PRINCIPLES OF PRESYNAPTIC NETWORKS FOR SINGLE LAYER 2/3 NEURONS IN FERRET VISUAL CORTEX

雪貂视皮层单层2/3神经元的突触前网络原理 新皮质环路中的单个神经元由兴奋性和抑制性组成的突触前网络驱动 神经元。每个神经元的突触前伙伴群体决定了传入信息的处理方式。 长期以来，对大脑皮层回路的一种观点认为，大多数突触输入来自局部网络，通过 水平(循环)连接。然而，递归网络塑造活动的机制 大脑皮层神经元在很大程度上是未知的。此外，理论上提出的突触和细胞机制 模型依赖于对啮齿动物视觉皮质的研究，越来越多的研究表明，啮齿动物的视觉皮质与食肉动物的视觉皮质不同 和灵长类动物在组织和功能上。这项提案旨在通过绘制地图来解决这些问题 单层2/3神经元突触前兴奋性和抑制性细胞及其选择性作用的解剖 调节体内雪貂V1的神经活动。该方案使用了先进的光学技术的新组合 技术和电生理学，建立在候选人的基础上 光学式 创建 在体内生理学方面有深厚的背景 在各种哺乳动物中进行成像，并在K99阶段进行训练。这项提议将 为建立创新、多学科和独立的研究计划奠定基础 大脑皮层回路的基本原理，最终为理解障碍提供了一个框架，例如 精神分裂症和自闭症，表现出严重的感觉信号处理障碍。

Principles of presynaptic networks for single layer 2/3 neurons in ferret visual cortex Single neurons in neocortical circuits are driven by presynaptic networks composed of excitatory and inhibitory neurons. Each neuron's population of presynaptic partners determines how incoming information is processed. A longstanding view of cortical circuits is that a majority of synaptic inputs originate from local networks through horizontal (recurrent) connections. However, the mechanisms by which recurrent networks shape the activity of cortical neurons is largely unknown. Additionally, synaptic and cellular mechanisms proposed by theoretical models rely on studies of the rodent visual cortex, which is increasingly shown to differ from that of carnivores and primates in organization and function. This proposal aims to address these problems by mapping presynaptic excitatory and inhibitory cells of single layer 2/3 neurons and dissect how they act to selectively modulate neural activity in ferret V1 in vivo. This proposal uses a novel combination of advanced optical techniques and electrophysiology, building off the candidates optical create has a deep background in in vivo physiology and imaging in a wide variety of mammalian species and training during the K99 phase. This proposal will the foundation for an innovative, multidisciplinary, and independent research program to establish fundamental principles of cortical circuits, ultimately providing a scaffold for understanding disorders, such as schizophrenia and autism, which show profound impairments in the processing of sensory signals.