PRINCIPLES OF PRESYNAPTIC NETWORKS FOR SINGLE LAYER 2/3 NEURONS IN FERRET VISUAL CORTEX

雪貂视觉皮层单层 2/3 神经元突触前网络原理

• 批准号: 10675228
• 负责人: Benjamin Kyle Scholl
• 金额: $ 12.86万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10675228
• 关键词: Address; Cells; Disease; Electrophysiology (science); Ferrets; Foundations; Goals; Image; Impairment; Neurons; Optics; Phase; Physiology; Population; Primates; Process; Recurrence; Research; Rodent; Role; Schizophrenia; Sensory; Shapes; Signal Transduction; Synapses; Techniques; Theoretical model; Training; Visual Cortex; autism spectrum disorder; excitatory neuron; in vivo; inhibitory neuron; innovation; insight; multidisciplinary; neocortical; novel; presynaptic; programs; relating to nervous system; scaffold; signal processing

Principles of presynaptic networks for single layer 2/3 neurons in ferret visual cortex Single neurons in neocortical circuits are driven by presynaptic networks composed of excitatory and inhibitory neurons. Each neuron's population of presynaptic partners determines how incoming information is processed. A longstanding view of cortical circuits is that a majority of synaptic inputs originate from local networks through horizontal (recurrent) connections. However, the mechanisms by which recurrent networks shape the activity of cortical neurons is largely unknown. Additionally, synaptic and cellular mechanisms proposed by theoretical models rely on studies of the rodent visual cortex, which is increasingly shown to differ from that of carnivores and primates in organization and function. This proposal aims to address these problems by mapping presynaptic excitatory and inhibitory cells of single layer 2/3 neurons and dissect how they act to selectively modulate neural activity in ferret V1 in vivo. This proposal uses a novel combination of advanced optical techniques and electrophysiology, building off the candidates optical create has a deep background in in vivo physiology and imaging in a wide variety of mammalian species and training during the K99 phase. This proposal will the foundation for an innovative, multidisciplinary, and independent research program to establish fundamental principles of cortical circuits, ultimately providing a scaffold for understanding disorders, such as schizophrenia and autism, which show profound impairments in the processing of sensory signals.

雪貂视觉皮层中单层2/3神经元的突触前网络原理 新皮质电路中的单神经元是由兴奋性和抑制性的突触前网络驱动的 神经元。每个神经元的突触前伴侣的人群都决定了如何处理传入信息。 长期以来对皮质电路的看法是，大多数突触输入来自本地网络 水平（经常性）连接。但是，循环网络塑造活性的机制 皮质神经元在很大程度上未知。另外，由理论提出的突触和细胞机制 模型依赖于啮齿动物视觉皮层的研究，该研究越来越多地证明与食肉动物不同 和组织和功能方面的灵长类动物。该建议旨在通过映射解决这些问题 单层2/3神经元的突触前兴奋性和抑制性细胞，并剖析它们如何有选择地作用 在体内调节雪貂V1中的神经活动。该提案使用了高级光学的新颖组合 技术和电生理学，在候选人中建立 光学的 创造 在体内生理学和 在K99阶段进行多种哺乳动物物种的成像和训练。该提议将 创新，多学科和独立研究计划的基础 皮质回路的基本原理，最终为理解疾病提供了脚手架 精神分裂症和自闭症，在感觉信号处理中显示出严重的损害。