MicroRNA Networks in self-sustaining type 2 airway niches in asthma
哮喘自我维持 2 型气道生态位中的 MicroRNA 网络
基本信息
- 批准号:10681275
- 负责人:
- 金额:$ 54.26万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-08-15 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:AccelerationAdoptedAffectAirAllergensAllergic inflammationAnatomyAsthmaBasic ScienceBinding SitesBiochemicalBioinformaticsBiologyCCR6 geneCell Differentiation processCell LineCell ReprogrammingCellsCharacteristicsCollaborationsCritical PathwaysCytometryDataDevelopmentDiseaseDrug or chemical Tissue DistributionEpithelial CellsEpitheliumExperimental ModelsFamilyFrequenciesFunctional disorderGene ExpressionGene Expression ProfilingGenesHeterogeneityHomeostasisHumanHyperactivityImmuneImmunologicsIn VitroInflammationKnockout MiceLaboratoriesLiquid substanceLungMapsMediatingMicroRNAsMolecularMolecular TargetMucous body substanceMusNaturePathogenesisPathologicPathologic ProcessesPathologyPathway interactionsPeripheralPopulationProductionPropertyPulmonary InflammationPulmonary PathologyRNARegulatory T-LymphocyteResearchRoleSiteSystemT cell differentiationT-LymphocyteT-Lymphocyte SubsetsTechnologyTestingThymus GlandTissuesTranslational Researchairway epitheliumairway inflammationairway obstructionallergic airway inflammationcell typecytokinegene networkgene regulatory networkgenome editinghigh dimensionalityimmunopathologyimmunoregulationinnovationmiRNA expression profilingmolecular markermouse modelmucin hypersecretionmucus hypersecretionnovelpreventprogramsresponsesingle cell mRNA sequencingtranscriptomics
项目摘要
Project Summary
Th2 high asthma is defined by persistent airway inflammation that is driven by cytokine crosstalk between
epithelial cells and tissue resident innate and adaptive immune cells. The central theme of our collaborative
research program is that the focal nature of type 2 inflammation that occurs in asthma reflects the development
of persistent type 2 airway niches containing reprogrammed epithelial and immune cells. Disrupting these niches
may durably impact asthma pathogenesis. Under normal conditions, transient type 2 responses operate to
maintain epithelial barrier function. In asthma, regulatory mechanisms that dampen these responses fail, and
type 2 inflammation with epithelial cell reprogramming and mucin hypersecretion persists at focal sites in the
airways. The central objective of this project is to define molecular determinants of cell reprogramming that
sustain persistent immunopathology in Th2-high asthma.
The proposed studies focus on two cell types whose programming directly affects this pathological process:
T regulatory (Treg) cells and airway epithelial cells. Our approach builds upon preliminary data indicating that
type 2 inflammation and lung dysfunction correlate inversely with the frequency of a subset of airway Tregs, and
the positive identification of distinct miRNA families that control the programming of Tregs and epithelial cells.
The project is organized into three aims: In Aim 1, we will use single cell sequencing, mRNA and miRNA profiling
and mass cytometry to define airway Treg populations, and to compare Treg subsets in persistent airway type 2
niches marked by focal sites of mucus impaction with those that populate unaffected airways. In Aim 2, we will
dissect Treg programming using miRNA-directed pathway discovery, a novel experimental framework for probing
miRNA:target gene networks. A similar approach will be applied in human airway epithelial cells in Aim 3 to
reveal miRNA:target networks that control the cell reprogramming and mucin hypersecretion that marks airway
type 2 niches in asthma. If successful, the proposed research will uncover novel genes and pathways critical to
the pathology of asthma, advance our understanding how immune regulation mechanisms fail in type 2 airway
niches, and suggest strategies to disrupt the inflammation that sustain this disease.
项目概要
Th2 高哮喘的定义是由细胞因子串扰驱动的持续性气道炎症。
上皮细胞和组织驻留先天性和适应性免疫细胞。我们合作的中心主题
研究计划是,哮喘中发生的 2 型炎症的焦点性质反映了哮喘的发展
含有重新编程的上皮细胞和免疫细胞的持续性2型气道微环境。破坏这些利基市场
可能会持久影响哮喘发病机制。在正常情况下,瞬态 2 型响应可实现
维持上皮屏障功能。在哮喘中,抑制这些反应的调节机制失效了,并且
伴有上皮细胞重编程和粘蛋白分泌过多的 2 型炎症持续存在于
航空公司。该项目的中心目标是定义细胞重编程的分子决定因素
维持 Th2 高哮喘的持续免疫病理学。
拟议的研究重点关注两种细胞类型,它们的编程直接影响这种病理过程:
调节性 T (Treg) 细胞和气道上皮细胞。我们的方法建立在初步数据的基础上,表明
2 型炎症和肺功能障碍与气道 Tregs 子集的频率呈负相关,并且
积极鉴定出控制 Tregs 和上皮细胞编程的不同 miRNA 家族。
该项目分为三个目标: 在目标 1 中,我们将使用单细胞测序、mRNA 和 miRNA 分析
和质谱流式细胞仪来定义气道 Treg 群体,并比较 2 型持续性气道中的 Treg 亚群
以粘液嵌塞的焦点部位为标志的壁龛,以及未受影响的气道中的壁龛。在目标 2 中,我们将
使用 miRNA 引导的通路发现来剖析 Treg 编程,这是一种新颖的探测实验框架
miRNA:目标基因网络。类似的方法将应用于目标 3 中的人气道上皮细胞
揭示 miRNA:控制细胞重编程和标记气道的粘蛋白过度分泌的靶网络
2型哮喘的利基。如果成功,拟议的研究将发现对生命至关重要的新基因和途径
哮喘的病理学,加深我们对免疫调节机制如何在 2 型气道中失效的理解
利基,并提出了破坏维持这种疾病的炎症的策略。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Karl Mark Ansel其他文献
Karl Mark Ansel的其他文献
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{{ truncateString('Karl Mark Ansel', 18)}}的其他基金
Restorative practice in repairing harm and promoting safe and inclusive practices in the laboratory.
修复伤害和促进实验室安全和包容性实践的恢复性实践。
- 批准号:
10393434 - 财政年份:2020
- 资助金额:
$ 54.26万 - 项目类别:
MicroRNA Networks in self-sustaining type 2 airway niches in asthma
哮喘自我维持 2 型气道生态位中的 MicroRNA 网络
- 批准号:
10226877 - 财政年份:2012
- 资助金额:
$ 54.26万 - 项目类别:
MicroRNA Networks in self-sustaining type 2 airway niches in asthma
哮喘自我维持 2 型气道生态位中的 MicroRNA 网络
- 批准号:
10006352 - 财政年份:2012
- 资助金额:
$ 54.26万 - 项目类别:
MicroRNA Networks in self-sustaining type 2 airway niches in asthma
哮喘自我维持 2 型气道生态位中的 MicroRNA 网络
- 批准号:
10472538 - 财政年份:2012
- 资助金额:
$ 54.26万 - 项目类别:
Role of miRNAs in Th2-Driven inflammation in Asthma
miRNA 在 Th2 驱动的哮喘炎症中的作用
- 批准号:
8309235 - 财政年份:2011
- 资助金额:
$ 54.26万 - 项目类别:
MicroRNA directed pathway discovery in allergy and asthma
MicroRNA 指导过敏和哮喘通路的发现
- 批准号:
10840232 - 财政年份:2011
- 资助金额:
$ 54.26万 - 项目类别:
Cas9 RNP targeting cis-regulatory elements in lymphocytes
Cas9 RNP 靶向淋巴细胞中的顺式调控元件
- 批准号:
10630567 - 财政年份:2011
- 资助金额:
$ 54.26万 - 项目类别:
MicroRNA directed pathway discovery in allergy and asthma
MicroRNA 指导过敏和哮喘通路的发现
- 批准号:
10433928 - 财政年份:2011
- 资助金额:
$ 54.26万 - 项目类别:
Role of miRNAs in Th2-Driven inflammation in Asthma
miRNA 在 Th2 驱动的哮喘炎症中的作用
- 批准号:
8870413 - 财政年份:2011
- 资助金额:
$ 54.26万 - 项目类别:
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