Single-Cell Biology Shared Resource

单细胞生物学共享资源

• 批准号: 10675645
• 负责人: Jesse Gillis
• 金额: $ 20.96万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10675645
• 关键词: ATAC-seq; Bioinformatics; Cancer Center; Cancer Center Support Grant; Cancer Center at Cold Spring Harbor Laboratory; Capital; Cell Culture Techniques; Cell Nucleus; Cell Separation; Cells; Cellular biology; Communities; Complex; Copy Number Polymorphism; Custom; Data; Data Analyses; Data Set; Dissociation; Doctor of Philosophy; Educational workshop; Equipment; Evolution; Experimental Designs; Faculty; Gene Expression; Gene Expression Profiling; Generations; Genomics; Genotype; Head; Heterogeneity; Hour; Human Resources; Immune response; Immunologic Receptors; Informatics; Investigation; Laboratories; Learning; Libraries; Lipids; Logistics; Malignant Neoplasms; Manuscripts; Mission; Mutation; Population; Preparation; Process; Proteins; Protocols documentation; Publications; Quality Control; Reagent; Research; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Services; Side; Techniques; Technology; Tissues; Training and Education; Work; cell type; community center; computational pipelines; computerized data processing; design; digital; genomic data; member; novel; programs; proteogenomics; protocol development; single cell analysis; single cell sequencing; tool; transcriptome; transcriptome sequencing; transcriptomic profiling; transcriptomics; tumor heterogeneity; tumor microenvironment; tumor progression

SINGLE-CELL BIOLOGY SHARED RESOURCE – PROJECT SUMMARY/ABSTRACT The Single-Cell Biology Shared Resource was founded in 2017 with the mission of pioneering and deploying cutting-edge single-cell genomics technologies to members of the CSHL Cancer Center and surrounding research community. The Shared Resource is led by Faculty Head Jesse Gilis, Ph.D., and Director Jonathan Preall, Ph.D. The Single-Cell Biology Shared Resource is designed to be a comprehensive, collaborative Shared Resource facility that supports every stage of a single-cell genomics project, including experimental design, protocol development, sample preparation, library construction, sequencing logistics, primary data processing, and in-depth embedded analysis. Shared Resource personnel frequently work side-by-side with researchers, customizing the workflow to meet the unique needs of the project. Examples of the most common single-cell workflows supported by the Shared Resource include: 3’-digital transcriptome profiling, immune receptor profiling, ATAC-sequencing, copy-number variation, proteogenomics, and single-nucleus RNAseq. In addition to standard, established protocols, the Shared Resource assists users in designing and executing custom single- cell workflows with downstream tailored data analysis. The Single-Cell Biology Shared Resource houses and operates capital equipment for handling most stages of common single-cell workflows. A critical component of this Shared Resource’s mission is to provide education and training on the analysis of single-cell genomics data. Semi-annual workshops and regular computational office hours give researchers the opportunity to learn common computational pipelines and become familiar with emerging techniques in data analysis. Since its establishment, the Shared Resource has been used by 12 CSHL Cancer Center laboratories (32% of members), resulting in 5 co-authored publications. In summary, the Single-Cell Biology Shared Resource provides comprehensive bench-to-publication support of both off-the-shelf and customized genomics workflows, dramatically streamlining the process of collecting these challenging and high-impact datasets.

单细胞生物学共享资源-项目总结/摘要 单细胞生物学共享资源成立于2017年，其使命是开拓和部署 尖端的单细胞基因组学技术的CSHL癌症中心和周围的成员 研究社区。共享资源由系主任Jesse Gilis博士领导，导演Jonathan Preall博士单细胞生物学共享资源旨在成为一个全面的，协作的共享资源。 支持单细胞基因组学项目每个阶段的资源设施，包括实验设计， 方案开发，样品制备，文库构建，测序后勤，初级数据处理， 和深入的嵌入式分析。共享资源人员经常与研究人员并肩工作， 自定义工作流程以满足项目的独特需求。最常见的单细胞 共享资源支持的工作流程包括：3 '-数字转录组分析，免疫受体 分析、ATAC测序、拷贝数变异、蛋白质基因组学和单核RNAseq。除了 标准，建立协议，共享资源协助用户设计和执行自定义的单一， 细胞工作流程与下游定制的数据分析。单细胞生物学共享资源库和 操作资本设备，用于处理常见单细胞工作流程的大多数阶段。的关键组成部分 这个共享资源的使命是提供有关单细胞基因组学数据分析的教育和培训。 每半年一次的研讨会和定期的计算办公时间给研究人员提供了学习的机会 常见的计算管道，并熟悉数据分析中的新兴技术。以来 该共享资源已被12个CSHL癌症中心实验室（32%的成员）使用， 共发表了5篇合著文章。总之，单细胞生物学共享资源提供 对现成和定制的基因组学工作流程提供全面的从实验室到出版的支持， 大大简化了收集这些具有挑战性和高影响力的数据集的过程。