Mechanistic Insights into the Plant Disease Resistance Mediated by NPR1

NPR1 介导的植物抗病性的机制见解

• 批准号: 10390811
• 负责人: Pei Zhou
• 金额: $ 36.62万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10390811
• 关键词: Achievement; Address; Agriculture; Ankyrin Repeat; Arabidopsis; Architecture; Aspirin; BTB/POZ Domain; Binding; Biochemical; Biological Process; Biological Response Modifiers; Biology; C-terminal; CUL3 gene; Cardiovascular Diseases; Cell Nucleus; Cell Survival; Cells; Cessation of life; Circadian Rhythms; Complex; Cytoplasm; DNA Binding Domain; Dimerization; Disease; Disease Resistance; Dissociation; Electron Microscopy; Endoplasmic Reticulum; Ensure; Event; Fever; Gene Activation; Gene Expression; Genes; Genetic; Genetic Engineering; Genetic Transcription; Goals; Health Benefit; Human; Immune response; Immunity; Investigation; Knowledge; Length; Malignant Neoplasms; Masks; Mediating; Molecular; Molecular Conformation; Mouse-ear Cress; N-terminal; Natural Immunity; Nature; Nuclear; Nuclear Translocation; Oxidation-Reduction; Pathogenesis; Pathogenicity; Pesticides; Plant Diseases; Plants; Polyubiquitination; Process; Proteins; Recombinants; Regulation; Resistance; Resolution; Rest; Role; Salicylic Acids; Signal Pathway; Signal Transduction; Stress; Structure; Testing; Transcription Coactivator; Transcription Repressor; Ursidae Family; Wing; analog; base; circadian regulation; cofactor; cryogenics; cullin-3; dimer; disulfide bond; fitness; genome-wide; human disease; insight; mutant; pain relief; particle; pathogen; pesticide resistance; pressure; prevent; proteotoxicity; public health relevance; receptor; recruit; response; success; transcription factor; transcriptional reprogramming; ubiquitin-protein ligase

Project Summary/Abstract NPR1 (NONEXPRESSOR OF PATHOGENSIS-RELATED GENES 1) is a master immune regulator in plants. It orchestrates systemic acquired resistance (SAR) by activating PATHOGENESIS-RELATED (PR) genes in response to induction of salicylic acid (SA) during the plant response to pathogenic challenges. Extensive genetic and biochemical studies have shown that NPR1 is a receptor of SA. SA activation of NPR1 triggers genome- wide transcriptional reprograming via NPR1 interactions with a variety of transcription activators and repressors. NPR1 itself is regulated by redox agents, and its proteolytic turnover has profound implications in a wide range of biological functions, including circadian rhythm and resistance to proteotoxic stress in the endoplasmic reticulum. Despite the essential role of NPR1 in plant biology, the molecular details underlying the myriad of NPR1 functions have remained largely unknown. Building upon our initial success in elucidating the structure of apo NPR1 using single-particle cryoEM, here we propose to elucidate the molecular basis of the NPR1 function in plants. Specifically, we will address (1) how SA activates NPR1, (2) how NPR1 interacts with transcription factors and regulators, and (3) how NPR1 is degraded. Information gained from these studies will provide the much-needed mechanistic insights into the SA-NPR1 signaling cascade in plants and help develop disease- resistant crops. As SA is the principle metabolite of aspirin, such knowledge may also contribute to a better understanding of the well-documented medicinal benefits of SA and analogs in humans.

项目总结/摘要 NPR 1（Nonexpressorofpathogenis-relatedgenes 1）是植物的主要免疫调节因子。它 通过激活病原学相关（PR）基因协调全身获得性耐药（SAR）， 在植物对病原体挑战的响应期间，对水杨酸（SA）诱导的响应。广泛的遗传 生化研究表明NPR 1是SA的受体。SA激活NPR 1触发基因组- 广泛的转录重编程通过NPR 1与各种转录激活因子和抑制因子的相互作用。 NPR 1本身受氧化还原剂的调节，其蛋白水解周转在广泛的范围内具有深远的意义 生物学功能，包括昼夜节律和抵抗内质网中的蛋白毒性应激 网状细胞。尽管NPR 1在植物生物学中起着重要作用，但其背后的无数分子细节仍然存在。 NPR 1的功能在很大程度上仍然未知。在我们初步成功地阐明了 apo NPR 1使用单粒子cryoEM，在这里，我们建议阐明NPR 1功能的分子基础 在植物中。具体来说，我们将解决（1）SA如何激活NPR 1，（2）NPR 1如何与转录相互作用 因子和调节因子;（3）NPR 1是如何降解的。从这些研究中获得的信息将提供 对植物中SA-NPR 1信号级联的迫切需要的机制见解，并帮助发展疾病- 抗性作物由于SA是阿司匹林的主要代谢产物，因此这些知识也有助于更好地 了解SA和类似物在人类中的良好记录的医学益处。