PRINCIPLES OF PRESYNAPTIC NETWORKS FOR SINGLE LAYER 2/3 NEURONS IN FERRET VISUAL CORTEX

雪貂视觉皮层单层 2/3 神经元突触前网络原理

• 批准号: 10675228
• 负责人: Benjamin Kyle Scholl
• 金额: $ 12.86万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10675228
• 关键词: Address; Cells; Disease; Electrophysiology (science); Ferrets; Foundations; Goals; Image; Impairment; Neurons; Optics; Phase; Physiology; Population; Primates; Process; Recurrence; Research; Rodent; Role; Schizophrenia; Sensory; Shapes; Signal Transduction; Synapses; Techniques; Theoretical model; Training; Visual Cortex; autism spectrum disorder; excitatory neuron; in vivo; inhibitory neuron; innovation; insight; multidisciplinary; neocortical; novel; presynaptic; programs; relating to nervous system; scaffold; signal processing

Principles of presynaptic networks for single layer 2/3 neurons in ferret visual cortex Single neurons in neocortical circuits are driven by presynaptic networks composed of excitatory and inhibitory neurons. Each neuron's population of presynaptic partners determines how incoming information is processed. A longstanding view of cortical circuits is that a majority of synaptic inputs originate from local networks through horizontal (recurrent) connections. However, the mechanisms by which recurrent networks shape the activity of cortical neurons is largely unknown. Additionally, synaptic and cellular mechanisms proposed by theoretical models rely on studies of the rodent visual cortex, which is increasingly shown to differ from that of carnivores and primates in organization and function. This proposal aims to address these problems by mapping presynaptic excitatory and inhibitory cells of single layer 2/3 neurons and dissect how they act to selectively modulate neural activity in ferret V1 in vivo. This proposal uses a novel combination of advanced optical techniques and electrophysiology, building off the candidates optical create has a deep background in in vivo physiology and imaging in a wide variety of mammalian species and training during the K99 phase. This proposal will the foundation for an innovative, multidisciplinary, and independent research program to establish fundamental principles of cortical circuits, ultimately providing a scaffold for understanding disorders, such as schizophrenia and autism, which show profound impairments in the processing of sensory signals.

雪貂视皮层单层2/3神经元突触前网络的原理 新皮层回路中的单个神经元是由兴奋性和抑制性突触前网络共同驱动的 神经元每个神经元的突触前伙伴群体决定了如何处理传入的信息。 关于皮层回路的一个长期观点是，大多数突触输入来自局部网络， 水平（循环）连接。然而，周期性网络塑造活动的机制， 皮质神经元在很大程度上未知。此外，突触和细胞机制提出的理论 模型依赖于对啮齿类动物视觉皮层的研究，越来越多的研究表明，啮齿类动物的视觉皮层与食肉动物的视觉皮层不同。 和灵长类动物的组织和功能。该提案旨在通过绘制 突触前兴奋性和抑制性细胞的单层2/3神经元，并解剖他们如何发挥作用，选择性 调节雪貂V1体内的神经活动。该提案使用了先进的光学 技术和电生理学， 光学 创建 在体内生理学方面有很深的背景， 在各种哺乳动物物种中进行成像，并在K99阶段进行培训。这项建议会 一个创新的，多学科的，独立的研究计划的基础，建立 皮质回路的基本原理，最终为理解疾病提供了一个支架， 精神分裂症和自闭症，这些疾病在感觉信号的处理方面表现出严重的损伤。