial success in elucidating the structure of apo NPR1 using single-particle cryoEM, here we propose to elucidate the molecular basis of the NPR1 function in plants. Specifically, we will address (1) how SA activates NPR1, (2) how NPR1 interacts with transcription factors and regulators, and (3) how NPR1 is degraded. Information gained from these studies will provide the much-needed mechanistic insights into the SA-NPR1 signaling cascade in plants and help develop disease- resistant crops. As SA is the principle metabolite of aspirin, such knowledge may also contribute to a better understanding of the well-documented medicinal benefits of SA and analogs in humans.

项目概要/摘要 NPR1（致病相关基因非表达基因 1）是植物中的主要免疫调节剂。它 通过激活致病相关（PR）基因来协调系统获得性耐药（SAR） 在植物对病原挑战的反应过程中对水杨酸（SA）诱导的反应。广泛的遗传 生化研究表明NPR1是SA的受体。 NPR1 的 SA 激活触发基因组- 通过 NPR1 与多种转录激活子和阻遏子的相互作用进行广泛的转录重编程。 NPR1本身受到氧化还原剂的调节，其蛋白水解转换在广泛的领域具有深远的影响 生物功能，包括昼夜节律和内质蛋白毒性应激的抵抗力 网状结构。尽管 NPR1 在植物生物学中发挥着重要作用，但其背后的分子细节却是无数的。 NPR1 的功能在很大程度上仍然未知。在我们阐明结构的初步成功的基础上 使用单粒子冷冻电镜分析 apo NPR1，在这里我们建议阐明 NPR1 功能的分子基础 在植物中。具体来说，我们将讨论 (1) SA 如何激活 NPR1，(2) NPR1 如何与转录相互作用 因素和监管机构，以及 (3) NPR1 如何降解。从这些研究中获得的信息将提供 对植物中 SA-NPR1 信号级联的急需机制见解并有助于疾病的发展- 抗性作物。由于 SA 是阿司匹林的主要代谢物，因此这些知识也可能有助于更好地了解 了解 SA 及其类似物对人类的有据可查的医学益处。