Resource Core C - Skin Genomics, Transcriptomics, and Epigenetics Core
Resource Core C - Skin Genomics, Transcriptomics, and Epigenetics Core
批准号:
10676790
负责人:
Anne Mary Bowcock
金额:
$16.19万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-09-01 至 2026-08-31
关键词:
3-DimensionalATAC-seqAccelerationAddressAffectAlternative SplicingAnatomyAnimal ModelAreaBase SequenceBinding SitesBiologicalBiologyCellsCellular Indexing of Transcriptomes and Epitopes by SequencingCenter Core GrantsChIP-seqChromatinChromosomesCollaborationsCommunitiesConsultationsCoupledDNADNA SequenceDNA sequencingDataData AnalysesDevelopmentDiseaseDisparateDistantElementsEnhancersEnsureEpigenetic ProcessEpitopesExperimental DesignsFacultyFollow-Up StudiesFosteringFundingGene ExpressionGene Expression ProfilingGenesGenetic TranscriptionGenomeGenomicsGoalsHeterogeneityHi-CHistonesHomeostasisHuman ResourcesIndividualInstitutionIntakeLinkLocationMeasurementMedicineMessenger RNAMethodsMolecular ProfilingNucleic Acid Regulatory SequencesPathogenesisPoly(A)+ RNAProcessProtein IsoformsRNARNA SplicingReagentRegulatory ElementResearchResearch PersonnelResearch Project GrantsResolutionResourcesScholarshipService provisionServicesSkinSmall RNASpecimenStandardizationStructureSurveysTechnologyTherapeutic InterventionTissuesTrainingTranscriptValidationVariantVisualizationbiological systemscell typecostdashboarddata managementdesigndisease classificationepigenomeepigenomicsexomeexome sequencinggene networkgenetic analysisgenome sequencinggenome wide association studygenome-widehistone modificationimprovedinsightinstrumentationmembermultiple omicsnovelpromoterrisk variantsingle cell analysissingle cell sequencingsingle cell technologysingle moleculesingle-cell RNA sequencingskin disorderskin organogenesistargeted sequencingtooltranscription factortranscriptometranscriptome sequencingtranscriptomicswhole genome
中文摘要
摘要-资源核心C
英文摘要
Summary – Resource Core C
The results of surveying the Research Community of the SBDRC at Mount Sinai indicated that a top strategic
priority is access to state-of-the-art and integrated methods for genomics, transcriptomics, and epigenetic
analysis of the skin in normal homeostasis and in disease. Core C will provide state-of-the-art start-to-finish
technologies for these cutting-edge approaches. The Core will leverage existing campus resources, such as
instrumentation and extensive expertise at both faculty and researcher levels, to provide technical and
intellectual support to the SBDRC Research Community. Core personnel will be embedded in skin research
labs where they will become familiarized with skin as a biological system and the questions being asked; this
will help to ensure that they can most effectively advise lab personnel on experimental design and services to
address the biological questions; conversely lab personnel will become more familiar with the technological
approaches available to them. Specifically, Core C will provide SBDRC investigators with consultation and
services centered on NexGen technologies. A robust organizational hub (the Smartsheet dashboard) will be
utilized for specimen and project in-take, both internally and externally. As part of Aim 1, Core C will facilitate
access to genomics (exome sequencing, whole genome sequencing and targeted capture) and transcriptomic
technologies (bulk RNA-seq, poly(A) RNA-seq, small RNA-seq) in healthy and diseased skin. Access to long-
read single molecule sequencing (SMRT/PacBio) will facilitate the analysis of structural changes in DNA and
the precise identification of RNA isoforms due to altered splicing. Services in Aim 2 will focus on state-of-the
art epigenomic studies to identify regulatory elements operating in the skin (ATAC-seq, ChIP-seq, CUT&RUN).
Interactions across regulatory elements (enhancers and promoters) will be identified by Hi-C. Integration with
transcriptomic findings will confirm target genes and identify altered networks operating in skin homeostasis
and disease. Aim 3 will focus on cellular heterogeneity, providing single cell technologies for transcript
(scRNA-seq) and epigenetic (scATAC-seq) analyses in single cells. CITE-Seq will provide simultaneous
epitope and transcriptome measurements of single cells. Services from Aim 3 will also include spatial
transcriptomics technology permitting the spatial resolution of RNA-seq data, and thereby the locations of all
mRNAs in individual tissue sections. As novel “omics” technologies are developed that would enhance skin
biology research, they will be incorporated into this Core’s activities. Data will be distributed to Core D where
computational biologists will perform rigorous analysis, visualization and data management. Overall, Core C is
an essential and integral component of the Center with the goal of facilitating scientific discoveries in skin
biology and skin disease areas by providing cutting-edge multi-omics studies to skin researchers.
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Resource Core C - Skin Genomics, Transcriptomics, and Epigenetics Core
-
批准号:10463724
-
项目类别:
-
资助金额:$16.19万
-
财政年份:2021
-
负责人:Anne Mary Bowcock
-
依托单位:
THE GENETICS OF OCULAR MELANOMA
-
批准号:8662732
-
项目类别:
-
资助金额:$38.07万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
The Genetics of Ocular Melanoma
-
批准号:10116295
-
项目类别:
-
资助金额:$63.2万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
The Genetics of Ocular Melanoma
-
批准号:10596996
-
项目类别:
-
资助金额:$64.03万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
The Genetics of Ocular Melanoma
-
批准号:10343767
-
项目类别:
-
资助金额:$63.08万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
THE GENETICS OF OCULAR MELANOMA
-
批准号:8702554
-
项目类别:
-
资助金额:$38.55万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
THE GENETICS OF OCULAR MELANOMA
-
批准号:8826698
-
项目类别:
-
资助金额:$40.38万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
THE GENETICS OF OCULAR MELANOMA
-
批准号:8179029
-
项目类别:
-
资助金额:$59.77万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
The Genetics of Ocular Melanoma
-
批准号:9973279
-
项目类别:
-
资助金额:$62.74万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
THE GENETICS OF OCULAR MELANOMA
-
批准号:8293114
-
项目类别:
-
资助金额:$58.4万
-
财政年份:2011
-
负责人:Anne Mary Bowcock
-
依托单位:
Systems Biology of Psoriasis
-
批准号:7819128
-
项目类别:
-
资助金额:$49.87万
-
财政年份:2009
-
负责人:Anne Mary Bowcock
-
依托单位:
Systems Biology of Psoriasis
-
批准号:7941019
-
项目类别:
-
资助金额:$49.91万
-
财政年份:2009
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-wide SNP association in psoriasis
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批准号:7641224
-
项目类别:
-
资助金额:$7.6万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-Wide SNP Association in Psoriasis
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批准号:8900743
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项目类别:
-
资助金额:$45.17万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-wide SNP association in psoriasis
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批准号:7755377
-
项目类别:
-
资助金额:$62.86万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-Wide SNP Association in Psoriasis
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批准号:8389338
-
项目类别:
-
资助金额:$67.3万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-wide SNP association in psoriasis
-
批准号:7208168
-
项目类别:
-
资助金额:$64.75万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-wide SNP association in psoriasis
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批准号:7369805
-
项目类别:
-
资助金额:$63.37万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-Wide SNP Association in Psoriasis
-
批准号:9762432
-
项目类别:
-
资助金额:$3.85万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
Genome-Wide SNP Association in Psoriasis
-
批准号:8829659
-
项目类别:
-
资助金额:$45.25万
-
财政年份:2007
-
负责人:Anne Mary Bowcock
-
依托单位:
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