Single-Cell Biology Shared Resource

单细胞生物学共享资源

• 批准号: 10270226
• 负责人: Jesse Gillis
• 金额: $ 20.96万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10270226
• 关键词: ATAC-seq; Bioinformatics; Cancer Center; Cancer Center Support Grant; Cancer Center at Cold Spring Harbor Laboratory; Capital; Cell Culture Techniques; Cell Nucleus; Cell Separation; Cells; Cellular Indexing of Transcriptomes and Epitopes by Sequencing; Cellular biology; Communities; Complex; Copy Number Polymorphism; Custom; Data; Data Analyses; Data Set; Dissociation; Doctor of Philosophy; Educational workshop; Equipment; Evolution; Experimental Designs; Faculty; Gene Expression; Gene Expression Profiling; Generations; Genomics; Genotype; Head; Heterogeneity; Hour; Human Resources; Immune response; Immunologic Receptors; Informatics; Investigation; Laboratories; Learning; Libraries; Lipids; Logistics; Malignant Neoplasms; Manuscripts; Mission; Mutation; Population; Preparation; Process; Proteins; Protocols documentation; Publications; Quality Control; Reagent; Research; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Services; Side; Techniques; Technology; Time; Tissues; Training and Education; Work; XCL1 gene; base; cell type; community center; computational pipelines; computerized data processing; design; digital; genomic data; member; novel; programs; proteogenomics; protocol development; single cell analysis; single cell sequencing; tool; transcriptome; transcriptome sequencing; transcriptomics; tumor heterogeneity; tumor microenvironment; tumor progression

SINGLE-CELL BIOLOGY SHARED RESOURCE – PROJECT SUMMARY/ABSTRACT The Single-Cell Biology Shared Resource was founded in 2017 with the mission of pioneering and deploying cutting-edge single-cell genomics technologies to members of the CSHL Cancer Center and surrounding research community. The Shared Resource is led by Faculty Head Jesse Gilis, Ph.D., and Director Jonathan Preall, Ph.D. The Single-Cell Biology Shared Resource is designed to be a comprehensive, collaborative Shared Resource facility that supports every stage of a single-cell genomics project, including experimental design, protocol development, sample preparation, library construction, sequencing logistics, primary data processing, and in-depth embedded analysis. Shared Resource personnel frequently work side-by-side with researchers, customizing the workflow to meet the unique needs of the project. Examples of the most common single-cell workflows supported by the Shared Resource include: 3’-digital transcriptome profiling, immune receptor profiling, ATAC-sequencing, copy-number variation, proteogenomics, and single-nucleus RNAseq. In addition to standard, established protocols, the Shared Resource assists users in designing and executing custom single- cell workflows with downstream tailored data analysis. The Single-Cell Biology Shared Resource houses and operates capital equipment for handling most stages of common single-cell workflows. A critical component of this Shared Resource’s mission is to provide education and training on the analysis of single-cell genomics data. Semi-annual workshops and regular computational office hours give researchers the opportunity to learn common computational pipelines and become familiar with emerging techniques in data analysis. Since its establishment, the Shared Resource has been used by 12 CSHL Cancer Center laboratories (32% of members), resulting in 5 co-authored publications. In summary, the Single-Cell Biology Shared Resource provides comprehensive bench-to-publication support of both off-the-shelf and customized genomics workflows, dramatically streamlining the process of collecting these challenging and high-impact datasets.

单细胞生物学共享资源-项目摘要/摘要