Coordination Core

协调核心

• 批准号: 10700253
• 负责人: David W. Rowe
• 金额: $ 53.5万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-21 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10700253
• 关键词: 3-Dimensional; Address; Adoption; Advocate; Affect; Awareness; Back; Bar Codes; Biology; Biopsy; Cartilage; Cell physiology; Cells; Cellular Structures; Collaborations; Communities; Complex; Confocal Microscopy; Data; Data Analyses; Dental; Discipline; Disease; Educational workshop; Ensure; Environment; Ethnic Origin; Faculty; Gene Expression Profile; Heterogeneity; Histologic; Human; Human BioMolecular Atlas Program; Image; Individual; Institutional Review Boards; Intuition; Joints; Laboratories; Lead; Leadership; Letters; Logistics; Management Information Systems; Maps; Metadata; Minerals; Molecular; Output; Participant; Pathologic Processes; Pathway interactions; Process; Protocols documentation; Publications; Quality Control; RNA; Regulation; Research; Resolution; Resource Development; Resources; Role; Sampling; Scanning; Shapes; Skeleton; Structure; System; Technology; Testing; Tissue Embedding; Tissues; Tooth structure; Visual; arm; base; career development; cell behavior; cell type; data management; data repository; data sharing; expectation; experience; high resolution imaging; histological image; human disease; information display; interest; meetings; member; mineralization; multidisciplinary; multimodality; outreach; preference; programs; recruit; scientific organization; sex; skeletal; skeletal tissue; skills; substantia spongiosa; tool

ABSTRACT: COORDINATION CORE The coordination core has an administrative and scientific function. It will initiate the administrative process of procuring the human skeletal tissues that will be used by the mineralized tissue project. Our LIMS will be modified to capture all the workflow activities from the acquisition to handoff to the mineralized tissue project group. This includes recording the orientation of the sample relative to the tissue of origin, obtaining a µCT scan of the mineral distribution, embedding the tissue for serial sectioning that incorporates four registration points that define a grid structure for cell localization. After widefield histological images are recorded, the same sections are transferred to the mineralization core for the seqFISH studies that identify cell type and individual cell RNA transcriptone profile. Using the same grid system, a 3D cell map will be generated by the data analysis core which in turn will interact with the coordination core to align the histological and 3D cell map. Together the two cores will build a visual representation of the cells within a tissue block that interchangeably relate a specific cell with its transcriptional profile and the active molecular pathways. In an iterative process that will be driven by the coordination core, quality control standards for identification of cell types as well as subdivisions within a cell type will be developed that will serve as the basis for imputing the activity of interacting cells or comparing differences in regulation of cell behavior that underlie dimorphic differences based on sex or ethnicity. As we become proficient in the technologies and data management process of cell mapping, we will shape our data presentation format to that used by other members of the HIVE. By direct interaction with other scientific groups in HuBMAP, we hope to expand the multimodal interrogation of our skeletal tissues and contribute to the technological capabilities of other HuBMAP members. The coordination core will also reach out to the skeletal biology community to make them aware of the resources of the HIVE and provide workshops to help other major skeletal biology groups implement this technology in their research environment. The technologies and concepts of HuBMAP will be transformational for understanding normal and pathological processes in human disease. We want to adapt this experimental platform to tissues of the mineralized skeleton and advocate for its adoption throughout the skeletal research community.

摘要：协调核心 协调中心具有行政和科学职能。它将启动行政 获取将用于矿化组织项目的人体骨骼组织的过程。我们 将修改LIMS，以捕获从采集到移交到 矿化组织项目组。这包括记录样品相对于样品的取向。 原始组织，获得矿物质分布的µCT扫描，包埋组织进行连续 切片包括四个配准点，这些配准点定义了用于细胞定位的网格结构。后 记录宽视野组织学图像，将相同的切片转移到矿化核心 用于鉴定细胞类型和单个细胞RNA转录体谱的seqFISH研究。使用 在同一个网格系统中，数据分析核心将生成3D单元图，而数据分析核心将与 与协调核心对齐组织学和3D细胞图。这两个核心将共同构建一个 组织块内细胞的视觉表示，其可互换地将特定细胞与其 转录谱和活性分子途径。在一个迭代的过程中， 协调核心，用于识别细胞类型以及细分的质量控制标准 在一个细胞类型内将被开发，这将作为基础， 细胞或比较细胞行为调节的差异，这些差异是基于 性别或种族的问题随着我们对细胞技术和数据管理流程的熟练掌握， 映射，我们将塑造我们的数据表示格式，由其他成员的蜂巢使用。通过 与HuBMAP中的其他科学团体直接互动，我们希望扩大多模式 询问我们的骨骼组织，并有助于其他HuBMAP的技术能力 成员协调核心还将接触骨骼生物学社区， 了解蜂巢的资源，并提供研讨会，以帮助其他主要的骨骼生物学团体 在他们的研究环境中实施这项技术。HuBMAP的技术和概念 对于理解人类疾病的正常和病理过程将具有变革性意义。我们 我想让这个实验平台适应矿化骨骼的组织，并倡导其 在整个骨骼研究界被广泛采用。