Coordination Core

协调核心

• 批准号: 10267741
• 负责人: David W. Rowe
• 金额: $ 46.23万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-21 至 2022-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10267741
• 关键词: 3-Dimensional; Address; Adoption; Advocate; Affect; Awareness; Back; Bar Codes; Biology; Biopsy; Cartilage; Cell physiology; Cells; Cellular Structures; Collaborations; Communities; Complex; Confocal Microscopy; Data; Data Analyses; Dental; Discipline; Disease; Educational workshop; Ensure; Environment; Ethnic Origin; Faculty; Gene Expression Profile; Heterogeneity; Histologic; Human; Human BioMolecular Atlas Program; Image; Individual; Institutional Review Boards; Intuition; Joints; Laboratories; Lead; Leadership; Letters; Logistics; Management Information Systems; Maps; Metadata; Minerals; Molecular; Output; Participant; Pathologic Processes; Pathway interactions; Process; Protocols documentation; Publications; Quality Control; RNA; Regulation; Research; Resolution; Resource Development; Resources; Role; Sampling; Scanning; Shapes; Skeleton; Structure; System; Technology; Testing; Tissue Embedding; Tissues; Tooth structure; Visual; arm; base; career development; cell behavior; cell type; data management; data repository; data sharing; expectation; experience; high resolution imaging; histological image; human disease; information display; interest; meetings; member; mineralization; multidisciplinary; multimodality; outreach; preference; programs; recruit; scientific organization; sex; skeletal; skeletal tissue; skills; substantia spongiosa; tool

ABSTRACT: COORDINATION CORE The coordination core has an administrative and scientific function. It will initiate the administrative process of procuring the human skeletal tissues that will be used by the mineralized tissue project. Our LIMS will be modified to capture all the workflow activities from the acquisition to handoff to the mineralized tissue project group. This includes recording the orientation of the sample relative to the tissue of origin, obtaining a µCT scan of the mineral distribution, embedding the tissue for serial sectioning that incorporates four registration points that define a grid structure for cell localization. After widefield histological images are recorded, the same sections are transferred to the mineralization core for the seqFISH studies that identify cell type and individual cell RNA transcriptone profile. Using the same grid system, a 3D cell map will be generated by the data analysis core which in turn will interact with the coordination core to align the histological and 3D cell map. Together the two cores will build a visual representation of the cells within a tissue block that interchangeably relate a specific cell with its transcriptional profile and the active molecular pathways. In an iterative process that will be driven by the coordination core, quality control standards for identification of cell types as well as subdivisions within a cell type will be developed that will serve as the basis for imputing the activity of interacting cells or comparing differences in regulation of cell behavior that underlie dimorphic differences based on sex or ethnicity. As we become proficient in the technologies and data management process of cell mapping, we will shape our data presentation format to that used by other members of the HIVE. By direct interaction with other scientific groups in HuBMAP, we hope to expand the multimodal interrogation of our skeletal tissues and contribute to the technological capabilities of other HuBMAP members. The coordination core will also reach out to the skeletal biology community to make them aware of the resources of the HIVE and provide workshops to help other major skeletal biology groups implement this technology in their research environment. The technologies and concepts of HuBMAP will be transformational for understanding normal and pathological processes in human disease. We want to adapt this experimental platform to tissues of the mineralized skeleton and advocate for its adoption throughout the skeletal research community.

摘要：协调核心 协调核心具有行政和科学功能。它将启动行政管理 获取将被矿化组织项目使用的人类骨骼组织的过程。我们的 将对LIMS进行修改，以捕获从采购到移交到 矿化组织工程组。这包括记录样本相对于 来源组织，获得矿物质分布的微CT扫描，将组织连续包埋 包含四个注册点的切片，这四个注册点定义了用于细胞定位的网格结构。之后 宽视野组织学图像被记录下来，相同的切片被转移到矿化核心 用于确定细胞类型和单个细胞RNA转录音谱的seqFISH研究。使用 同样的网格系统，数据分析核心将生成3D单元图，然后数据分析核心将进行交互 与协调核心对齐组织学和3D细胞图。这两个核心将共同构建一个 组织块中的细胞的视觉表示，它可互换地将特定细胞与其 转录图谱和活性分子途径。在将由以下因素驱动的迭代过程中 用于识别细胞类型和细分的协调核心、质量控制标准 在一种细胞类型内将被开发作为输入相互作用活动的基础 细胞或比较细胞行为调控的差异，这些差异是基于 在性别或种族方面。随着我们对CELL的技术和数据管理流程的熟练掌握 映射后，我们将把我们的数据表示格式调整为该配置单元的其他成员所使用的格式。通过 与HuBMAP中的其他科学团体直接互动，我们希望扩大多模式 询问我们的骨骼组织，并为其他HuBMAP的技术能力做出贡献 会员。协调核心还将联系骨骼生物学社区，以使它们 了解蜂巢的资源，并提供研讨会以帮助其他主要的骨骼生物学组织 在他们的研究环境中实施这项技术。HuBMAP的技术和概念 对于理解人类疾病的正常和病理过程将是变革性的。我们 想要使这个实验平台适应矿化骨骼的组织，并倡导其 在整个骨骼研究社区采用。